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| 乙肝病毒基因上一个新的维甲素受体反应元件的发现 | |
| 引用本文： | 陆晔,靳嘉瑞,王耀发,李载平.乙肝病毒基因上一个新的维甲素受体反应元件的发现[J].华东师范大学学报(自然科学版),2000(2):97-103. |
| 作者姓名： | 陆晔 靳嘉瑞 王耀发 李载平 |
| 作者单位： | 1. 上海市疾病控制与预防中心,上海,200336 2. 中科院上海生化所,上海,200031 3. 华东师范大学生物系,上海,200062 |
| 摘 要： | 在对人乙肝病毒ＡＤＲ－１进行计算机序列检索时，发现在Ｂａｍ ＨＩ和ＨｉｎｃⅡ酶切位点内存在一保守的维甲素受体反应元件序列。用构建好的包含这一片段的乙肝病毒基因亚克隆ｐＢａｍＨＩ－ＨｉｎｃⅡ－ＣＡＴ２与人维甲素Ａ受体ｈＲＡＲα或人维甲素Ｘ受体ｈＲＸＲα共转染人特异性肝癌细胞ＨｅｐＧ２，通过相应配体ＡＴＲＡ或９－ｃｉｓＲＡ的刺激，使细胞内氯霉素乙酰基转移酶活力较不加配体刺激的对照细胞明显增强。为了排除 |
| 关 键 词： | 维甲素受体反应元件 基因调控 乙肝病毒基因 |
The Identification of A New Retinoid Responsive Element on HBV Gene |
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| LU Ye,JIN Jia-rui,WANG Yao-fa,LI Zai-ping.The Identification of A New Retinoid Responsive Element on HBV Gene[J].Journal of East China Normal University(Natural Science),2000(2):97-103. | |
| Authors: | LU Ye JIN Jia-rui WANG Yao-fa LI Zai-ping |
| Abstract: | The constructed subclone of HBV (ADR-1) gene p BamHI-HincII-CAT 2 was transfected into the two human hepatoblastoma cell lines Hep G2 and Hela, together with either human retonoid receptor A plasmid o r human retonoid receptor X plasmid and treated with corresponding ligand. The C AT activity of the cells is found to increase significantly after treatment. Fur ther gel retardation assay confirmed that there is a new retonoid acid responsiv e element in the fragment of HBV gene between the restruction enzyme digestion s ite between BamHI and HincII. This element is highly conserved, formed b y two direct repeat sequences 5'-AGGTGT-3' separated by 6 bases. |
| Keywords: | Retinoid acid responsive element CAT as say gel retardation assay gene regulation |
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