TRAIL与kallistatin联合表达载体的构建及抗肿瘤活性分析

为研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)与组织激肽释放酶结合蛋白(kallistatin)联合用药的抗肿瘤作用,构建TRAIL与kallistatin双表达的重组质粒pAM-CAG-Kal-IRES-TRAIL,将重组质粒转染A549,LO-2,NCI-H446和Hela细胞,考察其抗肿瘤活性.实验结果表明:构建的双表达载体能同时表达TRAIL与kallistatin,且均能分泌至培养基中;TRAIL与kallistatin联合表达对肿瘤细胞活力的抑制作用明显增强,诱导肿瘤凋亡的作用也明显增强,说明联合表达TRAIL与kallistatin能够增强抗肿瘤活性.     

韩氏溶出-改善利托那韦片的流体力学和溶出行为

溶出是制药行业中常用的研发手段和质量控制方法,常用的溶出方法包括篮法和桨法。由于药物在溶出装置中落点位置的不同和堆积等原因,溶出测试仍然受到重复性和准确性问题的困扰。提出溶出设备新的改良方式,并命名为"韩氏溶出"。通过改良的溶出方法对难溶性药物利托那韦原研制剂溶出考察和重现性分析。结果表明,改进的溶出装置减小了溶出装置的物理参数对溶出试验的变异性的影响,可大幅度提升重现性,避免了因测试本身的性质导致溶出试验失败的情况,从而满足质量控制的要求,以保证了研究者获得更好的体内外相关性。对目前药物制剂开发及药物一致性评价中的溶出曲线对比有较大的帮助。     

Short-range clutter suppression method combining oblique projection and interpolation in airborne CFA radar

The airborne conformal array(CFA)radar's clutter ridges are range-modulated,which result in a bias in the estimation of the clutter covariance matrix(CCM)of the cell under test(CUT),further,reducing the clutter suppression performance of the airborne CFA radar.The clutter ridges can be effectively compensated by the space-time separation interpolation(STSINT)method,which costs less computation than the space-time interpolation(STINT)method,but the performance of interpolation algorithms is seriously affected by the short-range clutter,especially near the platform height.Location distributions of CFA are free,which yields serious impact that range spaces of steering vector matrices are non-orthogonal complement and even no longer disjoint.Further,a new method is proposed that the shortrange clutter is pre-processed by oblique projection with the intersected range spaces(OPIRS),and then clutter data after being pre-processed are compensated to the desired range bin through the STSINT method.The OPIRS also has good compatibility and can be used in combination with many existing methods.At the same time,oblique projectors of OPIRS can be obtained in advance,so the proposed method has almost the same computational load as the traditional compensation method.In addition,the proposed method can perform well when the channel error exists.Computer simulation results verify the effectiveness of the proposed method.     

健康城市视角下应对突发公共事件的城市设计策略

 结合中国城市应对新冠肺炎疫情的成功经验及缺憾，以创造健康、宜居、韧性、生态的城市环境为目标，设计“区域-系统-节点”三类健康城市设计路径；提出“生态健康-生活健康-治愈健康-环境健康-设施健康-救治健康”六位一体应对突发公共事件的城市设计策略，并细化为健康城市在城市空间上的形象化设计表达。     

吡喃葡萄糖基咪唑啉盐表面活性剂的波谱学数据分析

以1-(2,6-二甲基苯基)-1H-咪唑与2-溴乙基葡萄糖苷为原料,一步合成了新化合物溴化1-(2,6-二甲基苯基)-3-[2-(2,3,4,6-四-O-乙酰基-β-D-吡喃葡萄糖基氧基)-乙基]-咪唑盐(化合物1),是一种咪唑啉盐表面活性剂。化合物1的结构中存在糖基,含有多个手性碳原子,导致其核磁共振氢谱和碳谱较为复杂。我们运用液相色谱-高分辨质谱(LC-HRMS)和元素分析(EA)确定了其化学组成,随后通过液体核磁共振波谱技术(包括1D和2D核磁共振手段,如~1H NMR、~(13)C NMR、DEPT90、DEPT135、~1H-~1H COSY、~1H-~(13)C HSQC、~1H-~(13)C HMBC)对化合物1的氢谱和碳谱信号进行了表征,通过解析对其结构进行了详尽归属。     

Monopulse instantaneous 3D imaging for wideband radar system

To avoid the complicated motion compensation in interferometric inverse synthetic aperture(InISAR)and achieve realtime three-dimensional(3 D)imaging,a novel approach for 3 D imaging of the target only using a single echo is presented.This method is based on an isolated scatterer model assumption,thus the scatterers in the beam can be extracted individually.The radial range of each scatterer is estimated by the maximal likelihood estimation.Then,the horizontal and vertical wave path difference is derived by using the phase comparison technology for each scatterer,respectively.Finally,by utilizing the relationship among the 3 D coordinates,the radial range,the horizontal and vertical wave path difference,the 3 D image of the target can be reconstructed.The reconstructed image is free from the limitation in InISAR that the image plane depends on the target's own motions and on its relative position with respect to the radar.Furthermore,a phase ambiguity resolution method is adopted to ensure the success of the 3 D imaging when phase ambiguity occurs.It can be noted that the proposed phase ambiguity resolution method only uses one antenna pair and does not require a priori knowledge,whereas the existing phase ambiguity methods may require two or more antenna pairs or a priori knowledge for phase unwarping.To evaluate the performance of the proposed method,the theoretical analyses on estimation accuracy are presented and the simulations in various scenarios are also carried out.     

论油气本源共同体——老油区发现新储量的探索思路

以克拉玛依油区为实例,提出了油气本源共同体新概念。指出深埋于新疆克拉玛依加依尔山下八大地质现象(巨大推覆体的加依尔山及其犁式断裂系统、玛湖新凹陷、山下古凹陷、中下地壳巨厚的低速高导层及其两侧的壳幔断裂、新疆隆起最高的莫霍面、深部上地幔软流圈形成隆起幅度达65 km的地幔柱),实际上就是生油—运移—散失—聚集—成藏—成带的隐工程。剖析这些深部构造后发现,裸露于加依尔山边百公里长多种类型丰富而罕见的油气苗和隐伏于地下沿克乌断裂带、玛湖断裂带聚集的油气富集带之油源,并非仅仅来自玛湖新凹陷,而更主要是来自深部三大烃灶(古凹陷内沉积物转化成烃、壳内低速高导层内由一氧化碳和氢合成幔源油气、地幔柱内由深部地幔流体聚合成烃碱流体),靠深、浅二套断裂系统上下沟通,形成既各自独立又相互联系、相互作用的不可分离的统一整体,即油气本源共同体。在我国华北、渤海湾、四川等油区都发育油气本源共同体,虽形态各异,但大同小异,提出应改变过去从上往下找油,顾上不顾下的传统做法,改为从下往上找油,上下兼顾的新思路。     

M_xZn_(3-0.5x)(PO_4)_2(M=Na,K)长余辉发光材料的发光性能和机理探讨

采用高温固相法制备以缺陷为发光中心的淡蓝绿色长余辉发光材料M_xZn_(3-0.5x)(PO_4)_2(M=Na,K)。XRD分析结果表明,M_xZn_(3-0.5x)(PO_4)_2的主要衍射峰与α-Zn_3(PO_4)_2的值相吻合。Na_(0.08)Zn_(2.96)(PO_4)_2激发峰位于332 nm处,发射峰在420～550 nm,最大值位于460 nm处,目测余辉时间达4 h。通过热释光曲线表征分析陷阱数量并计算了陷阱深度,分析表明,Na~+掺杂可以增强Zn_3(PO_4)_2在低温处的氧空位缺陷浓度,改善材料的陷阱深度,从而使材料发光。     

玉米芯-污泥基改性复合活性炭对废水中Cd~(2+)的吸附特性

本文以废弃物普通玉米芯和城市污泥为研究原料,通过碳酸钾改性、碳化方法,制备玉米芯-污泥基改性复合活性炭吸附剂。同时,探究了不同复配比例吸附剂受添加剂量、粒径、废水初始浓度等因素的影响程度,以优化复合吸附剂的复配比,并利用扫描电子显微镜(SEM/EDS)、傅里叶红外光谱等手段进行检测分析。研究表明,玉米芯与污泥的复配比为1∶3、粒径为0.83mm吸附剂受废水初始浓度影响较大,当废水初始浓度为50mg/L污泥基改性复合活性炭可适用于吸附低浓度重金属Cd~(2+)废水,实现低浓度Cd~(2+)的固化转移。     

胞嘧啶脱氨基酶APOBEC1研究进展

为全面理解载脂蛋白B mRNA(ApoB mRNA)编辑酶催化多肽-1(APOBEC1)的作用机制,介绍了APOBEC1和ApoB mRNA的蛋白及核酸序列,总结并绘制了APOBEC1与不同的辅助蛋白的结合模型,阐述了APOBEC1催化ApoB mRNA第6 666位的胞嘧啶(C_(6666))脱氨基化分子机制.列举了啮齿动物APOBEC1抑制多种逆转录病毒的研究报道,介绍了兔源APOBEC1结合人类免疫缺陷病毒1(HIV-1)的病毒粒子并编辑病毒基因组的机理.同时介绍了APOBEC1通过编辑胞嘧啶或与AU富集元件(ARE)结合来调控癌症等疾病相关的细胞因子表达.