Label-free and real-time detection of specific binding of IgG proteins by oblique-incidence reflectivity difference method

The entire interaction process between anti-mouse immunoglobulin-G (IgG) and mouse IgG with a concentration range from 1 to 0.25 mg/mL has been label-free detected by the oblique incidence reflectivity difference (OIRD) method.We achieved the two-dimensional scanning images of OIRD Re{△ p △s } intensities before and after interactions as well as the curves of reaction dynamic processes.The experimental results suggest that OIRD method not only can label-free distinguish the different concentrations of proteins and detect biological microarrays,but also can real-time monitor the kinetic processes of biomolecular interactions.     

祖国统一大业与中华民族凝聚力

：中国传统文化历来注重国家统一，统一是中国历史发展的主流。实现祖国统一必须增强中华民族的凝聚力。中华民族具有极强的凝聚力，两岸统一是民心所向、大势所趋。台湾回归祖国，不仅对两岸经济社会发展有诸多好处，而且反过来会在很大程度上增强中华民族凝聚力。为了实现祖国统一，应特别注意做好四方面的工作：一、推进中国特色社会主义建设，提升综合国力；二、深化政治体制改革，加强民主化、法制化建设；三、坚持和完善“一国两制”方针，确保香港、澳门的稳定与繁荣；四、加强两岸经济文化交流，与“台独”势力进行坚决斗争。     

Genomic and genic sequence variation in synthetic hexaploid wheat （AABBDD） as compared to their parental species

In order to understand the genomic changes during the evolution of hexaploid wheat, two sets of synthetic hexaploid wheat from hybridization between maternal tetraploid wheat （AABB） and paternal diploid goat grass （DD） were used for DNA-AFLP and single strand conformation polymorphism （SSCP） analysis to determine the genomic and genic variation in the synthetic hexaploid wheat. Results indicated that more DNA sequences from paternal diploid species were eliminated in the synthetic hexaploid wheat than from maternal tetraploid wheat, suggesting that genome from parental species of lower ploidity tends to be eliminated preferentially. However, sequence variation detected by SSCP procedure was much lower than those detected by DNA-AFLP, which indicated that much less variation in the genic regions occurred in the synthetic hexaploid wheat, and sequence variations detected by DNA-AFLP could be derived mostly from non-coding regions and repetitive sequences. Our results also indicated that sequence variation in 4 genes can be detected in hybrid F1, which suggested that this type of sequence variation could be resulted from distant hybridization. It was interesting to note that 3 out of the 4 genes were mapped and clustered on the long arm of chromosome 2D, which indicated that variation in genic sequences in synthetic hexaploid wheat might not be a randomized process.     

一类环形区域上的椭圆问题

文章讨论一类环形区域上的Laplace边值问题-△u=f（u），x∈B1＼Bt；u=0，x∈B1；u/n=0,x∈Bt利用Leggett—Williams三解定理获得至少三个非负径向解的存在性．     

NECESSARY AND SUFFICIENT CONDITION FOR GLOBAL CONTROLLABILITY OF A CLASS OF AFFINE NONLINEAR SYSTEMS

In this paper, we investigate the global controllability of a class of n-dimensional affine nonlinear systems with n- 1 controls and constant control matrix. A necessary and sufficient condition for its global controllability has been obtained by using the methods recently developed. Furthermore, we generalize the above result to a class of affine nonlinear systems with a block-triangular-like structure. Finally, we will give three examples to show the applications of our results.     

用水萃取法提取仙人果中的玫瑰红色素

以水为萃取剂,加入L-抗坏血酸作抗氧化剂、果胶酶降解果胶、改性甲壳胺吸附蛋白质.提取仙人果中的玫瑰红色素,此法萃取率高、工艺简单、无污染.     

LG自主创新的SWOT分析及其模式选择

自主创新是支撑企业崛起的有力工具,也是企业持续发展的不竭动力。通过对LG的自主创新情况进行SWOT分析,了解了LG在自主创新方面具备的优势和劣势,明确了其面临的威胁,找到了发展的机遇,并在此基础上为LG自主创新模式的选择与实施提出了建议。     

新旧建筑物间基础沉降缝处理一例

根据工程实例， 提出一种解决新旧建筑物间基础沉降缝的处理方法———架桥法．     

4-甲基-5-烯-庚腈氧化物分子内环加成反应的立体选择性

用过渡状态理论和量子化学AM1方法,对4-甲基-5-烯-庚腈氧化物环加成反应机理进行了研究.结果表明,虽然存在得到2种产物的平行反应,但由于2个反应的速率常数比很大,基本上只得到单一的反式产物.4-甲基-5-烯-庚腈氧化物立体专一选择性不仅由活化焓决定,还与活化熵有关.     

数字信号处理方法在FDTD中的应用

在用ＦＤＴＤ法模拟复杂微波结构时，引入数字信号处理中的Ｍａｐｌｅ算法，以少量时域输出信号为样本，进行参数估计，并预测以后的输出信号波形和频谱，节约７０％的计算时间，模拟结果与未利用数字信号处理的结果相比，有很大改善。