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1.
真盐生植物盐地碱蓬茎尖组织培养和植株再生   总被引:1,自引:0,他引:1  
以盐地碱蓬茎尖为外植体,探讨了不同激素浓度配比对植株再生的影响.结果表明,以盐地碱蓬茎尖为外植体,在附加1.0mg/L6-BA和0.5mg/L IAA的MS培养基上培养30天可诱导出不定芽,不定芽的诱导频率达到100%以上,每个外植体的平均出芽数达到3.63,60天后形成丛生芽.不定芽转至MS培养基中培养10天生根形成完整植株.本文建立了真盐生植物盐地碱蓬高效的再生体系,为遗传转化体系的创建奠定了基础.  相似文献   

2.
福建省山樱花的组织培养及植株再生   总被引:5,自引:0,他引:5  
以春季萌发的福建山樱花嫩枝为外植体诱导丛生芽 。在1/4MS+BA1.0mg/L IBA0.1mg/L的培养基上,其丛生芽诱导率达87.5%;在继代培养中选择MS+BA1.0mg/L IBA0.1mg/L GA30.3mg/L或KT1.0mg/L NAA0.1mg/L BA30.3mg/L培养基,不定芽增殖较快。赤霉素对其不定芽的伸长有明显效果,当芽伸长在3-4cm时,切下置于生根培养基1/2MS+NAA1.0mg/L IBA1.0mg/L BA0.75mg/L中,生根率达90%以上,移栽成活率达95%。  相似文献   

3.
新疆杨植株再生体系的建立   总被引:16,自引:1,他引:16  
从培养基筛选、激素调控等方面探索建立新疆杨高效植株再生体系。结果表明,不同种类的细胞分裂素影响叶盘不定芽分化频率,其中较低浓度的TDZ(Thidiazuron)可显提高叶盘不定芽分化频率,生长素的种类与浓度对叶盘不定芽分化影响不显。较适合新疆杨不定芽分化的培养基为:1/2MS TDZ0.005mg/L BA0.25mg/L IAA0.5mg/L;壮苗培养基为:MS BA0.2mg/L IAA0.2mg/L;生根培养基为:1/2MS。在此条件下,不定芽分化频率可达100%。  相似文献   

4.
以茉莉的下胚轴为外植体,研究了茉莉直接不定芽器官发生途径的再生体系.结果表明:以MS为基本培养基,附加0.5mg/L BAP和0.01mg/L NAA的诱导效果最佳,其不定芽再生率高达92%,外植体平均不定芽数目为4.2.适宜不定芽增殖的最佳培养体系为MS+1.0mg/L BAP+0.1mg/L NAA.1/2MS+0.5mg/LIBA+0.5mg/L IAA+100mg/L适于再生幼茎的生根,生根率达91%,生根后经移栽成活.  相似文献   

5.
香石竹茎段无性系的建立   总被引:2,自引:0,他引:2  
以香石竹无菌茎段为外植体成功获得再生植株,并建立快速无性繁殖系,茎段愈伤组织的诱导及芽点的分化最佳培养基是1/2MS BA1 2,4mg/L-D0.4mg/L,芽增殖的最佳培养基是MS BA0.5mg/L NAA0.5mg/L,根诱导最佳培养基是1/2MS IAA0.2mg/L,最佳移栽基质是炉碳渣。  相似文献   

6.
以红甜菜变异植株的嫩茎为外植体成功获得再生植株,并建立快速无性繁殖系.嫩茎愈伤组织的诱导及不定芽分化的理想培养基是MS+BA 1mg/L;不定芽的增殖生长理想培养基为MS+BA0.1mg/L+IAA0~0.5mg/L,理想的移栽基质是炉碳渣和河沙.  相似文献   

7.
凤仙花茎段培养与快速繁殖   总被引:2,自引:0,他引:2  
试验以茎段为外植体,进行凤仙花离体再生与快速繁殖技术研究.结果表明:在附加BA1.0mg/L、NAA0.2mg/L、3%蔗糖的MS固体培养基上可诱导茎段外植体直接产生大量不定芽,诱导率达100%;在含BA1.0mg/L、N从0.2mg/L、3%蔗糖的MS培养基上进行继代培养,可大量增殖;在含NAA、1.0mg/L。3%蔗糖的1/2MS培养基中长根成苗.本试验表明风仙花离体繁殖,增殖系数大,适合于种苗快速繁殖.  相似文献   

8.
叶子花的组织培养及快速繁殖研究   总被引:3,自引:0,他引:3  
以叶子花的茎尖为外植体,通过13种培养基的试验,从中筛出适宜叶子花组织培养、快速繁殖的一整套稳定、高效的生产程序,即芽诱导培养基为MS BA0.2mg/L IAA1.0mg/L,增殖培养为MS BA0.5mg/L IAA1.5mg/L,生根培养基为MS IBA0.1mg/L 2,4,5-T0.1mg/L。为规模化生产叶子花提供了快速繁殖的方法。  相似文献   

9.
利用野生裂叶秋海棠叶片及叶柄培养,通过两种方式获得大量再生植株。(1)直接从外植体表面诱导出不定芽,最佳诱导培养基为MS 6-BA 3mg/L,不定芽在培养基MS NAA0.5mg/L 6-BA0.5mg/L增殖成丛生芽.(2)先诱导出愈伤组织,再由愈伤组织分化出大量不定芽.最佳愈伤组织诱导培养基为MS NAA0.5mg/L 2,4-D0.5mg/L 6-BA0.5mg/L,愈伤组织不定芽分化培养基为MS 6-BA 1mg/L,试管苗在含1/5MS无机盐的培养基上生根,之后移栽成活率达85%。  相似文献   

10.
以猪笼草的幼嫩茎段为外植体进行离体组织培养及植株再生,研究结果表明:以液体培养基1/2MS+6-BA1.0mg/I.+NAA0.1mg/L对芽的初始诱导以及固体培养基1/2MS+6-BA2.0mg/L+NAA0.1mg/L对不定芽的增殖效果最好,增殖率达419%;生根培养基以1/4MS+IBA0.5mg/L+活性碳(0.01%)为最佳,生根率可达100%.  相似文献   

11.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

12.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

13.
AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…  相似文献   

14.
本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。  相似文献   

15.
Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with  相似文献   

16.
The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to  相似文献   

17.
18.
The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.  相似文献   

19.
R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).  相似文献   

20.
The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.  相似文献   

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