南丰蜜橘组织培养及植株再生

以南丰蜜橘种子无菌苗的子叶、上胚轴、茎端、下胚轴为材料,在不同激素配比的MS基本培养基上进行外植体筛选和诱导不定芽分化、继代及生根培养,确定了诱导植株再生的最佳外植体和最适条件:(1)诱导不定芽分化最好外植体为上胚轴,不定芽分化率达90%;(2)继代培养中最适激素配比为MS+0.8 mg/L 6-BA+0.1 mg/L NAA+0.5 mg/LD-泛酸钙;(3)生根最适合培养基为MS/2+0.1 mg/L NAA.     

辣椒离体高效再生体系及其卡那霉素筛选体系的建立

选用辣椒(CapsicumannuumL.)4个品种的子叶以及下胚轴为外植体进行不定芽的诱导分化、生长及生根成苗试验.在组织培养的各个阶段的培养基中,附加不同种类及不同质量浓度的激素组合,观察测量外植体在各种培养基中的生长情况,从中筛选出最优的激素配比培养基,从而建立了辣椒的高效离体植株再生体系.在此基础上,将不同质量浓度梯度的卡那霉素(Kan)分别加入子叶、下胚轴愈伤组织诱导及不定芽分化阶段的培养基中,观测Kan对外植体生长的影响,以确定各阶段的最低致死质量浓度,用于辣椒的遗传转化研究.试验结果表明最佳的辣椒离体再生培养基为:芽分化阶段,MS+6-BA3.0mg·L-1+IAA0.1mg·L-1;不定芽伸长阶段,MS+GA32mg·L-1+6-BA0.5mg·L-1;诱导生根,MS+IAA1.0mg·L-1.合适的Kan质量浓度为:下胚轴出愈为80mg·L-1;子叶分化出不定芽为60mg·L-1.     

厚皮甜瓜黄河蜜植株再生研究

利用厚皮甜瓜黄河蜜3号子叶及下胚轴进行植株再生实验研究.结果表明:子叶不定芽分化率高于下胚轴,适合子叶不定芽分化的培养基为MS 6-BA1.5 mg/L NAA0.2 mg/L,分化率为60%,每个外植体平均分化芽数9.4个.适合下胚轴不定芽分化的培养基是MS 6-BA 1.75 mg/L NAA0.1 mg/L,分化率为9%,每个外植体平均分化芽数8.5个.对不定芽黑暗处理2天后用1/2 MS IAA0.2 mg/L诱导生根,生根率达91.12%,平均根数为12条.200 mg/L的NAA对无根苗处理20 min后移入1/2河沙 1/2蛭石基质中,1/2 MS营养液浇灌,试管外生根率为52%.     

苜蓿不同品种愈伤组织诱导、分化和再生能力的比较研究

以新疆大叶、甘农1号、甘农3号和陇东4个苜蓿品种的下胚轴、子叶和子叶节为外植体,比较了不同苜蓿品种及外植体愈伤组织诱导、分化及再生能力的差异,研究了不同浓度及配比的植物生长调节物质对愈伤组织诱导和芽分化再生的影响。结果表明,4个苜蓿品种再生能力依次为新疆大叶>甘农3号>甘农1号>陇东苜蓿,下胚轴外植体在愈伤诱导培养基(MS 2mg/L2,4-D 0.2mg/LZT)上出愈率分别为94%,83%,71%和58%,胚性愈伤组织在分化培养基(MS 0.2mg/LZT 50mg/L谷氨酰胺)上分化率分别为15.8%,9.2%,7.1%和4.5%;下胚轴再生能力优于子叶节和子叶;丛生芽在生根培养基(1/2MS)上生成完整的根;各品种的再生苜蓿植株均成功移栽到土壤中,成活率100%。上述4个苜蓿品种分化再生能力的阶段性研究结果为今后通过基因工程方法改良苜蓿奠定了基础。     

辣椒茎尖离体培养及植株再生

以4个辣椒品种茎尖为外植体，在MS 4mg／L BA 1mg／L IAA 4mg／L AgNO3培养基上诱导不定芽分化，在MS 2mg／L BA 0．1mg／L IAA，MS 0．5mg／L BA 0．1mg／L IAA诱导不定芽茎伸长．结果，平均不定芽诱导率达85％，平均不定芽茎伸长率可达110％，再生植株频率达75％左右．初步实验结果表明：与子叶及下胚轴培养相比，茎尖培养具有成苗率高、基因型不敏感、再生周期短等优势，是解决辣椒组织培养不定芽茎伸长困难，提高再生植株诱导频率的有效途径．     

影响花生下胚轴丛生芽再生因素的研究

以花育22号成熟种子萌发后的下胚轴为外植体，分析了激素配比、外植体萌发日龄、AgNO3、NAA以及不同基因型对花生下胚轴植株再生的影响．结果发现，MS＋6-BA（12mg/L）＋NAA（0．8mg/L）的组合对下胚轴芽的分化较好，萌发4～5d的外植体用于丛生芽诱导较佳，3mg／L的AgNO3可提高下胚轴芽的分化率并有效减轻下胚轴的褐化，1mg/L的NAA利于组培苗生根，组培苗移栽后能正常开花、结实．不同基因型花生的芽诱导率存在差异．     

黄瓜子叶节离体再生体系的建立

以MS为基本培养基,以黄瓜子叶节为外植体,设置了不同激素浓度,直接诱导出不定芽,建立了黄瓜的离体再生体系.结果表明,最佳芽诱导培养基为MS+BA 1.0 mg/L+IAA 0.1 mg/L+ABA 1.0 mg/L+AgNO3 2.0 mg/L,平均每外植体可诱导出2.9个芽;最佳芽伸长培养基为MS+KT 0.5 mg/L;1/2 MS最适于黄瓜的生根.     

特种工业用油料作物Lesquerella fendleri的愈伤组织诱导和植株再生

以Lesquerella fendleri下胚轴和子叶作为外植体,研究了不同的激素浓度和组合对于愈伤组织诱导和植株再生的影响．研究表明,L．fendleri下胚轴和子叶在许多激素组合的培养基上都能诱导出愈伤组织．当6-BA的浓度从0提高到1．0mg．L^-1,下胚轴的愈伤组织诱导频率也从35．14％提高到97．30％,进一步提高6-BA浓度会降低下胚轴的愈伤组织诱导频率;将NAA浓度从0提高到0．2mg．L^-1,下胚轴的愈伤组织诱导频率从70％提高到76．76％,进一步提高NAA浓度则降低下胚轴的愈伤组织诱导频率;将2,4-D的浓度从0提高到0．5mg．L^-1,下胚轴的愈伤组织诱导频率从64．29％提高到85．00％,进一步提高2,4-D浓度会降低下胚轴的愈伤组织诱导频率．当6-BA的浓度为0．5mg．L-时子叶的愈伤组织诱导频率最高,达到80．00％;提高NAA浓度可以提高子叶愈伤组织诱导频率,提高2,4-D浓度则会降低子叶的愈伤组织诱导频率．绝大多数愈伤组织在含有6-BA和NAA的MS培养基上都能够分化出许多不定芽．不定芽在MS基本培养基能够诱导生根,生根率在80％以上．     

发根农杆菌对苦马豆转化及植株再生的初步研究

用发根农杆菌A4转化苦马豆子叶和下胚轴外植体，获得转化发根．将发根转移到MS无激素培养基上，发根能够持续生长，并于继代40天后自发形成不定芽．将不定芽转移到1／2MS无激素培养基，长出不定根，形成再生植株．再生植株表现出典型的发根综合症，初步判断再生植株为转化植株．     

唐古特白刺愈伤组织诱导及再生体系的研究

以唐古特白刺(Nitraria tangutorum Bobr.)子叶、下胚轴、根为外植体, 接种在不同浓度激素配比的培养基中, 研究愈伤组织的诱导及植株再生.结果表明: 子叶和下胚轴是诱导愈伤组织的良好外植体; 以MS补加2,4-D(0.5～1.0 mg·L-1)或6-BA(1.0～2.0 mg·L-1)+NAA(0.5～1.0 mg·L-1)可100%高频产生愈伤组织;在MS+6-BA 2.0 mg·L-1+NAA 0.5 mg·L-1培养基上,下胚轴可产生不定芽; 最佳增殖培养基为MS+6-BA 0.5 mg·L-1+NAA(0.5～1.0 mg·L-1),增殖系数为5.75; 最佳生根培养基为1/2 MS+IBA 0.5 mg·L-1,生根率达94.6 %.     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

Perspherical Approaches for Electrons in Atoms and Charged Excitons in Quantum Wells

Instead of following Fock’s expansion,we solve the Schrodinger equation for some quantum mechanical manybody systems such as electrons in atoms and charged excitons in quantum wells in a similar way in hyperspherical coordinates by expanding the wave functions into orthonormal complete basis sets of the hyperspherical hannonics(HHs)of hyperangles and generalized Laguerre polynomials(GLPs)of the hyperradius.This leads the equation to     

Advances in broadband wireless communications under high-mobility scenarios

Future mobile communication systems aim at providing very high data transmission rates, even in high-mobility scenarios such as high-speed wheel-track trains, maglev trains, highway vehicles, airplanes, guided missiles or spacecraft. A particularly important commercial application is the strong and increasing worldwide demand for high- speed broadband wireless communications （up to 574.8 km/ h test speeds or 380 km/h commercial speeds） in railways, providing data, voice and video services for applications such as onboard entertainment services to passengers, train control, train dispatch, train sensor status handling and sur- veillance. In such high-mobility scenarios, there are a number of communication challenges, including fast hand- over, location updating, high-speed channel modeling, estimation and equalization, anti-Doppler spreading tech- niques, fast power control, and dedicated network architec- ture. Because signal transmission in very high-speed scenarios will inevitably experience serious deterioration, it is imperative to develop key broadband mobile communi- cation techniques for such high-speed vehicles.     

Wuhan University Journal of Natural Sciences TOTAL CONTENTS

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海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Scale-based spatial data model for GIS

Being the primary media of geographical information and the elementary objects manipulated, almost all of maps adopt the layer-based model to represent geographic information in the existent GIS. However, it is difficult to extend the map represented in layer-based model. Furthermore, in Web-Based GIS, It is slow to transmit the spatial data for map viewing. In this paper, for solving the questions above, we have proposed a new method for representing the spatial data. That is scale-based model. In this model we represent maps in three levels: scale-view, block, and spatial object, and organize the maps in a set of map layers, named Scale-View, which associates some given scales.Lastly, a prototype Web-Based GIS using the proposed spatial data representation is described briefly.     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to