组织工程人角膜内皮的研究进展

从种子细胞的来源和载体支架的制备两个方面对组织工程人角膜内皮的研究进展及其前景进行综述。     

悬挑式脚手架方案优化

介绍了对房建工程悬挑式脚手架搭设方案进行优化的具体细节,使其更适合于目前房建项目施工。     

Fabrication and Properties of Porous Film/Fabric Composites for Vascular Scaffold Application

A composite vascular scaffold combining textile reinforced structure and biodegradable polymer is introduced, which may possess high porosity and connectivity. Moreover, the porous size could be controlled. The proposed scaffold consists of a warp-knitted poly （ethylene terephthalate ） （PET） fabric with well-defined macropores, which is embedded with a porous biodegradable polymer membrane. The aim of this paper is to study the fabrication and properties of porous polymer membrane through optimizing the parameter of composite methods from freeze drying/particle leaching （ FD/PL ） and gas foaming/particle leaching （ GF/PL ）, subsequently combining with the warp-knitted fabric. Weighing method was utilized to analyze the porosity of the samples and the scanning electron microscope （SEM） images were taken to observe the porous structure of the vascular membrane. In addition, ,the static contact angle （CA） was measured to estimate the hydrophilicity of the samples, and the tensile testing of the composites was performed on the universal mechanical tester. Furthermore, the water permeability of the membrane was also calculated. The results showed that the porosity and pore connectivity of the vascular membrane were diverse, became of solution concentration, particle size, ratio of content, etc. Meanwhile, the stress-strain curves and the bursting strength showed the different mechanical properties among composite scaffolds in different structures.     

阅读教学改革新途径--阅读策略教学

文章着重介绍了阅读技能观到阅读建构观的转化 ,阅读教学从技能训练到策略教学的发展。并围绕两个问题阐述如何开展阅读策略教学。这两个问题是 :(1)教师在策略教学中应教给学生什么 ?(2 )策略教学应通过什么途径实现 ?以期对当前的语文课程改革提供借鉴。     

造孔粒子形貌对磷酸钙骨水泥支架材料结构与性能的影响

采用可溶粒子造孔法,结合冷等静压成型技术,分别以立方体的氯化钠晶体和短棒状的谷氨酸钠晶体为造孔粒子制备出具有较高孔隙率和良好抗压强度的磷酸钙骨水泥组织工程支架材料,通过X射线衍射分析、扫描电镜分析及力学性能测试,研究了所制备的支架材料的物相组成及颗粒形貌对支架材料显微结构和力学性能的影响.结果表明:两种造孔粒子制得的支架材料的主晶相均是弱结晶的羟基磷灰石;造孔粒子的形貌决定了所制备的支架的孔隙形貌,并影响支架的孔隙率和力学性能;在造孔粒子加入量相同的情况下,谷氨酸钠晶体作为造孔粒子制备的支架的孔隙率更高,但强度更低;由于在制备过程中引入了等静压处理,支架材料具有良好的强度.     

地铁车站主体结构满堂脚手架施工技术探讨

针对地铁主体结构施工,提出了采用满堂脚手架支模一次浇筑侧墙、板梁混凝土的施工办法,并对施工过程中的参数设置、施工顺序、注意事项等做了较为详细的介绍。本方法与传统大钢模施工侧墙技术相比具有造价低、速度快、可以减少施工缝等特点,对同类型工程施工具有一定的借鉴作用。     

套管承插式脚手架节点力学性能研究

为了揭示套管承插式节点的半刚性特性、计算方法及其对节点及脚手架承载力的影响,本文结合相关试验数据、数值仿真和理论分析,研究了节点连接棒的伸入长度、连接方式对节点刚度产生的影响以及节点刚度对脚手架承载力的影响。分析结果表明：节点连接棒的长度为在12cm范围内时,伸入长度的变化决定了节点的刚度大小,超过12cm时,节点刚度的数值基本不受连接棒伸入长度影响;固定式连接棒的节点转动刚度相对于活动式的提高11%左右,进一步模拟得到采用三点焊接方式的节点刚度最优。最后选取5组节点刚度数据对脚手架进行仿真计算,得出节点刚度对结构极限承载力大小的影响能达到40%,说明节点刚度对脚手架承载力有显著影响。     

一种新型骨组织工程材料——CPP纤维的制备和性能

采用化学成分与人体骨矿物质相近的磷酸钙为主要原料,通过添加适量的稳定剂、阻降剂研制出具有可控降解速率的聚磷酸钙纤维。测试了聚磷酸钙纤维的力学性能、降解性能、毒理学性能和生物相容性能,讨论了聚磷酸钙纤维的降解机理。     

关于(A6,A11)-双位点突变胰岛素原作为外源肽呈递骨架可行性的研究

通过重组DNA技术,以(A6,A11)-双突变胰岛素原分子为支架构建了含有外源抗栓肽功能区的嵌合分子,并在大肠杆菌进行了表达和纯化.对嵌合分子进行胰岛素受体结合活性、放射免疫活性及抗血小板聚集活性分析,结果表明该嵌合分子具有较强的血小板聚集抑制活性,而无胰岛素生物活性,证明以(A6,A11)-双突变胰岛素原作为外源肽序列呈递骨架是可行的.     

Development of a hybrid scaffold and a bioreactor for cartilage regeneration

We developed a hybrid scaffold and a bioreactor for cartilage regeneration. The hybrid scaffold was developed as combination of two components： a biodegradable framework and hydrogel-containing chondrocytes. We performed the MTT cell proliferation assay to compare the proliferation and viability of chondrocytes on three types of scaffolds： an aiginate gel, the hybrid scaffold, and an alginate sponge. Cells were encapsulated in 2% agarose gel. The bioreactor consisted of a circulation system and a compression system. We performed dynamic cell culture on these agarose gels in the bioreactor for 3 days.