| 海带中3-吲哚乙酸对海洋微藻生长代谢的影响 |
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| 引用本文： | 李铁松,王长海.海带中3-吲哚乙酸对海洋微藻生长代谢的影响[J].辽宁师范大学学报(自然科学版),2010,33(1). |
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| 作者姓名： | 李铁松 王长海 |
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| 作者单位： | 1. 辽宁师范大学,生命科学学院,辽宁,大连,116029
2. 大连理工大学,环境与生命科学学院,辽宁,大连116024;烟台大学,海洋学院,山东,烟台,264005 |
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| 基金项目： | 国家高技术研究发展计划(863计划)项目(2001AA620704);;辽宁省教育厅科学技术研究项目(2008A350);;辽宁师范大学博士启动基金项目 |
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| 摘 要： | 利用反向高效液相色谱、UV光谱扫描和质谱方法对海带(Laminaria japonicaAresch.)提取物中的3-吲哚乙酸进行定性和定量分析,结果表明海带提取物中含有3-吲哚乙酸.在此基础上,利用反向高效液相色谱方法对3-吲哚乙酸进行制备,并研究其对小球藻(Chlorellasp.)、盐藻(Dunaliellasalina)、角毛藻(Chaetoceros muelleri)、紫球藻(Porphyridiumcruentum)和球等边金藻(Isocrydid galbana)5种海洋微藻生长代谢的影响.实验表明海带3-吲哚乙酸浓度为0~100μmol/L时,小球藻、盐藻和紫球藻细胞生长均被促进;海带3-吲哚乙酸浓度对小球藻和紫球藻胞内蛋白质含量影响显著,对小球藻叶绿素含量影响极显著.研究为海带生长素类物质活性及海洋中大型藻与微藻生长关系的研究奠定了基础.
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| 关 键 词： | 海带 3-吲哚乙酸 分离 海洋微藻 生长 |
Effects of indole-3-acetic acid from the kelp Laminaria japonica Aresch. on the growth and metabolism of marine microalgae |
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| LI Tie-song,WANG Chang-hai.Effects of indole-3-acetic acid from the kelp Laminaria japonica Aresch. on the growth and metabolism of marine microalgae[J].Journal of Liaoning Normal University(Natural Science Edition),2010,33(1). |
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| Authors: | LI Tie-song WANG Chang-hai |
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| Institution: | 1.School of Life Science/a>;Liaoning Normal University/a>;Dalian 116029/a>;China/a>;2.School of Environmental and Biological Science and Technology/a>;Dalian University of Technology/a>;Dalian 116024/a>;3.School of Marine/a>;Yantai University/a>;Yantai 264005/a>;China |
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| Abstract: | In the view of the facts that algal extracts have been used in agriculture as a source of plant growth stimulating agents and Indole-3-acetic acid(IAA)has been shown to be present in the extracts,a study was planned to establish the identification of IAA in the kelp(Laminaria japonica Aresch.)extract and study the regulative function for lower plants by the effects on the growth and the biochemical metabolism of the four marine microalgae Chlorella sp.,Dunaliella salina,Chaetoceros muelleri,Porphyridium cru... |
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| Keywords: | brown alga indole-3-acetic acid isolation marine microalgae growth |
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