紫球藻培养动力学模型的构建

以紫球藻FJ-12为实验对象，在敞开式柱型鼓泡光生物反应器中研究尿素质量浓度对其生长和生物活性物质合成的影响.结果表明，白光条件下，200 mg·L^-1尿素质量浓度培养的紫球藻生物量达到最大(3.10 g·L^-1),胞外多糖质量浓度最高(542.55 mg·L^-1).此外，200 mg·L^-1尿素条件下，紫球藻合成藻红蛋白的能力较强.多不饱和脂肪酸含量与初始尿素质量浓度呈正相关，亚油酸含量则呈负相关.动力学模型结果显示，尿素被用于紫球藻生长、藻红蛋白和胞外多糖的部分合成及细胞其他活动消耗.综上，最适合紫球藻生长和活性物质合成的尿素质量浓度为200 mg·L^-1,该条件下的动力学模型为规模化养殖紫球藻和合成相关活性物质提供了指导.     

NaCl胁迫对紫球藻生长和多糖含量的影响

在此就NaCl胁迫对紫球藻生长和多糖含量的影响进行探讨．在盐胁迫条件下，紫球藻的生长受到抑制，叶绿素和藻红素的含量均下降；但结合多糖的浓度随着培养时间而显著增加．盐胁迫作用还迅速抑制紫球藻的光合作用效率并增加暗呼吸速率。     

5种不同形式氮源对紫球藻生长影响的研究

在含有相等氮量的不同表式氮源的培养中培养紫球藻（Porphyridium purpareum),观察5种不同表式氮源对紫球藻生长的影响,结果表明,硝酸盐促进紫球藻的生长,铵盐则不利于其生长,脲介于这二者之间。     

不同光质对紫球藻生长及藻胆素含量的影响

利用绿光、蓝光、红光、白光(对照)4种光质培养紫球藻,通过测定藻密度和藻液的光吸收以比较不同光质对紫球藻生长的影响,同时测定藻胆素含量,可溶性总蛋白的含量．实验结果表明:绿光培养条件下紫球藻的生物产量、藻胆素、可溶性总蛋白的含量最高,蓝光次之,而在红光培养条件的紫球藻生长最缓慢．实验数据为紫球藻的高密度放大培养提供依据．     

紫球藻对抗生素的生物学效应

研究了基因工程中常用抗生素标记对紫球藻的生物学效应,结果表明紫球藻对青霉素、链霉素、庆大霉素以及卡那霉素较不敏感,剂量在100u/mL以下对其生长无显著影响,但能抑制或杀灭伴生的杂菌,因此这些抗生素可用于藻种纯化过程中抑菌;紫球藻对红霉素、氧氟沙星和土霉素非常敏感,低质量浓度就能杀死藻细胞,可用作基因工程或遗传育种的抗药性选择标记;对四环素和氯霉素较为敏感,明显抑制细胞生长,并呈浓度梯度效应.     

基于机械搅拌式光照发酵罐的紫球藻高产藻红蛋白培养条件优化

本研究以普通机械搅拌式光照发酵罐为培养装置,采用单因素试验法对紫球藻生产藻红蛋白的条件（搅拌速率、光照强度、氮浓度以及NaCl浓度）进行优化。结果表明,发酵培养紫球藻高产藻红蛋白的最佳搅拌速率、光照强度、氮浓度以及NaCl浓度分别为150 r/min、2 000 Lx、8 mmol/L、20‰。本试验优化了搅拌式光照发酵罐培养紫球藻生产藻红蛋白的条件,为紫球藻藻红蛋白的工业化生产提供基础数据及思路。     

高盐环境下紫球藻对其他逆境的适应性研究

紫球藻在盐逆境的胁迫下，所产生的感应反映和适应机理有可能使其适应其他的逆境。在此以生长在1.5mol／L NaCl环境下并达到对数期的紫球藻为试验材料,观察测定光抑制或低温对光合作用等主要生理生化参数的影响,发现：经过盐处理的紫球藻在光抑制或低温逆境下,光合效力下降更快更低。     

海带中3-吲哚乙酸对海洋微藻生长代谢的影响

利用反向高效液相色谱、UV光谱扫描和质谱方法对海带(Laminaria japonicaAresch.)提取物中的3-吲哚乙酸进行定性和定量分析,结果表明海带提取物中含有3-吲哚乙酸.在此基础上,利用反向高效液相色谱方法对3-吲哚乙酸进行制备,并研究其对小球藻(Chlorellasp.)、盐藻(Dunaliellasalina)、角毛藻(Chaetoceros muelleri)、紫球藻(Porphyridiumcruentum)和球等边金藻(Isocrydid galbana)5种海洋微藻生长代谢的影响.实验表明海带3-吲哚乙酸浓度为0~100μmol/L时,小球藻、盐藻和紫球藻细胞生长均被促进;海带3-吲哚乙酸浓度对小球藻和紫球藻胞内蛋白质含量影响显著,对小球藻叶绿素含量影响极显著.研究为海带生长素类物质活性及海洋中大型藻与微藻生长关系的研究奠定了基础.     

紫球藻多糖的分离纯化及抗菌活性

从紫球藻中提取的水溶性粗多糖,用Sevage法脱蛋白后,经DEAE-纤维素52柱层析可得到纯品．利用圆形纸片法对紫球藻多糖提取物进行了抑菌实验．结果表明,紫球藻多糖提取物对稻瘟病病原菌和赤皮病病原菌有较强的抑制作用,其抗细菌活性与抗真菌活性没有明显差别,对稻瘟病病原菌的最小抑菌浓度为6．25mg／mL．     

搅拌式光生物反应器培养紫球藻的条件优化

研究在光生物反应器中搅拌速度、通气量、光照强度和装液量对紫球藻生长的影响.实验结果表明:在摇床培养过程中,紫球藻的生物量和胞外多糖随着三角瓶装液量的减少而增加.通过正交试验对搅拌式光生物反应器的三个培养条件通气量、搅拌转速和光照强度进行优化,获得搅拌式光生物反应器培养紫球藻的最佳条件为通气量120L/h、搅拌转速200r/min和光照强度5000lx,在该条件下紫球藻的生物量为2.548g/L、胞外多糖557.2mg/L.正交试验分析表明,三个培养条件中通气量对藻生物量和胞外多糖含量的影响最明显.通过采用DPS软件对正交实验结果进行二次多项式模型分析和拟合,以生物量和胞外多糖为指标建立回归模型,得到相应的优化条件和预测值.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with