3,5—二溴水杨醛缩4—氨基安替比林与铜的荧光猝灭反应？…

研究了新荧光试剂３,５－二溴水杨醛缩４－氨基安替比林（简称：３,５－ＤＢＳＡＡＰ）与铜的荧光猝灭反应体系的最适宜的条件。３,５－ＤＢＳＡＡＰ发射强的荧光,λｅｘ／ｅｎ＝３９３ｎｍ／５１０ｎｍ。在ｐＨ９．０的ＮＨ３－ＮＨ４Ｃｌ缓冲介质中,Ｃｕ（Ⅱ）与３,５－ＤＢＳＡＡＰ在室温反应使其荧光猝灭。据此建立了铜的分析方法,线性范围为１．６×１０＾－５ｍｏｌ／Ｌ￣１．２×１０＾－７ｍｏｌ／Ｌ。用于测定人发中     

新试剂6-(2-羟基-4-二乙基氨苯偶氮)-2,3-二氢-1.4-酞嗪二酮的电化学发光研究

应用自制的ＥＣＬ－１型电致化学发光仪，对新试剂６－（２－羟基－４－二乙基氨苯偶氮）－２．３－二氢－１，４－酞嗪二酮（ＨＤＥＡ）在Ｎａ２ＣＯ３－ＮａＨＣＯ３缓冲介质中的电化学发光行为作了研究．结果发现，当介质ｐＨ值为 １０．５４时，在一定的实验条件下，施加 １．７Ｖ （ＶＳ．ＳＣＥ）三角波脉冲电压，体系的发光强度与ＨＤＥＡ浓度在５．０ × １０－９～１．０×１０－６ｍｏｌ／Ｌ范围内呈线性关系，反应的检测限达１．０×１０－９ｍｏｌ／Ｌ．     

长毛对虾酸性磷酸酶的纯化与性质

分别从健康和患病长毛对虾（Ｐｅｎａｅｕｓｐｅｎｉｃｉｌａｔｕｓ）肌肉提取一种酸性磷酸酶（ＡＣ－Ｐａｅ，ＥＣ．３．１．３．２．），进一步用硫酸铵分级分离，ＳｅｐｈａｄｅｘＧ－２００柱纯化，经聚丙烯酰胺凝胶电泳，并于ＢｅｃｋｍａｎＤＵ－８Ｂ紫外分光光度计扫描为单一区带酶液。该酶紫外吸峰在２８０ｎｍ处，荧光激发峰在２８４ｎｍ处，发射峰在３４７ｎｍ处。酶的分子量为７３０００道尔顿，等电点为４．７酶水解对硝基苯磷酸二钠（ＰＵＰＰ）最适ｐＨ为４．５，最适温度４０℃。健康虾与病虾酶的活化能分别为４１．０３ｋＪ／ｍｏｌ·Ｌ和４５．７８ｋＪ／ｍｏｌ·Ｌ，米多常数ｋｍ值分别为０．８０×１０－４ｍｏｌ／Ｌ和１．４３×１０－４ｍｏｌ／Ｌ。重金属离子Ｃｕ２＋，Ｈｇ２＋，有机溶剂甲醇，乙醇，乙二醇等对ＡＣＰａｓｅ有明显的抑制作用。     

卤代烃对唑化合物荧光猝灭机理

测试了１×１０－５ｍｏｌ／Ｌ５，５－二苯基－２，２－联唑（ＰＯＯＰ）、反－１，２－双［２－（５－苯基唑基）］乙烯（ＰＯＥＯＰ）、４，４－双［２－（５－苯基唑基）］－１，１－联苯（ＰＯＢＯＰ）在含不同浓度卤代烃的１，４－二氧六环溶液中的荧光量子产率，讨论了荧光猝灭机理，计算出ＣＨ３Ｉ、ＰｈＩ、ＰｈＢｒ对ＰＯＥＯＰ、ＰＯＯＰ、ＰＯＢＯＰ的荧光猝灭速率常数．研究发现，卤代烃分子越小，卤原子核电荷数越高，其猝灭荧光能力越强     

Meso-[四(对-三甲铵基)苯基]卟啉在核酸分子表面长距组装的荧光特性及分析应用

在低离子强度下，ｍｅｓｏ［四（对三甲铵基）苯基］卟啉（ＴＡＰＰ）能在核酸分子表面进行长距组装并诱导核酸超螺旋结构的形成．长距组装使得ＴＡＰＰ的Ｓｏｒｅｔ带发生减色效应，并使Ｓｏｒｅｔ带处激发产生的荧光被猝灭．体系离子强度（Ｉ）增大将降低ＴＡＰＰ长距组装的能力，导致荧光猝灭程度降低．在Ｉ＝０００４时，荧光猝灭程度与核酸的浓度成线性关系，可用于１５×１０－６～９０×１０－６ｍｏｌ／Ｌ的小牛胸腺ＤＮＡ，１５×１０－６～１０５×１０－６ｍｏｌ／Ｌ鱼精子ＤＮＡ和酵母ＲＮＡ的测定．     

5—Br—PADN双峰双波长法测定铁（III）

研究５－Ｂｒ－ＰＡＤＮ与铁（Ⅲ）的显色反应，在ｐＨ９．０￣９．４范围内，当有１０％ＳＤＳ存在时，铁（Ⅲ）与５－Ｂｒ－ＰＡＤＮ形成１：４的稳定配合物。λｍａｘ为５３４ｎｍ，ε为５．１３×１０＾４Ｌ·ｍｏｌ＾－１·ｃｍ＾－１，铁（Ⅲ）浓度在０￣１２．５×１０＾－４ｇ／Ｌ范围内符合Ｂｅｅｒ定律，用双峰双波长法测定茶叶中痕量铁（Ⅲ），结果满意。     

5－Br－PADN双峰双波长法测定铁（Ⅲ）

研究５－Ｂｒ－ＰＡＤＮ与铁（Ⅲ）的显色反应，在ｐＨ９．０～９．４范围内，当有１０％ＳＤＳ存在时，铁（Ⅲ）与５－Ｂｒ－ＰＡＤＮ形成１：４的稳定配合物。λ＿（ｍａｘ）为５３４ｎｍ，ε为５．１３×１０￣４Ｌ·ｍｏｌ￣（－１）·ｃｍ￣（－１），铁（Ⅲ）浓度在０～１２．５×１０￣（－４）ｇ／Ｌ范围内符合Ｂｅｅｒ定律，用双峰双波长法测定茶叶中痕量铁（Ⅲ），结果满意。     

镝(Ⅲ)-1,10-双(1′-苯基-3′-甲基-5′-氧代吡唑-4′-基)癸二酮-[1,10]-溴化十六烷基三甲胺体系的荧光性质及其应用研究

研究了镝（Ⅲ）－１,１０－双（１′－苯基－３′－甲基－５′－氧代吡唑－４′－基）癸二酮－［１,１０］（ＢＰＭＰＤＤ）－溴化十六烷基三甲胺（ＣＴＭＡＢ）体系的荧光性质,探讨了体系的组成比及影响体系荧光性质的多种因素,拟定了应用该体系测定Ｄｙ（Ⅲ）的新方法,在２０×１０－６～４０×１０－９ｍｏｌ·Ｌ－１范围内Ｄｙ（Ⅲ）浓度与荧光强度呈线性关系,检测限为３３×１０－１０ｍｏｌ·Ｌ－１．将该法应用于合成样品中微量Ｄｙ（Ⅲ）的测定,结果满意     

2－羟基－3－羧基－5－磺酸基苯重氮氨基偶氮苯与阳离子表面活性剂显色反应的研究

研究了溴化十六烷基三甲胺（ＣＴＭＡＢ）、溴化十六烷基吡啶（ＣＰＢ）、氯化十六烷基吡啶（ＣＰＣ）、氯化十四烷基二甲基苄基胺（Ｚｅｐｈ）等四种阳离子表面活性剂（ＣＳＦ）与２－羟基－３－羧基－５－磺酸基苯重氮氨基偶氨苯（ＨＣＳＤＡＡ）的显色反应。＂在ＴｒｉｔｏｎＮ－１０１存在下和ｐＨ１１．３～１２．１的缓冲介质中，各种ＣＳＦ均与ＨＣＳＤＡＡ形成摩尔比为１：１的紫色离子缔合物，其最大吸收波长是５８５ｎｍ，对比度为１５４ｎｍ，在３２～４０℃时表观摩尔吸光系数都为４．０×１０３Ｌ·ｍｏｌ－１·ｃｍ－１。ＣＳＦ在２×１０－６～１．０×１０－４ｍｏｌ／Ｌ范围内服从比尔定律。在ＥＤＴＡ的掩蔽作用下，大量常见离子对反应不干扰。可望用于阳离子型和阴离子型表面活性剂的分析。     

借IAP-4S和高效液相色谱分离和测定铱和铑（英文）

借离子对反相高效液相色谱分离了铱（Ⅳ）和铑（ＩＩＩ） 的２（２咪唑偶氮）苯酚４磺酸（ＩＡＰ４Ｓ）螯合物,并于５６０ ｎｍ 波长处检测之．研究了Ｉｒ（ Ⅳ）和Ｒｈ（ Ⅲ）ＩＡＰ４Ｓ螯合物分离的最宜条件．于Ｎｕｃｌｅｏｓｉｌ Ｃ８ 柱上,用含７．５×１０－３ ｍｏｌ／ＬＫＨ２ＰＯ４Ｎａ２ＨＰＯ４缓冲剂（ｐＨ７．５） 和５．５ ×１０－３ ｍｏｌ／Ｌ溴化四丁基铵的甲醇水（４０：６０,ｖ／ｖ） 作流动相,流速为０．８ ｍＬ／ｍｉｎ． 检测限为１．３ ｎｇ 铱和１．５ ｎｇ 铑．     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.