PMP柱前衍生HPLC法测定松乳菇多糖的单糖组成

建立了一种1-苯基-3-甲基-5-吡唑酮（PMP）柱前衍生高效液相色谱（high performance liquid chromatography,HPLC）法测定松乳菇多糖中单糖组成。通过单因素实验优化PMP衍生单糖的条件为反应时间40 min,反应温度70°C和PMP浓度0.8 mol/L。以Agilent Zorbax SB-C18色谱柱为分析柱,磷酸缓冲液（0.05 mol/L,pH=6.9）-乙腈为流动相,检测波长为250nm,进样量10μL,柱温20°C检测单糖组成。在此色谱条件下,各单糖线性关系良好（r2≥0.999 6）,精密度高（RSD≤2.23%）,重复性强（RSD≤2.11%）,加标回收率为97.16%～101.68%。该方法适用于松乳菇多糖中单糖组成的测定。     

柱前衍生高效液相色谱法测定红牛维生素功能饮料中牛磺酸含量

用邻苯二甲醛(OPA)柱前衍生高效液相色谱法,运用C18色谱柱为固定相,甲醇 0.05mol/L NaAc溶液(45 55)为流动相,检测波长330nm,测定红牛维生素功能饮料的牛磺酸.结果衍生化反应迅速,分离效果好,在10～50μg/ml的浓度范围内,牛磺酸色谱峰面积与浓度之间的线性相关系数为0.9999,加标回收率为97.48%～101.0%,RSD为2.5%.     

二氟化硼-二吡咯甲烷(BODIPY)类荧光试剂柱前衍生高效液相色谱分离荧光检测磷酸化氨基酸

建立了采用新型荧光试剂1,3,5,7-四甲基-8-苯基-(4'-O-(N-琥珀酰亚胺乙酸酯))-二氟化硼-二吡咯甲烷(TMPAB-OSu)为衍生化试剂的柱前衍生高效液相色谱分离荧光检测分析方法测定三种磷酸化氨基酸.在最佳的实验条件下,方法测定磷酸化丝氨酸(P-Ser)、磷酸化酪氨酸(P-Tyr)、磷酸化苏氨酸(P-Thr)的线性范围为0.05～2.5×10-6mol/L;检出限为2×10-9mol/L.     

高效液相色谱-荧光检测法(HPLC-FLD)测定水体中孔雀石绿

实验旨在建立水样中孔雀石绿测定的液相色谱-荧光检测方法.水样中孔雀石绿采用二氯甲烷为溶剂超声波辅助提取,提取液离心分离后经MCX柱净化,并用5%氨水甲醇溶液洗脱,以Develosil C18色谱柱为分析柱,0.05 mol/L的乙酸铵(pH 4.5)-乙腈(20:80,V/V)为流动相,采用荧光检测器分析,外标法定量.结果表明:孔雀石绿在0.05μg~1.0μg/mL范围内线性良好,相关系数为0.9993,检出限为0.5μg/L.空白水样在加标孔雀石绿含量分别为1.0μg、2.5μg、5.0μg时,孔雀石绿的平均加标回收率为72.0%~86.4%,相对标准偏差为5.4%~7.5%.该方法准确度高、精密度良好,适用于水产养殖用水中孔雀石绿的测定.     

HPLC法测定长春西汀原料的有关物质研究

建立高效液相色谱法测定长春西汀有关物质的方法.色谱柱C_(18)柱(4.6×250 mm,5μm),流动相为乙腈-乙酸铵(0.2 mol/L)(55∶45),流速为1.0 m L/min,进样量为20μL,检测波长为280 nm.结果表明,依据本法检测,长春西汀的线性关系良好,专属性强;精密度和稳定性良好.该方法准确、灵敏、专属性强,可用于长春西汀的检测.     

HPLC-ELSD法测定盐酸氨基葡萄糖胶囊中盐酸氨基葡萄糖的含量

采用高效液相色谱-蒸发光散射检测法测定了盐酸氨基葡萄糖胶囊的含量.使用氨基色谱柱(250 mm×4.6 mm,5μm),流动相组成为乙腈:水=(7:3),流速为1.0 m L/min,在盐酸氨基葡萄糖检测含量浓度范围为0.2~1.2 mg/m L时与峰面积呈线性关系,R2为0.9999,平均加样回收率为99.86%,RSD为1.37%.该法简单,准确,无需衍生反应,精密度和重现性较好,是盐酸氨基葡萄糖胶囊含量测定的有效方法.     

双波长HPLC-荧光法同时测定褪黑素和维生素B6

建立了双波长高效液相色谱-荧光检测器同时检测保健品中褪黑素和维生素B6含量的方法.考察了不同超声时间、清洗次数对保健品中褪黑素和维生素B6溶出量的影响,并对色谱条件进行了优化.采用Inertsil-100A C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-水(65∶35,体积比)为流动相,流速1.0 mL/min,柱温25℃,进样量10μL.采用双波长法,0～4 min,λ_(ex)=332 nm,λ_(em)=397 nm;4 min后,λ_(ex)=286 nm,λ_(em)=352 nm.在优化的样品预处理条件、色谱条件下,维生素B6和褪黑素分别在0.1～100μg/L和1.0～1 000μg/L内线性关系良好(r≥0.998),最低检出限分别为3×10~(-3)μg/L和3×10~(-2)μg/L;定量限分别为1×10~(-2)μg/L和1×10~(-1)μg/L,平均回收率分别为99.07%和97.97%(n=3),相对标准偏差为0.04%～0.98%.本方法检测快速,灵敏,重现性较好,可用于保健品中褪黑素和维生素B6的同时测定以及市售保健品的质量控制.     

牛奶中硫氰酸盐高效液相色谱测定方法

建立了高效液相色谱法分析牛乳中硫氰酸盐的方法.以戴安IonPac AS19(4 mm×250mm)阴离子色谱柱进行分离.二极管阵列检测器测定,流动相为0.05 mol/L Na2HP04溶液(pH值约为9.3),流速为0.8 mL/min,进样量10μL,检测波长218 nm.结果表明,牛奶中硫氰酸盐线性范围为5～100μg/mL,加标回收率为67.1%～101.8%,相对标准偏差(n=3)小于4.4%,最低检出限为0.5μg/mL.     

高效液相色谱分析L-瓜氨酸的研究

文中建立了L-瓜氨酸的HPLC检测方法,实验应用2,4二硝基氟苯(DNFB)柱前衍生化法将L瓜氨酸衍生后在HPLC上进行定量分析,探讨和优化了L-瓜氨酸的HPLC分析色谱条件。研究结果表明,L 瓜氨酸样品检测线性范围为2～10μg ,相关因数r=0.9990,精密度为1.7%。该方法灵敏,重复性好,结果准确,分析迅速,能有效的指导L-瓜氨酸的发酵生产。     

HPLC法测定枸橼酸坦度螺酮片含量和有关物质

采用高效液相色谱法,用Luna C_(18)柱(250×4.6mm,10μm),以乙腈-0.01mol·L~(-1)磷酸二氢钾溶液(35:65,pH3.0)为流动相,流速:1ml/min,柱温:25℃,进样量20μL,检测波长:239nm.在该色谱条件下枸橼酸坦度螺酮和杂质峰达到良好分离(R1.5),枸橼酸坦度螺酮在1.0~200.0μg·ml~(-1)范围内线性关系良好(r=0.9991);重复性及精密度良好(RSD2%),3种浓度的平均回收率分别为100.17%、99.03%及99.75%(n=3).方法快速、简便、准确,适用于枸橼酸坦度螺酮片的含量测定和有关物质检查.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.