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1.
以葛枣猕猴桃越冬芽发出的嫩茎作外植体进行离体培养,用腋芽增殖途径形成再生植株,筛选出了效果较好的诱导培养基:继代增殖培养茎和生根培养基,建立了完整的组培快繁体系。  相似文献   

2.
不同培养基对四季樱草叶再生植株形成的影响   总被引:1,自引:1,他引:0  
以四季樱草幼叶为材料,将其培养在四种不同的培养基上培养,使其形成再生植株,可以发现:培养基不同,形成愈伤组织的诱导率不同,芽分化率、苗的增殖系数也显著不同,结果是I〉Ⅳ〉Ⅲ〉Ⅱ。说明植株再生明显受到激素种类及不同激素组合的影响,证明以MS+ZT3+6BA0.5+NAA0.1+IBA0.1组合的培养基效果为最好。  相似文献   

3.
荔蒲芋的组织培养   总被引:2,自引:0,他引:2  
以荔蒲芋球茎上的萌芽或芽鳞片为外植体,在含有6-BA和NAA的MS培养基上较易诱导出丛生芽和愈伤组织,丛生芽在液体培养基中继代培养可产生大量无根小苗,激素配比对繁殖系数影响较大,以6-BA2.0mg/L和NAA0.6mg/L的配比最适合继代增殖,无根小苗在含有NAA1.0mg/L的1/2MS培养基中诱导15d即呆生根形成再生植株。  相似文献   

4.
以成年态南丰蜜橘茎段为材料,在不同激素配比的MS培养基中进行再生芽的诱导和继代增殖培养,确定了最佳的诱导培养基改良MS+6-BA0.5 mg/L+KT0.5 mg/L+NAA0.1 mg/L,把诱导培养基中的蔗糖浓度由3%提高到6%可以很好地解决再生芽的叶片发黄、脱落问题;再生芽增殖的最佳培养基为改良MS+6-BA0.4 mg/L+ZT0.1 mg/L+NAA0.05 mg/L,增殖系数达5.40.SDS-PAGE电泳结果表明33.4 kD相对分子质量的蛋白谱带出现于再生芽正常分化生长时期,可能是维持再生芽生长发育的特异蛋白组分.  相似文献   

5.
【目的】以铁线莲‘朱卡’(Clematis ‘Julka’)的带芽茎段为起始材料,通过组织培养方法建立再生体系。【方法】利用腋芽诱导—不定芽增殖—生根和愈伤组织诱导—增殖—分化—生根两种途径,建立该品种的组织培养再生体系,形成铁线莲‘朱卡’完整植株。【结果】铁线莲‘朱卡’组织培养最适宜灭菌条件为质量分数10%次氯酸钠溶液灭菌12min;带芽茎段诱导腋芽最适培养基为MS+NAA 0.1 mg/L+6-BA 1.0 mg/L,不定芽增殖最适培养基为MS+IBA 0.20 mg/L+6-BA 1.0 mg/L+GA3 0.2 mg/L;带芽茎段诱导愈伤组织最适培养基为MS+NAA 0.05 mg/L+6-BA 2.0 mg/L,愈伤组织增殖最适培养基为MS+NAA 0.20 mg/L +6-BA 2.0 mg/L,愈伤组织分化最适培养基为MS+NAA 0.03 mg/L+6-BA 3.0 mg/L;最佳生根培养基为1/2 MS+IBA 0.3 mg/L。【结论】首次用两种器官发生方法建立了稳定高效的铁线莲‘朱卡’再生体系。直接形成不定芽时增殖倍数可达5.52。诱导愈伤组织途径的器官发生中,愈伤组织分化率可达76.7%,分化不定芽平均数超过3。两种途径诱导生根率都在90%以上。  相似文献   

6.
三倍体无籽西瓜的组织培养   总被引:2,自引:0,他引:2  
将三倍体无籽西瓜带子叶幼芽以及成株顶芽和腋芽培养在附加BA2mg·L-1的MS培养基中,芽体均可以扩增为芽丛.并首次报道了花芽在培养基中展开后子房愈伤组织也可以再生不定芽.不同途径得到的幼芽进一步扩增或转移到MS附加IAA0.2mg·L-1的培养基后,均可以分化不定根,从而形成完整植株.  相似文献   

7.
为建立文心兰(Oncidium)丛生芽工厂化继代增殖途径技术体系,对培养基、激素种类、激素浓度配比和继代增殖次数等影响丛生芽增殖增殖分化的关键因子进行比较研究。结果表明:以文心兰花梗为外植体,添加2.5~3.0 mg/L TDZ与0.2~0.4 mg/L NAA组合和1g/L活性炭和亚硫酸钠后诱导丛生芽分化丛生芽或原球茎效果好。TDZ与NAA浓度比值为8.93或8.75时,丛生芽诱导分化率最高,为90.0%,增殖系数为6.0~7.4;丛生芽分化途径最佳继代增殖培养基为:1/2MS+10 g/L琼脂+30 g/L蔗糖+1g/LAC+1g亚硫酸钠+2.8 mg/LTDZ+0.32 mg/LNAA;经5-6代继代增殖培养后,丛生芽变异率仅为0.1%。  相似文献   

8.
大花萱草--金娃娃的初代培养和无性系的建立   总被引:4,自引:0,他引:4  
外植体及取材部位对大花萱草愈伤组织的诱导和丛生芽的形成都有显著的影响,实验证明花托是理想的外植体取材部位,适当的培养基可使花托的诱导率达100%;培养基中6-BA和NAA不同的浓度比及不同的取材部位,使增殖系数及丛生芽的质量有明显差异,实验中丛生芽的增殖系数最高可达16。  相似文献   

9.
在大石花菜切段悬浮培养的研究基础上,为进一步改善石花菜切段育苗技术,本文研究了温度和辐照度对切段苗种培育的影响,包括切段的再生、再生芽生长、匍匐体的形成与生长。培育初期可选择较低辐照度,适宜的条件是辐照度40μEm ̄(-2)s ̄(-1)和20.0℃的温度。  相似文献   

10.
以茉莉的下胚轴为外植体,研究了茉莉直接不定芽器官发生途径的再生体系.结果表明:以MS为基本培养基,附加0.5 mg/L BAP和0.01 mg/LNAA的诱导效果最佳,其不定芽再生率高达92%,外植体平均不定芽数目为4.2.适宜不定芽增殖的最佳培养体系为MS+1.0 mg/L BAP+0.1 mg/L NAA.1/2MS+0.5 mg/L IBA+0.5 mg/LIAA+100mg/L适于再生幼茎的生根,生根率达91%,生根后经移栽成活.  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.  相似文献   

13.
王慧 《科技信息》2008,(10):240-240
Wuthering Heights, Emily Bronte's only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.  相似文献   

14.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

15.
Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.  相似文献   

16.
以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.  相似文献   

17.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

18.
Quality traits in wheat (Triticum aestirum L.) were studied by quantitative trait locus (QTL) analysis in a recombinant inbred line (RIL) population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross (ChSh). Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.  相似文献   

19.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

20.
赵英华 《科技信息》2008,(12):219-220
0.Preface China has long been boasting of its colorful and immense culture which comprises of Taoism,Confucianism and Buddhism.The interwoven influence among the religions on Chinese way of thinking,on the nation's behavior in their social life throughout the long history is beyond list.  相似文献   

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