漆酶对共基质体系下吲哚的去除研究

采用漆酶对低浓度单基质吲哚及与苯酚共存下低浓度吲哚进行去除实验。结果表明,与单基质相比,含有苯酚的体系中,漆酶对吲哚的去除效果更好;漆酶不仅可以同时去除共基质中的苯酚和吲哚,苯酚的存在还促进了漆酶对吲哚的去除;共基质体系中,苯酚浓度与吲哚的去除率有较好的线性相关性,苯酚的中间产物可能与漆酶组成了新的介体系统,促进了吲哚的氧化;与单基质相比,共基质体系中漆酶对吲哚的去除有两个较优的pH值,分别为3.5和6.5,且苯酚的加入可以减小漆酶的用量。     

不同缓冲培养体系下三株白腐菌的生物学特性

研究了 5种不同缓冲培养体系对云芝 (Coriolussp .)S、栓菌 (Trametessp .)CD和平菇 (Pleurotussp .)BP的生长及其调节系统 pH值和产漆酶能力的影响 .结果发现 :pH对这三株白腐菌的生长、改变体系pH能力、漆酶分泌均有显著影响 .这三株白腐菌最适合的生长条件均为中、酸性 ,一定的 pH范围内菌株S具有较强的降低 pH的能力 ,可使培养体系 pH持续降低 ,随后有所回升 ,菌株S不分泌漆酶 ;菌株CD持续抬升体系pH ,可使起始 pH为 3.0左右的培养体系 pH在第 10d升到 8.2 ,其最适合产漆酶的 pH为 3.0 ,第 6d漆酶酶活可达 9.0U/mL ;菌株BP基本不改变体系的 pH ,最适合分泌漆酶的pH为 5 .0 ,第 6d漆酶酶活可达 192 .0U /mL .     

白腐菌产漆酶的发酵条件研究

漆酶(E.C.1.10.3.2)是一类含铜的氧化还原酶,其可以催化对苯二酚(也叫氢醌)氧化生成对苯醌,也常被称为对苯二酚氧化酶。漆酶一般存在于真菌和植物中,由于具有较高的稳定性和较好的底物专一性,在印染和环境有机毒性化合物的降解、专用生物传感器的构建、以及造纸领域有机废弃物的生物加工利用等方面具有实际工业应用。考察了白腐菌的培养和产漆酶条件,对白腐菌产漆酶的培养条件和培养基进行了优选。结果显示,以小麦皮为碳源,硫酸铵和尿素为氮源,白腐菌生长产酶的适宜培养温度为28℃,pH值为3.5~6.0,培养基的C/N比以20合适。当培养基中添加一定的木质素沉淀物和葡萄糖时对白腐菌液体发酵产漆酶具有明显的促进作用,菌体发酵液中加入10 g/L的葡萄糖、6 g/L木质素时发酵产漆酶的活力达到最大值。     

超声波降解吲哚废水的实验研究

研究了吲哚模拟废水在超声波作用下的降解, 并对超声波与电化学协同作用下吲哚的降解规律进行了初步探讨. 考察了废水初始浓度、pH值、处理时间、超声功率、外加催化剂以及声电协同作用下电流密度等因素对吲哚降解的影响. 结果表明, 超声处理吲哚的降解规律符合一级反应; 处理时间越长, 降解率越大; 吲哚初始浓度较低时, 降解率较大; 随着 pH值的增大, 降解率先增大后减小, 在 pH=8左右降解率最大; 降解率随超声功率的增大而增大, 在120 W时达最大值, 随后降低; 加入1 .5 mmol/L H2O2, 对吲哚的降解有较大的促进作用. 在超声波存在下, 电解电流在1 0~2. 2 A范围内, 降解率随电流先增大后减少, 电流为1. 9 A时, 降解率达最大值; 声电化协同作用能提高吲哚的降解率.     

白腐菌降解偶氮金属络合染料的研究

针对白腐菌的特性和独特的降解机理,用Corilus versicolor和Panus conchatus两种典型白腐菌降解金属络合染料,结果表明,白腐菌对这类染料有明显的脱色作用,经驯化的白腐菌对染料废液处理72h脱色率可达90％以上,测定了在特定条件下两种白腐菌产锰过氧化物酶（Mnp)和漆酶（Laccase)的活性,讨论了染料的脱色与酶活及菌丝产量高低的相关性,认为白腐菌对该类染料的脱色作用包括物理吸附和酶促降解两个方面。     

ABTS强化HRP/H2O2体系降解吲哚的效能与机制

针对辣根过氧化物酶催化过氧化氢(HRP/H₂O₂)体系存在对污染物降解速度慢的问题,采用2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)作为电子转移体,强化HRP/H₂O₂体系降解吲哚,对不同pH值、ABTS浓度和常见共存水体成分的吲哚降解效能进行研究.通过高效液相色谱-四级杆-飞行时间质谱仪及发光细菌毒性实验,考察吲哚的降解产物及毒性变化.结果表明:在pH值为5.0~11.0的范围内,ABTS可显著强化HRP/H₂O₂体系降解吲哚,且强化效能随ABTS浓度的增加而增加;常见共存水体成分对吲哚的降解均无显著影响;检测到5种吲哚降解产物,其生物毒性相较于吲哚有所下降.     

固定化漆酶催化去除水中2,4-二氯苯酚

采用壳聚糖固定化漆酶催化去除水中的2,4－二氯苯酚,探讨了影响反应的不同因素,并与自由漆酶催化反应的条件进行了对比。结果表明,固定酶催化降解反应的最佳pH值在5.5左右,相对于自由漆酶,其最适pH 值向酸性偏移;降解反应的最适温度范围在30～45 ℃之间,较自由漆酶的反应温度范围增大;载体壳聚糖的吸附作用对降解反应和氯酚的去除率影响不大,氯酚的去除主要是漆酶催化氧化反应的结果;连续重复使用固定化漆酶去除模拟废水中的2,4-DCP,在重复操作6次之后,其降解效率仍能保持60%以上。     

白腐真菌J610-D分泌漆酶处理几种有毒环境污染物的研究

就白腐真菌漆酶对环境中几种常见的有毒污染物的降解效果进行了研究.结果表明:白腐真菌漆酶能够很好地催化氧化芳胺类及其衍牛物,当控制苯胺浓度<800 mg/L、邻苯二胺浓度<150 mg/L时,白腐真菌漆酶对苯胺、邻苯二胺的降解率均可达90%、100%;白腐真菌漆酶对硝基苯类化合物的降解效果不明显,当控制其浓度<150mg/L,对硝基苯化合物的降解率可达80%.     

白腐菌F8的分离及降解木质素能力研究

从松林土壤中分离得到一株白腐菌F8,经Bavendamn氏显色反应证实其能产生漆酶.在液体培养条件下,该菌株能产生木质素过氧化物酶、锰过氧化物酶和漆酶.其降解木质素的最佳条件为100 r/min、33℃、pH5.5、接种量5%,在此条件下,木质素降解率达71.98%.     

白腐菌Pleurotus ostreatus漆酶对蒽醌染料SN4R脱色研究

利用白腐菌Pleurotusostreatus324粗漆酶和纯漆酶进行蒽醌染料SN4R的脱色研究.粗漆酶可使蒽醌染料SN4R脱色,最适脱色pH、温度和漆酶酶活分别为4.0、40℃和30IU/mL,12h染料脱色率为55%.纯漆酶不能使SN4R脱色,但小分子介体物质可介导纯漆酶对染料SN4R的氧化脱色,在最适条件下4h染料脱色率可达80.6%.粗漆酶添加适当浓度的介体物质,可使染料完全脱色.因此,小分子还原介体物质的存在有助于染料废水的降解和脱色.     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

Perspherical Approaches for Electrons in Atoms and Charged Excitons in Quantum Wells

Instead of following Fock’s expansion,we solve the Schrodinger equation for some quantum mechanical manybody systems such as electrons in atoms and charged excitons in quantum wells in a similar way in hyperspherical coordinates by expanding the wave functions into orthonormal complete basis sets of the hyperspherical hannonics(HHs)of hyperangles and generalized Laguerre polynomials(GLPs)of the hyperradius.This leads the equation to     

Advances in broadband wireless communications under high-mobility scenarios

Future mobile communication systems aim at providing very high data transmission rates, even in high-mobility scenarios such as high-speed wheel-track trains, maglev trains, highway vehicles, airplanes, guided missiles or spacecraft. A particularly important commercial application is the strong and increasing worldwide demand for high- speed broadband wireless communications （up to 574.8 km/ h test speeds or 380 km/h commercial speeds） in railways, providing data, voice and video services for applications such as onboard entertainment services to passengers, train control, train dispatch, train sensor status handling and sur- veillance. In such high-mobility scenarios, there are a number of communication challenges, including fast hand- over, location updating, high-speed channel modeling, estimation and equalization, anti-Doppler spreading tech- niques, fast power control, and dedicated network architec- ture. Because signal transmission in very high-speed scenarios will inevitably experience serious deterioration, it is imperative to develop key broadband mobile communi- cation techniques for such high-speed vehicles.     

Wuhan University Journal of Natural Sciences TOTAL CONTENTS

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海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Scale-based spatial data model for GIS

Being the primary media of geographical information and the elementary objects manipulated, almost all of maps adopt the layer-based model to represent geographic information in the existent GIS. However, it is difficult to extend the map represented in layer-based model. Furthermore, in Web-Based GIS, It is slow to transmit the spatial data for map viewing. In this paper, for solving the questions above, we have proposed a new method for representing the spatial data. That is scale-based model. In this model we represent maps in three levels: scale-view, block, and spatial object, and organize the maps in a set of map layers, named Scale-View, which associates some given scales.Lastly, a prototype Web-Based GIS using the proposed spatial data representation is described briefly.     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to