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1.
荷木种群在不同群落中的分子生态研究   总被引:11,自引:1,他引:10  
用扩增片段长度多态性(AFLP,amplified fragment length polymorphism)方法对荷木(Schima superba)在3个不同群落中的遗传变异进行了研究。AFLP分析结果表明其遗传多样性较高。用AMOVA(analysis of molecular variance)分析表明,95.99%的遗传变化表现在种群内,4.01%的遗传分化表现在种群间(P〈0.001)  相似文献   

2.
应用RAPD标记构建马尾松单株树遗传连锁图谱   总被引:5,自引:0,他引:5  
利用马尾松(Pinusmassoniana)崇义群体C-16号单株上40粒种子的胚乳为作图群体材料,用随机扩增多态性DNA(RAPD)方法构建马尾松单株遗传连锁图谱,从被步筛选的80个引物中,得到16个具有多态性产物的引物,对这16种引物进行Mendelian1:1分离检测(卡方检验,P<0.05),我们得到符合Mendelian1:1分离比例的RAPDs标记49个.通过对49个标记的连锁关系进行分析,其中29个标记分布在12个连锁群上,总图距为483.3cM,平均图距为28.42cM,20个标记没有连锁到任何连锁群上,需要继续进行大量标记的连续分析,构建完善的高密度的遗传图谱,使之能够对数量性状位点QTLs进行定位,从而进行分子标记辅助选择育种(MAS).  相似文献   

3.
杉木杂种群体分子框架遗传连锁图初报   总被引:19,自引:1,他引:18  
利用随机扩增DNA多态性分子遗传标记-RAPD,以杉木J0和F11两个亲本杂交形成的F1群体为作图群体,从1040个随机引物中筛选出78个引物,对78个F1群体及双亲样本进行了RAPD扩增,共获得129个RAPD标记,首次构建了杉木分子遗传连锁框架图,其中J0亲本包含8个连锁群,标记覆盖的基因组总长度约为595.2cM,F11亲本包含4个连锁群,标记覆盖的基因组总长度约为315.3cM。129个RAPD标记中偏分离标记占14.7%。本图谱为构建饱和的杉木分子遗传图谱提供了框架结构,为进一步开展杉木分子遗传方面的研究奠定了基础。  相似文献   

4.
FabriciusE.M.1)GuschmannM.2)LangfordA.1)HelB.1)WilsnerG.P.3)LaiR.4)VogelM.2)BierJ.1)(1)KlinikfürMund-,Kiefer-undGesichtschiru...  相似文献   

5.
水稻花发育相关MADS-box基因的克隆   总被引:1,自引:1,他引:0  
以水稻幼穗总RNA为模板,利用3′-RACE克隆了8个接近于全长的水稻MADS-box 基因的cDNA.在GenBank 的数据库中查询表明,其中2个FDRMADS1,FDRMADS2分别与已报道的水稻MADS-box 基因Os-MADS5和OsMADS45有极高的同源性,另外6个为新的尚未见报道的水稻MADS-box 基因.系统进化树分析表明FDRMADS1,FDRMADS2和FDRMADS4可能与C组基因的功能相关;FDRMADS3,FDRMADS6和FDR-MADS7可能与A 组基因的功能相关  相似文献   

6.
采用DMD基因的全长CDNN(4kb)为杂交探针,分析了中国人群DMD基因含外显子片段(exon-containingfragment)的BglⅡ限制性片段和RFLPs.新揭示出四个BglII片段,DMD基因全长范围内含外显子的BglⅡ限制性片段总数至少为59个,这为制作DMD基因的BglⅡ部分限制图奠定了重要基础;另分析了四个BglⅡRFLPs在中国人群中的等位频率,在cDNA2b-3、cDNA4-5a和cDNA5b-7三个亚克隆探针的杂交带型中,杂合体频率预期值(EHF)分别是0.33、0.33和0.44,实际观察值为:040、0.40、0.48,表明有较高的临床应用价值.此外,在BglⅡ/2b-3和BglⅡ/4-5a的带型中,还分别发现一个新的罕见等位片段.  相似文献   

7.
rojanA.1)KreuserK.A.2)FluryR.3)SchmidM.4)SchnciderJ.4)SchrderS.5)(1)DepartementfürlnnereMedizin,UniversittspitalZürich;2)Medi...  相似文献   

8.
本文介绍在MicrosoftWINDOWSFORWORKROUPS3.1(以下简称WFW)网络环境下用MicrosoftVISUALBASIC3.0(以下简称VB3.0)实现不同计算机之间动态数据交换的方法,并给出了主要程序  相似文献   

9.
报道了两种4-N,N-二甲氨基偶氮苯-4′-磺酸酯衍生物的合成,获得了其主客及侧链极化聚合物薄膜的d33值(4pm/V,9pm/V);它们分别高于目前研究较多的DR-1/PMMA(主客,2.5pm/V)、CEM-MMA(侧链,5.63pm/V)的对应值,并探讨了其影响因素  相似文献   

10.
由HMnO4和(NH4)6Mo7O24;4H2O合成了标题化合物K0.5(NH4)5.5「MnMo9O32」.6H2O用X-射线测定了晶体结构,该化合物的晶体属三方晶系,空间群R32;a=15.846(4),C=12.396(30)A,Mr=1657.28,V=2695.6A^3,Z=3,Dc=3.82g/cm^3,μ41.5=cm^-1,F(000)=2967和3024个可观测衍射「Fp≥3σ(  相似文献   

11.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

12.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

13.
AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…  相似文献   

14.
本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。  相似文献   

15.
Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with  相似文献   

16.
The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to  相似文献   

17.
18.
The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.  相似文献   

19.
R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).  相似文献   

20.
The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.  相似文献   

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