一种快速筛查和确定保健品中11种降糖类药物的检测方法

采用高效液相色谱一离子阱飞行时间串联高分辨质谱对于保健品中降糖类药物共11种成分进行快速筛查、定性识别和准确定量。样品经含甲醇溶液超声提取,提取液经OuEChERS吸附剂净化,以C18色谱柱（150mm×2．0mm,3．0μm）分离,5mmol／I。乙酸铵溶液和0．5％甲酸乙腈为流动相梯度洗脱,正离子模式扫描。结果表明,11种化合物在2～1000μg／L范围内呈良好的线性相关性,相关系数均大于0．991。保健品样品中各化合物的定量限（以信噪比≥10计）分别为2．0～5％g／kg。在低、中、高3个添加水平下,各化合物的平均回收率为72．3％～118．1％,相对标准偏差（RSD）为0．5％～8．4％。该方法利用精确质量数匹配和自建标准谱库检索,实现快速筛查,并使用多级特征碎片离子进行确证,具有简便、快速、高效、准确等优点,适用于保健食品中降压降糖类药物的快速筛查和测定。     

芦荟甙—硼砂荧光体系及芦荟甙的测定

采用薄层分离和荧光光度法对芦荟中芦荟甙的含量进行了测定，在硼砂介质中，芦荟甙与硼砂发生化学反应，使体系荧光强度提高了10倍．方法的检测限为2．0×10－9mol·L－1．灵敏度高，重现性好．用标准加入法测定2种样品，回收率在92．9％-104．0％之间．     

超声辅助萃取-高效液相色谱荧光检测法测定贻贝中的多环芳烃

建立了超声波辅助萃取,高效液相色谱荧光检测法测定贻贝中6种多环芳烃化合物的多残留分析方法。对荧光检测条件、色谱分离条件、萃取方式、净化条件进行了优化。在优化条件下,6种多环芳烃化合物实现良好分离,在0．002～0．5mg／L范围内呈良好线性关系,线性相关系数为0．9985-0．9995,检出限为1．0-2．0μg／kg,回收率为78％-92％,相对标准偏差为2．8％～6．3％（n=6）。结果表明：建立的超声辅助萃取-高效液相色谱荧光检测法样品前处理简单,重现性好,回收率高,适合贻贝中多环芳烃的检测。     

衍生荧光法测定蔬菜样品中痕量叶酸含量的研究和应用

本工作比较了测定叶酸的三种荧光分析体系方法的分析性能和应用，详细研究了叶酸内在荧光，KMnO4-光照和H2O2-光照荧光体系．结果表明，叶酸内在荧光较弱，而用衍生方法进行测定，灵敏度可以提高2～3个数量级,在研究的三种体系中，KMnO4-光照法具有适宜的灵敏度和较宽的线性范围，可用于蔬菜样品中叶酸的测定，标准回收率在92．5％，95．0％之间，测定结果令人满意．     

S对As胁迫下桐花树幼苗巯基化合物含量的影响

本研究以红树植物桐花树(Aegiceras corniculatum(L.)Blanco)为实验材料,通过栽培试验研究硫(S)对砷(As)胁迫下桐花树幼苗巯基化合物含量的影响.As含量设为-As(0mg/kg)和+As(60mg/kg)2个处理,而S含量设为-S(0g/kg)、+S(1g/kg)和+HS(2g/kg)3个处理.结果表明:在-As处理组中,桐花树幼苗根、叶中非蛋白巯基(NPT)、谷胱甘肽(GSH)和植物络合素(PCs)等巯基化合物的含量均随着S的施入而有显著升高(p0.05);+As处理组桐花树幼苗根、叶中NPT和PCs含量在-S、+S、+HS处理下均要显著高于-As处理组(p0.05);在+As处理组中,桐花树幼苗根、叶中的NPT和叶中PCs含量随着S的施入显著升高(p0.05),且根中PCs含量与As含量呈显著正相关(r=0.82,p0.05).上述结果说明As的胁迫和S的施入均能促进巯基化合物的合成,显著提高As胁迫下的桐花树幼苗根、叶中巯基化合物特别是PCs的含量.S在红树植物As抗性中具有重要作用,在As污染下的红树林湿地中施入S可以通过巯基化合物代谢提高红树植物对As的抗性.     

毛织物中防虫蛀整理剂残留量的GC/MS测定

将织物试样采用1∶1丙酮－正己烷混合溶剂充分浸渍后进行超声提取，提取液经Celite层析柱净化，40℃旋转蒸发浓缩、定容，由色－质联用仪测定试样中的DCB、Naphthalene、PCP及Parmethrin含量。本方法适合毛织物中防虫柱整理剂、防霉剂和及驱虫制剂残留的定性定量分析，方法对目标化合物的检测限量（LOQs）在0．1－0．5mg/kg之范围内，平均回收率介于89．2％－96．4％之间，相对标准偏差为2．3％－8．1％。     

原子荧光光谱法测定汞、铅、锑的研究

建立了巯基葡聚糖凝胶分离富集-氢化物发生原子荧光法，用于测定汞、铅、锑的含量．采用微波消解样品，以巯基葡聚糖凝胶在不同的pH条件下分离富集汞、铅、锑，再以不同浓度的HCl洗脱后氢化物发生原子荧光光谱法测定含量．汞、铅、锑的线性检测范围分别为0～80，0～150，0～200／μg／L，方法检出限分别为0．05，0．30，0．25μg／L，样品加标回收率汞为99．0％～105．5％、铅为98．0％～101．5％、锑为99．8％～100．6％．本方法具有基体干扰小，灵敏度高，检出限低，线性范围宽的特点，应用于实际样品的测定，结果满意．     

茜草中提取总黄酮实验微型法与常规法的比较

建立茜草提取液中总黄酮含量的微型实验测定方法．对比常规实验和微型实验两种提取方法。利用紫外分光光度法,以芦丁为对照品制定标准曲线,进行样品含量和回收率的测定．其结果为最大吸收波长506nm,线性回归方程为A=-0．01587＋1．0434C（mg／10mL）（R=0．999782）,常量法提取液中总黄酮的平均含量为1．93％,平均回收率为102．74％．微型法提取液中总黄酮的平均含量为1．81％,平均回收率为101．59％．结果表明微型实验方法简便,准确可靠,测定茜草中总黄酮的含量其方法误差与常规实验方法一样在允许范围之内．     

锌钙特口服液中钙锌含量的测定

用原子吸收光谱（AAS）法同时测定锌钙特口服液中钙，锌的含量，为了消除干扰，锌的测定采用标准加入法，平均回收率为99．6％，RSD为0．6％，钙的平均回收率为101．1％，RSD为0．3％，结果显示该方法简便，快速，准确，可用于该类型制剂的含量测定及质量控制。     

一种西夫碱化合物的合成及其在汞测定中的应用

合成一种新西夫碱2-氨基-5-巯基-1,3,4-噻二唑水杨亚胺(MTYMP),用常规法对其结构进行表征.该化合物在300 nm激发下,在410 nm处有强荧光峰,与汞离子可形成稳定的配合物而使其荧光猝灭.据此,该化合物可作为测定汞的荧光试剂,建立一个荧光猝灭测定微量汞的新方法,汞离子的含量在4.0~100.0μg·L-1范围内与试剂的荧光熄灭值呈线性关系,方法的检出限为0.315μg·L-1.该方法应用于废水中微量汞的测定,结果满意.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.     

Tracking data management using topology of moving object database system for moving object constrained trajectory in LBS application

Advances in wireless technologies and positioning technologies and spread of wireless devices, an interest in LBS (Location Based Service) is arising. To provide location based service, tracking data should have been stored in moving object database management system (called MODBMS) with proper policies and managed efficiently. So the methods which acquire the location information at regular time intervals then, store and manage have been studied. In this paper, we suggest tracking data management techniques using topology that is corresponding to the moving path of moving object. In our techniques, we update the MODBMS when moving object arrived at a street intersection or a curved road which is represented as the node in topology and predict the location at past and future with attribute of topology and linear function. In this technique, location data that are corresponding to the node in topology are stored, thus reduce the number of update and amount of data. Also in case predicting the location,because topology are used as well as existing location information, accuracy for prediction is increased than applying linear function or spline function.