亲环素A抗原表位在三维结构中的初步定位

以抗人亲环素A单克隆抗体D4作捕获抗体，用噬菌体展示库鉴定出亲环素A模拟抗原表位的氨基酸序列为WSLQSFL，在亲环素A的一级结构中没有相同的序列，提示亲环素A抗原表位为构象型的，亲环素A的三维空间结构已测定，利用RasMol等蛋白质三维结构观察软件，可以初步确定亲环素A的抗原表位的时间位置，此抗原表位与环孢素的结合位点有重叠。     

ConA—Sepharose 4B的制备及其对血浆糖蛋白的亲和效率

报道了ＣｏｎＡ－Ｓｅｐｈａｒｏｓｅ４Ｂ的制备方法，经测定Ｓｅｐｈａｒｏｓｅ４Ｂ对ＣｏｎＡ的偶联效率达８５％，该亲和凝胶对血浆糖蛋白有亲和吸附．其亲和效率为１．８ｍｇ（蛋白／ｍｌ凝胶）．     

单克隆抗本亲和层析法纯化人尿激酶

含抗人尿激酶单克隆抗本的腹水经ＰｒｏｔｅｉｎＡ－ＳｅｐｈａｒｏｓｅＣＬ４Ｂ纯化得到ＩｇＧ纯品，分子量为１５０ＫＤ，与ＣＮＢｒ－Ｓｅｐｈａｒｏｓｅ４Ｂ偶联制成抗人尿激酶单克隆抗体－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析介质。     

眼镜蛇膜毒素与单克隆抗体偶联物对鼻咽癌细胞的？…

应用柱层析和凝胶过滤的方法，从中华眼镜蛇的粗毒中分离纯化出一个不含磷脂酶Ａ２（ＰＬＡ２）的膜毒素（Ｍｅｍｂｒａｎｅ ｔｏｘｉｎ，ＭＴ）组分。ＭＴ经电泳鉴定为单一成分。将ＭＴ与鼠抗人鼻咽癌细胞（ＣＮＥ２）的单克隆抗体（ＢＡＣ５）偶联制备成免疫毒素（ＢＡＣ５－ＭＴ），经间接免疫荧光检测，证实免疫毒素仍保留了很好的生物活性。体外抗癌实验证明，在无Ｃａ＾２＋培养其中，游离的ＭＴ对ＣＮＥ２和Ｓ１８０细胞均有     

人亲环素基因的克隆、表达及应用研究

亲环素(CyP)具有多种生物学功能,它是CsA的胞内受体,能和CsA特异结合;它又是人类免疫缺陷病毒(HIV)在细胞内复制的必要因子.研究首先从动物组织中提取、纯化CyP蛋白,并且克隆了CyPA基因,在Ecoli中获得高效表达.进而又克隆、表达了CyPB基因,进行了生物学活性测定,制备了CyP蛋白单克隆抗体和多抗,建立了多项检测方法.研制的CyP自身抗体及CsA血药浓度两种检测药盒已在临床应用,已取得良好的效益.     

含内射极大左理想的左遗传环

证明了Ｒ是含内射极大左理想的遗传环当且仅当Ｒ是如下形式之一的环：（１）Ｒ是半单Ａｒｔｉｎ环；（２）Ｒ环同构于形式三角矩阵环，其中Ａ，Ｂ，Ｃ满足下列条件；（３）Ａ是左遗传，ＢＡ平坦．（４）Ｃ是除环，ＣＢ内射，（５）ａｎｎ（ＢＡ）是内射左Ａ－模，并且Ａ／ａｎｎ（ＢＡ）典范同构于自同态环Ｅｎｄ（ＣＢ）。     

抗氧化修饰低密度脂蛋白单克隆抗体的制备及其初？…

用氧化修饰脂蛋白（ａ）对Ｂａｌｂ／ｃ小鼠免疫，利用淋巴细胞杂交瘤技术制备了鼠抗人氧化修饰低密度脂蛋白（ｏｘｉｄａｔｉｖｅｌｙｍｏｄｉｆｉｅｄｄｅｎｓｉｔｙｌｉｐｏｐｒｏｔｅｉｎ，ｏｘＬＤＬ）单克隆抗体细胞株，以单克隆抗体包被建立了酶联免疫吸附（ＥＬＩＳＡ）双抗夹心法，用此法对１０列冠心病患者，６例其他心脏病患者，１０例正常健康人血浆中ｏｘＬＤＬ的含量进行了测定。结果表明，冠心病患者血浆中ｏｘＬＤＬ     

重组人钙调素的表达及其McAb的制备与精子CaM定位

用基因重组技术构建人钙调素基因Ⅲ（ｈＣａＭⅢｃＤＮＡ）表达载体ｐＢＶ／ｈＣａＭⅢ,并在大肠杆菌ＤＨ５α中经热诱导获得可溶性ＣａＭ蛋白的高效表达．将纯化的重组ｈＣａＭ与异型双功能剂ＳＰＤＰ及鼠血清白蛋白（ＭＳＡ）交联,免疫Ｂａｌｂ／ｃ小鼠,用常规细胞融合技术制备单克隆抗体（ＭｃＡｂ）,得到３株稳定分泌单克隆抗体的杂交瘤细胞．间接ＥＬＩＳＡ和斑点免疫结果证实,三种单克隆抗体均与自制的ｒｈＣａＭ和ＣａＭ标准品起特异反应．用免疫组化法对精子中ＣａＭ定位,发现ＣａＭ主要分布于精子头颈部,不育组ＣａＭ＋精子率（（４５．０±７．５）％）显著低于生育组（（６８．５±１０．５）％）．     

一步法免疫亲和层析纯化重组人肿瘤坏死因子

提出一种简便的免疫亲和层析方法，有效地分离纯化重组人肿瘤坏死因子（ｒＨＴＮＦ），此法不需预先分离纯化单克隆抗体．用交联刘ｄｉｍｅｔｈｙｌｓｕｂｅｒｉｍｉｄａｔｅ使抗ＴＮＦ的单克隆重抗体和ｐｒｏｔｅｉｎＡ—ＳｅｐｈａｒｏｓｅＣＬ－４Ｂ共价结合，成为稳定的免疫基质，制备免疫亲和层析柱，将重组ＴＮＦ粗制品一步纯化为均一组份．     

乙肝病毒基因上一个新的维甲素受体反应元件的发现

在对人乙肝病毒ＡＤＲ－１进行计算机序列检索时，发现在Ｂａｍ ＨＩ和ＨｉｎｃⅡ酶切位点内存在一保守的维甲素受体反应元件序列。用构建好的包含这一片段的乙肝病毒基因亚克隆ｐＢａｍＨＩ－ＨｉｎｃⅡ－ＣＡＴ２与人维甲素Ａ受体ｈＲＡＲα或人维甲素Ｘ受体ｈＲＸＲα共转染人特异性肝癌细胞ＨｅｐＧ２，通过相应配体ＡＴＲＡ或９－ｃｉｓＲＡ的刺激，使细胞内氯霉素乙酰基转移酶活力较不加配体刺激的对照细胞明显增强。为了排除     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.