Fractalkine基因真核表达质粒的构建及其在小鼠肝癌细胞中的表达

为克隆小鼠趋化因子Fractalkine(．FK)基因，构建真核表达质粒，并在小鼠肝癌细胞中表达，用以进行肿瘤的基因治疗，用RT-PCR法，从小鼠乳腺癌细胞D2F2扩增FK的cDNA，插入pCR2．1 TOPO载体，测序证实后，将其亚克隆至质粒pIRES中构建FK真核表达载体；用脂质体将重组质粒转染小鼠肝癌MM45 T．Li细胞，经G418筛选获得抗性细胞克隆，用RT-PCR和免疫化学方法鉴定转染细胞中FK基因的表达．结果表明：经限制性内切酶酶切图谱分析和DNA序列测定证实目的基因已插入重组质粒，RT-PCR和免疫化学方法证明转基因MM45T．Li细胞克隆中存在小鼠FK基因的表达。     

landscape Changes in Wenchuan—A Mountainous County in Sichuan, China

Based on the collection of land use maps of three repre sentative years (1972, 1986 and 1995) with a scale of 1 = 100 000, which were derived from interpreted aerial photographs and field surveys, landscape pattern were studied in Wenchuan County of Aba Prefecture, northwest of Sichuan Province, China, Six land use categories were classified according to the actuality of the study area and digitized by manual with the assistance of Geographic Information System (GIS). Landscape spatial and temporal changes were analyzed quanti ficationally by a series of structure, shape, pattern indices, which indicated that grassland dominates in number of patch while both grassland and shrub-land dominate in total area of patch over time. During the last 23 years, the fractal dimension of grassland and shrub-land were decreased while the other types were increased. Highest dominance and fragmentation and lowest diversity characterized the landscape of 1972. In 1986, the landscape pattern had the highest diversity and lowest dominance and fragmentation, which is contrary to the pattern in 1972, Slight change of diversity, dominance and fragmentation has been displayed from 1986 to 1995.     

糖尿病血管内皮细胞功能异常及其机理

糖尿病血管病变（Diabetic vasculopathy）是糖尿病患者致残死亡的首要原因，多种因素参与了这一病理过程，其中血管内皮细胞功能异常起重要作用。随着糖尿病患者血糖升高，患者血管内皮功能受损逐渐加重，与脂代谢紊乱、胰岛素抵抗、氧化应激等多种因素相互影响。认识血管功能的异常，探索其发病机理，对有效预防糖尿病大血管病变，提高患者生存率及生存质量是十分重要的。     

面向动态联盟的产品设计策略研究

在进行动态联盟供求节点分类的基础上，给出了一种动态联盟的结构模型，提出了一个基于动态联盟结构模型的产品结构设计过程与动态联盟结构规划过程相互交叉的产品设计方法。     

高校内部MIS的集成与信息交换

简要介绍了高校内部MIS的现状；指出异构数据库是客观存在的历史问题，认为如何实现异构数据库之间的透明访问是信息交换和系统集成的关键；总结了异构数据库互操作的常用方法以及它们的工作原理，并提出了一种基于XML的解决方案。     

射流悬浮床气固两相流动的数值模拟

从多相流多流体模型、气相湍流k-ε模型与颗粒湍流k_p模型出发,对底部向上射流悬浮床内气固两相流动进行数值模拟,得到了不同入口气速下各截面的颗粒相速度场、颗粒相脉动速度场的分布,结果与实验定性一致。计算结果与实验值对比的结果表明,数值模拟对进一步的研究有指导意义。数值结果与实测值间存在的差异则有待于进行直接三维流动模拟、进一步考察更为合理的边界条件及应用更先进的模型来解决。     

一种解决噪声主动控制中时滞问题的思路

对噪声主动控制进行了探讨，提出一种利用计算机存储概念和寻址技术，从计算机数据处理系统入手解决时滞问题的思路，并给出了结构图。     

多孔陶粒结晶床除磷试验研究

实验以多孔陶粒为载体，在一定条件下，利用非均相及二次成核作用在陶粒表面沉积一层羟基磷酸钙晶体，从而培养出初级晶种．实验将粒径为1．25mm多孔陶粒与石英砂相对比，结果表明采用多孔陶粒作载体形成结晶体效果较好．用该晶种的连续流固定床除磷，获得令人满意的效果．当原水含磷质量浓度在2～5mg/L范围内时，空床线速不大于2．5m/h，水力停留时间不小千12min时, 脱磷固定床除磷率较高且稳定，磷去除率可达90％以上．     

智能化装载机载重检测设计

通过对装载机作业时的转矩进行分析,适当简化相关参数,建立起一个基本的动力学模型.根据这个模型,采用单片机80C196KB,设计了装载机载重量检测的实现方案.根据装载机故障信息的自身特点,基于GSM(global standard for mobile communications)模块TC35,实现装载机与远程控制中心之间的双向无线通讯.最后给出相关的软件设计,主要分析了其中几段关键的程序流程.通过上述软硬件的设计,本方案实现了对装载机载重量的实时检测,并对过载等故障信息进行处理.由此显著地提高了装载机的可靠性和稳定性.     

Emergent Property in Macromolecular Motion

In this paper, the model of inverse cascade fractal super-blocks along one direction (in the positive or negative) in the 3-dimensional space is developed to describe the self-similar motion in macromolecular system. Microscopically the cohesive and dispersed states of the motion blocks are co- existent states with vastly different probability of occurrence. Experimental results and theoretical analysis show that the microscopic cohesive state energy and dispersed state energy of each motion block are respectively equal to the macroscopic glassy state energy kTg and molten state energy kTm of the system. This singularity unveils topologically the non-integrability, mathematically the anholonomy, and macroscopically the emergent property. This singularity also reveals that the glass, viscoelastic and melt states are three distinct emergent properties of macromolecular motion from a macroscopic viewpoint. The fractal concept of excluded volume is introduced to depict the random motion at various scales in