罗氏沼虾(Macrobrachium rosenbergii)血细胞的分类与组成

目的:对罗氏沼虾的血细胞进行分类,研究各类血细胞的组成情况.方法:从罗氏沼虾心脏取血制血涂片,经瑞氏染色后光镜观察.结果:根据血细胞的形态、核质比、细胞质颗粒的大小与分布以及染色情况,可将罗氏沼虾的血细胞分为:①透明细胞,其胞质中无明显颗粒,核质比大;②半颗粒细胞,其胞质中含有明显的颗粒,颗粒大小不均,分布不均匀,核质比小;③颗粒细胞,其胞质中含有明显的颗粒,颗粒大小均一,分布均匀,核质比小.结论:罗氏沼虾的血细胞可分为透明细胞、半颗粒细胞和颗粒细胞3类,它们在血细胞中所占比例依次为:颗粒细胞<半颗粒细胞<透明细胞.     

利用流式细胞术研究3种贝类的血细胞分类

利用流式细胞术对西施舌(Coelomactra antiquata)、波纹巴非蛤(Paphia undulata)、双线紫蛤(Sanguinolaria diphos)3种双壳贝类的血细胞组成进行分析,结果表明3种贝类血细胞都可分为透明细胞、小颗粒细胞和大颗粒细胞3个亚群.西施舌的血细胞中,透明细胞占11.02%,小颗...     

短蛸血细胞的形态结构、类型、细胞化学特性及其吞噬活性研究

血细胞作为免疫防御的第一道防线，在软体动物的非特异性免疫系统中具有重要作用.为了揭示头足类动物血细胞的形态结构、生物学性质及其免疫学功能，实验利用活体染色、细胞化学和电镜技术等对短蛸血细胞的形态结构、类型、细胞化学性质及其弧菌吞噬活性进行了研究.研究结果显示：短蛸血细胞具有大透明细胞、小透明细胞、小颗粒细胞和大颗粒细胞4种类型.大透明细胞约占血细胞总数22.6%，平均直径为11.64±0.82?μm，胞质中没有或仅含有很少量的颗粒，细胞表面光滑无伪足伸出；小透明细胞约占血细胞总数的1.7%，平均直径为8.88±0.88?μm，胞质中仅含有少量颗粒，细胞核对台盼蓝呈阳性反应，核质比较大；小颗粒细胞约占血细胞总数的50.7%，平均直径为12.82±1.54?μm，胞质中具有许多大小较为均一的嗜碱性小颗粒，有些细胞内还含有小空泡，不为中性红所着色，疑为颗粒脱内容物所致，细胞表面有较短伪足伸出；大颗粒细胞约占血细胞总数的25.20%，平均直径为13.66±1.50?μm，胞质中具有大小不匀的许多嗜碱性颗粒，有些细胞内也含有不为中性红所着色的较大空泡，细胞表面有许多长伪足伸出.颗粒细胞中所含有的大量嗜碱性颗粒可能与蛋白质等分泌物的活跃合成有关.大颗粒细胞还具有全质分泌的特性，小透明细胞极可能是大颗粒细胞全质分泌后的一时性残余胞体.弧菌吞噬实验结果表明，两类透明细胞均没有弧菌吞噬活性，两类颗粒细胞均具有弧菌吞噬活性，暗示这两种颗粒细胞很可能是短蛸发挥细胞免疫功能的关键性细胞，不仅与某些物质的活跃合成与分泌有关，而且可能还直接参与了外来病原的吞噬及清除.研究结果为揭示短蛸血细胞在非特异性免疫防御中的作用奠定了基础，对于丰富软体动物免疫学也具有重要的科学价值.     

细胞松弛素B诱导鲍异源三倍体

用细胞松弛素B(CB)处理受精卵(九孔鲍♀×盘鲍♂),抑制第二极体释放,诱导异源三倍体.在水温24℃下,分别进行了不同起始处理时间(受精后5~25 min)、不同药物处理浓度(0.2~1.0 mg/L)和不同持续处理时间(5~15 min)的实验.结果表明:受精后10~15 min开始处理的效果较好,平均担轮幼虫成活率为52.07%,平均三倍体率为55.81%;最佳药物处理质量浓度为0.4 mg/L,最佳持续处理时间为10 min,担轮幼虫率为21.43%,异源三倍体率为50.96%;药物空白对照组1(九孔鲍♀×九孔鲍♂)担轮幼虫率为85.38%,二倍体率为95.42%;药物空白对照组2(九孔鲍♀×盘鲍♂)担轮幼虫率为54.37%,二倍体率为75.87%.综合考虑,鲍异源三倍体的适宜诱导条件为:在受精后13 min,即当40%~50%受精卵排出第一极体时,用0.4 mg/L的CB处理10 min.     

曼氏无针乌贼血细胞形态及分类

采用不同固定剂和染色剂对曼氏无针乌贼的血细胞进行处理,利用组织学的手段对血细胞形态进行了观察、记数和测量.曼氏无针乌贼血细胞浓度平均为2.5×107 cells/mL.根据细胞质中颗粒的多少、大小、折光性、染色特性及血细胞的大小、核质比等,将曼氏无针乌贼的血细胞分为二大类:透明细胞和颗粒细胞.透明细胞近圆形,直径7.65～8.28 μm,细胞核卵圆形,较大,细胞质中颗粒很少,核质比约0.66,约占总细胞数的45.3%;颗粒细胞圆形,直径9.58～10.42 μm,细胞质中有大量嗜酸性颗粒,核质比较小,约0.40,有些具双核,约占总细胞数的54.7%.研究了曼氏无针乌贼血细胞的形态和分类,为曼氏无针乌贼血细胞免疫的深入研究奠定了基础.     

皱纹盘鲍与九孔鲍杂交的研究

采用皱纹盘鲍与九孔鲍进行杂交,并与两亲本自交进行育苗比较.结果表明:自交组的受精率、孵化率以及存活率显著高于杂交组(P0.05);皱纹盘鲍(♀)×九孔鲍(♂)和皱纹盘鲍(♂)×九孔鲍(♀)杂交的受精率分别为0.9%和3.2%,受精率存在显著差异(P0.05),而孵化率分别为12.4%和13.1%,二者差异不显著(P0.05),二者之间幼体存活率的差异也不显著(P0.05);自交组和杂交组之间的鲍生长速度存在明显的差异(P0.05),杂交组合的生长速度明显比自交组合的要快;但杂交组中稚鲍的存活率和自交组之间差异不显著(P0.05).     

拟环豹蛛血细胞相差显微镜的初步研究

应用相差显微镜 ,研究了拟环豹蛛 Pardosa pseudoannulata (狼蛛科 Lycosidae)的血细胞 ,观察是有原血细胞、浆血细胞、颗粒细胞、类绛色细胞和脂细胞等五种类型 .拟环豹蛛与同目不同科的大腹园蛛 Araneusventricosus(园蛛科 )比较 ,两者分别都具有上述五种血细胞     

3种淡水育珠河蚌血细胞类型的研究

作者以圆背角无齿蚌、三角帆蚌和褶纹冠蚌为材料 ,通过多种染色方法并结合酸性磷酸酶、非特异性酯酶和过氧化物酶等 3种酶对血细胞的显示定位 ,可将 3种淡水育珠蚌的血细胞分为 :颗粒细胞、无颗粒细胞、透明细胞和类淋巴细胞四种类型 .作者认为 ,透明细胞可能是颗粒细胞衰老形成的 .     

2种虾蛄血淋巴细胞的初步研究

采用记数板记数、常规法涂片对口虾蛄和黑斑口虾蛄的血淋巴细胞进行了显微观察和分析。根据血细胞的大小、形态、核质比、细胞质颗粒数,将口虾蛄和黑斑口虾蛄的血细胞进行分类。结果表明,口虾蛄和黑斑口虾蛄的血细胞可分成3种类型:无颗粒细胞、小颗粒细胞和大颗粒细胞,口虾蛄和黑斑口虾蛄的的平均血细胞密度分别为(1.755±1.086)×103/mL、(1.149±0.832)×103/mL。在此基础上比较了2种虾蛄在不同性别条件下,3种血淋巴细胞在循环血细胞总数中所占比例的变化,并与其他甲壳动物进行了比较。     

人工诱导盘鲍异源雌核发育多倍体的初步研究

通过预实验,观察和了解在24.8℃下九孔鲍精子与盘鲍卵子授精,及其受精卵第一极体和第二极体出现的时间.采用不同浓度的乙烯脲失活九孔鲍精子的遗传物质,确定其最佳处理浓度和处理时间.遗传物质失活的九孔鲍精子和盘鲍卵子授精,用CB进行不同持续处理时间抑制受精卵第一极体和第二极体出现的方法诱导多倍体.实验结果表明,在水温24.8℃下,作为失活九孔鲍精子遗传物质的乙烯脲,当其质量分数为0.5‰和处理时间为10 min是比较适宜的;用遗传物质失活的九孔鲍精子与盘鲍卵子授精,在授精后10 min,用浓度为1.0 mg.dm-3的CB持续处理受精卵24 min,表观三倍体率达65%以上,表观四倍体率达5%左右.稚鲍的倍性检查中,雌核发育多倍体占总多倍体数的80%,杂交多倍体占20%.盘鲍异源雌核发育的三倍体率达到93%,四倍体率为3%左右.     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

Perspherical Approaches for Electrons in Atoms and Charged Excitons in Quantum Wells

Instead of following Fock’s expansion,we solve the Schrodinger equation for some quantum mechanical manybody systems such as electrons in atoms and charged excitons in quantum wells in a similar way in hyperspherical coordinates by expanding the wave functions into orthonormal complete basis sets of the hyperspherical hannonics(HHs)of hyperangles and generalized Laguerre polynomials(GLPs)of the hyperradius.This leads the equation to     

Advances in broadband wireless communications under high-mobility scenarios

Future mobile communication systems aim at providing very high data transmission rates, even in high-mobility scenarios such as high-speed wheel-track trains, maglev trains, highway vehicles, airplanes, guided missiles or spacecraft. A particularly important commercial application is the strong and increasing worldwide demand for high- speed broadband wireless communications （up to 574.8 km/ h test speeds or 380 km/h commercial speeds） in railways, providing data, voice and video services for applications such as onboard entertainment services to passengers, train control, train dispatch, train sensor status handling and sur- veillance. In such high-mobility scenarios, there are a number of communication challenges, including fast hand- over, location updating, high-speed channel modeling, estimation and equalization, anti-Doppler spreading tech- niques, fast power control, and dedicated network architec- ture. Because signal transmission in very high-speed scenarios will inevitably experience serious deterioration, it is imperative to develop key broadband mobile communi- cation techniques for such high-speed vehicles.     

Wuhan University Journal of Natural Sciences TOTAL CONTENTS

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海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Scale-based spatial data model for GIS

Being the primary media of geographical information and the elementary objects manipulated, almost all of maps adopt the layer-based model to represent geographic information in the existent GIS. However, it is difficult to extend the map represented in layer-based model. Furthermore, in Web-Based GIS, It is slow to transmit the spatial data for map viewing. In this paper, for solving the questions above, we have proposed a new method for representing the spatial data. That is scale-based model. In this model we represent maps in three levels: scale-view, block, and spatial object, and organize the maps in a set of map layers, named Scale-View, which associates some given scales.Lastly, a prototype Web-Based GIS using the proposed spatial data representation is described briefly.     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to