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1.
娜托百合的组织培养和离体快繁   总被引:2,自引:0,他引:2  
以娜托百合的鳞茎及幼茎为外植体成功获得再生植株,并建立了快速无性繁殖系.外植体的最佳芽诱导培养基是MS 0 2~0 5mg/LNAA 0 05~0 8mg/LBA;MS 0 2~0 5mg/LNAA 0 8~2 0mg/LKT;芽的最佳增殖培养基为MS 0 05~0 4mg/LIAA 0 05~0 4mg/LKT;MS 0 1~0 5mg/LBA 0 05~0 5mg/LNAA;芽的最佳增壮培养基和根诱导最佳培养基为MS 0 05~0 8mg/LNAA 0 05~1 0mg/LKT.  相似文献   

2.
泰伯百合的离体快繁   总被引:6,自引:0,他引:6  
以泰伯百合(Liliumtiber)鳞茎、茎尖和带叶芽的茎段为外植体,对其进行组织培养,探讨了激素对芽诱导及增殖的影响,其最佳诱导培养基分别为:MS 0 5mg/LBA 0 05~0 5mg/LNAA;MS 2 0mg/LBA 0 05mg/LNAA;MS 0 1mg/LBA 0 1mg/LNAA.最佳芽增殖培养基为:MS 0 05~0 2mg/LKT 0 02mg/LIAA(或0 05mg/LNAA).生根培养基为:MS 0 2mg/LKT 0 5mg/LNAA.  相似文献   

3.
兰州百合和野百合组织培养及快速繁殖研究   总被引:42,自引:1,他引:42  
以兰州百合(Lilium davidii Var.Unicolor (Hoog)Cotton)和野百合(Lilium brownii F.E.Brown ex Miellez) 鳞茎及叶片为外植体获得再生植株,并建立起快速无性繁殖系。兰州百合鳞茎的最佳诱导培养基为MS+0.6-1.0mg/LBA+0.2mg/LNAA;芽增殖培养基选MS+0.5-0.6mg/LBA 0.1mg/L NAA;生根培养基为1/2MS+0.2mg/l IAA 0.1%活性炭。野百合鳞茎及叶的最佳诱导培养基分别为MS+0.1-0.5mg/LBA 0.1-0.2mg/L NAA或0.5mg/L IAA和MS+0.7mg/L BA 0.07mg/L NAA;鳞茎诱导的芽的增殖培养基为MS+0.2-0.4mg/L BA 0.1mg/L NAA;生根培养基为1/2MS+0.15mg/L NAA 0.1%活性炭。  相似文献   

4.
宜兴百合的组织培养和快速繁殖   总被引:4,自引:0,他引:4       下载免费PDF全文
以组织培养获得的宜兴百合小鳞茎为试验材料,研究了不同基本培养基、NAA与6-BA的配比、矮壮素(CCC)、2,4-D、活性炭、蔗糖及悬浮培养方法对小鳞茎增殖和膨大的影响.结果表明,最佳诱导培养基为MS培养基,有利于提高分化率的最佳配方为:MS+0.15mg/LNAA+2.0mg/L6-BA,小鳞茎膨大增重的最佳培养基为:MS+0.15mg/LNAA+2.0mg/L6-BA+0.1mg/L CCC+6—8%蔗糖,培养方式为液体振荡培养优于固体培养.  相似文献   

5.
兰州百合的组织培养   总被引:4,自引:0,他引:4  
以兰州百合鳞片作为外植体进行组织培养,分别对其进行芽诱导、增殖、生根培养,得到了兰州百合鳞片组织培养的最佳培养基配方:(1)芽诱导培养基:MS+BA0.6mg/L+NAA0.2mg/L+琼脂7.3g/L+蔗糖30g/L,pH5.8;(2)增殖培养基:Ms+BA0.8mg/L+NAA0.05mg/L+琼脂7.3g/L+蔗糖30g/L,pH5.8;(3)生根培养基:MS+BA0.05mg/L+NAA0.8mg/L+琼脂7.3g/L+蔗糖30g/L,pH5.8.观察与分析结果表明,外植体在前两种培养基上的繁殖速率较前人快约15d,第3种培养基与前人的相当,比其快2d.  相似文献   

6.
以兰州百合鳞片作为外植体进行组织培养,分别对其进行芽诱导、增殖、生根培养,得到了兰州百合鳞片组织培养的最佳培养基配方:(1)芽诱导培养基:MS+BA0.6mg/L+NAA0.2mg/L+琼脂7.3g/L+蔗糖30g/L,pH5.8;(2)增殖培养基:Ms+BA0.8mg/L+NAA0.05mg/L+琼脂7.3g/L+蔗糖30g/L,pH5.8;(3)生根培养基:MS+BA0.05mg/L+NAA0.8mg/L+琼脂7.3g/L+蔗糖30g/L,pH5.8.观察与分析结果表明,外植体在前两种培养基上的繁殖速率较前人快约15d,第3种培养基与前人的相当,比其快2d.  相似文献   

7.
以杭白菊茎尖为试材,将其接种在培养基上(以NAA,AC,2,4-D,6-BA及水解乳蛋白为5因素进行正交,设计16组培养基)进行愈伤诱导及出芽,探讨不同添加剂对杭白菊组织培养的影响,同时分析不同因素之间影响的显著性.结果表明,不同种类浓度配比对杭白菊组织培养会产生较大影响,最佳愈伤培养基组成为MS+3mg/L6-BA+0.2mg/LNAA+0.8mg/L2,4-D+0.5%AC+0.2%水解乳蛋白+0.8%琼脂+3%蔗糖;出芽培养基最佳组成为MS+1mg/L6-BA+0.5%AC+0.8%琼脂+3%蔗糖.  相似文献   

8.
本文以长春花无菌苗的芽为外植体进行组织培养。适合芽诱导和增殖的培养基为MS+0.50mg/L6-BA+0.06mg/LIBA+300g/L蔗糖+7g/L琼脂;适合生根的培养基为1/2MS,0.10mg/LIBA+0.20mg/LNAA+30g/L蔗糖+7g/L琼脂。经生根壮苗培养和炼苗25d后,移栽成活率高达92%。  相似文献   

9.
新铁炮百合组织培养和快速繁殖研究   总被引:39,自引:4,他引:35  
以日本新铁炮百合鳞茎及叶为外植体成功获得再生植株,并建立了快速无性繁殖系。鳞片和叶的最佳诱导培养基分别为MS+0.4-1mg/L BA 0.2mg/L NAA;MS 0.1mg/L(或0.5mg/L)BA+0.1mg/L NAA或0.5mg/L IBA。芽增殖培养基选MS+0.2-0.5mg/L BA 0.1mg/L NAA或0.2mg/L IBA。生根培养基为1/2MS+0.1mg/L IBA 0.1%活性炭。  相似文献   

10.
东方百合组织培养和快速繁殖研究   总被引:39,自引:0,他引:39  
以元帅百合 (Acapulco)的盘茎为外植体成功获得再生植株 ,并建立快速无性繁殖系 .鳞片的最佳芽诱导培养基是MS 0 2~ 0 8mg/LKT 0 0 5~ 0 5 0mg/LNAA ;芽增殖最佳培养基是MS 0 10~ 0 5 0mg/LIAA ;根诱导最佳培养基为 12 MS 0 0 5~ 0 10mg/LNAA ;最佳移栽基质是腐殖土、珍珠盐、蛭石粉的配比基质 .  相似文献   

11.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

12.
AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…  相似文献   

13.
本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。  相似文献   

14.
Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with  相似文献   

15.
The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to  相似文献   

16.
17.
The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.  相似文献   

18.
R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).  相似文献   

19.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

20.
The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.  相似文献   

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