芫花根的酚类成分及其免疫调节活性

采用硅胶和Sephadex LH-20柱层析技术和多种波谱分析方法．从芫花根中分离鉴定出7种酚类化合物．它们分别为伞形花内酯(1)、瑞香苷(2)、西瑞香素(3)、芫花素(4)、芫根苷(5)、毛瑞香素B(6)和毛瑞香素G(7)，其中-4和7为首次从芫花根中分离得到．上述化合物的免疫调节活性通过其对小鼠巨噬细胞分泌白介素-1β(IL-1β)，小鼠T淋巴细胞增殖及分泌白介素-2(IL-2)等进行检测．结果表明．20～30mg／L的化合物3～7对巨噬细胞分泌IL-1β，T淋巴细胞增殖及分泌IL-2有显的提升作用．     

Inducible nitric oxide synthase expression is related to angiogenesis,bcl—2 and cell proliferation in hepatocellular carcinoma

In this study,we examined the expression of inducible nitric oxide synthase(iNOS) and vascular endothelial growth factor(VEGF) by immunohistochemical staining in 76 tissue sections collected from bepa-tocellular carcinoma(HCC) patients undergoing hepatectomy.Microvascular density (MVD) was determined by counting endothelial cells immunostained using anti-CD34 antibody.We performed DNA-flow cytometric analyses to elucidate the impact of iNOS and VEGF expression on the cell cycle of HCC.Most of the HCC cells that invaded stroma were markedly immunostained by iNOS antibody.The iNOS stain intensity of the liv-er tissue close to the tumor edge was stronger than that of HCC tissue,and the strongest was the hepatocytes colser to the tumor tissue.However,iNOS expression in 10 normal hepatic samples was undetectable.VEGF positive expression ratio was 84.8% in iNOS positive expression cases,and the ratio was 35.3% in negative cases.There was significant correlation(P=0.000) between iNOS and VEGF expression.Moreover,iNOS expression was significantly associated with bcl-2 and MVD,but without p53 expression.DNA-flow cytometric analyses showed that combined expression of iNOS and VEGF had significant impact on the cell cycle in HCC.PI(Proliferating Index) and SPF(S-phase fraction)in the combined positive expression of iNOS and VEGF group was significantly higher than that in the combined negative group.The present findings suggested that iNOS expression was significantly associated with angiogenesis,bcl-2 and cell proliferation of HCC.     

不同物理环境对体外培养动物细胞的影响

本文介绍了生物物理领域中不同物理环境对体外培养的动物细胞增殖方面的研究进展。着重介绍了各种电磁场对不同细胞增殖的影响；失重、超重刺激对细胞增殖的影响；激光照射对细胞增殖的影响；以及不同力学环境对细胞分裂、增殖的影响。     

兔骨髓基质细胞的分离培养研究

目的分离培养兔骨髓基质细胞,观察其体外生长特性.方法用密度梯度离心结合贴壁培养法分离兔骨髓基质细胞,进行体外培养,传代扩增,测定其生长曲线,用相差显微镜、细胞化学染色观察细胞生物学性状及功能特点.结果密度梯度离心结合贴壁培养法可有效分离并且纯化兔的骨髓基质细胞.传代细胞均一性较好,呈纺锤形,细胞增殖快,加入地塞米松(Dxm 10-5 mmol/L),β-甘油磷酸钠(β-GP 2 mmol/L)、L-抗坏血酸(AA 50 mg/L)可诱导BMSCs分化为成骨细胞,von kossa法检测骨钙素阳性.结论体外培养的骨髓基质细胞生长稳定,增殖力强,可诱导分化.     

人参皂甙Rb1对体外培养的小鼠支持细胞增殖作用的研究

观察人参皂甙Rb1(ginsenoside Rb1,GRb1)对体外培养的小鼠支持细胞增殖能力的影响,探讨其促进精子发生的作用机制.采用多次贴壁法纯化原代培养的支持细胞,取体外培养第2代第3天的支持细胞,利用MTT比色分析法和Brdu增殖细胞标记法,检测不同质量浓度GRb1对体外培养支持细胞增殖的影响.20 mg/LGRb1对支持细胞增殖有明显促进作用.GRb1能维持小鼠支持细胞的存活,并在适当质量浓度范围内可以明显促进支持细胞的增殖,从而为进一步探讨GRb1的药理作用及精子发生的机理提供一些研究基础.     

Effect of Quercetin on Breeding and Apoptosis of Cervical Cancer HeLa Cell and on Growth of Transplanted Tumor in Nude Mice

Effect of quercetin on HeLa cell system of cervical cancer was studied by methods of MTT and Annexin V-FITC/PI. The results show that quercetin has functions of inhibiting breeding of HeLa cells and inducing apoptosis of the cells. The total apoptosis rate is positively proportional to reaction duration and concentration of quercetin used. The maximum apoptosis rate being （88.76±2.35）% was obtained when the concentration was 50.0 μmol/L and the cells were treated with quercetin for 72 hours. Based on establishing a model of tumor of cervical cancer transplanted into nude mice, quercetin of different concentrations was injected into abdominal cavity of nude mice and situation of tumor growth was reviewed. The result showed that with quercetin concent＇ration increasing from 0 to 100.0 μmol/L, the transplantation volume and weight of the tumors decreased from （279.59±70.58） mm^3 and （0.145±0.019） g to （128.72±36.12） mm^3 and （0.089± 0.019） g respectively, while apoptosis rate of the transplanted tumor increased from （9.63±1.85）% to （34,98±0.47）%, which proved that quercetin inhibited increment of volume and weight of transplanted tumor in nude mice bodies.     

小鼠胚胎发育中期肝细胞增殖与凋亡

探讨小鼠胚胎中期肝发育过程细胞增殖与凋亡的变化规律、相关基因P21及Ki-ras在肝发育中的表达意义。在胚胎中期的小鼠肝组织中，采用免疫组织化学及切口末端标记法，可检测到小鼠肝发育过程中的细胞增殖与凋亡相伴存在，这与肝发育变化密切相关。用原位杂交的方法检测到P21时,Ki-ras基因在胚胎肝发育中期并没有表达，说明这两个细胞周期调控基因可能在胚胎肝发育中期并不起作用，而与其他的基因有关。     

细胞及其增殖、调控机制的发现历程

细胞是生命结构与功能的基本单位。生命科学发展到今天 ,人类已有可能鉴定出完成细胞功能所需的全部基因。但有关的基因是如何作用以产生细胞组织的 ?细胞内的所有基因是按什么规则协同作用的 ?进一步弄清楚这些问题 ,将有助于我们充分了解细胞 ,从而充分了解生命本身     

雌激素受体α亚细胞定位及其与细胞生长的相关性

雌激素受体（Estrogen Receptor，ER）是核受体（Nucleus Receptor，NR）超家族成员，能够被其配体雌二醇（Steroid hormone17-β-estradiol，E2）激活．ER有两种亚型，ERα和ERβ，由4个不同的功能域组成．通过构建ERα的不同缺失突变体（ER△LBD、ER△DBD和ER△Hinge）来研究不同功能域对细胞增殖及在E2刺激下对ERα亚细胞定位的影响．结果表明，ERα呈核多浆少的分布，ER△LBD为核定位．ER△DBD为核多浆少分布，ER△Hinge则主要分布在胞浆．进一步分析表明，E2诱导的ERα入核是核定位序列（Nuclear Location Signal，NLS）依赖性．此外，我们还发现，ERα能够增强293T细胞增殖，且这种促增殖效应可能依赖于ERα在细胞核与胞浆的正常转运．     

补阳还五汤乙醇、氯仿提取物挥发性成分及调控鼠骨髓间充质干细胞活性研究

采用GC-MS对中药复方补阳还五汤的乙醇及氯仿提取物进行分析,结果发现2种溶剂提取物中挥发性成分大致相同;通过对补阳还五汤2种溶剂提取物进行骨髓间充质干细胞增殖活性的测定,发现2种溶剂提取物均促进鼠骨髓间充质干细胞的增殖(P＜0.05).