扩展青霉(Penicillium expansum)PF898基因组DNA提取方法的比较

利用CTAB法、SDS改进法和SDS快速法3种DNA提取代表性方法,对扩展青霉(Penicillium expansum)PF898基因组DNA的提取进行比较研究.CTAB法提取的DNA样品纯度最高,SDS裂解法制备的DNA得率最高;CTAB法和SDS改进法所得DNA都可直接应用于限制性酶切分析;3种方法所提取的DNA均能满足扩展青霉PF898脂肪酶基因和18S rRNA的PCR扩增.总体而言,SDS法简便、快速、得率高,是一种理想的扩展青霉基因组DNA提取方法.     

蒲公英药材总DNA的提取

采用改良的CTAB法、SDS法、高盐低pH法提取蒲公英药材基因组总DNA,通过紫外吸收、琼脂糖凝胶电泳检测其纯度与浓度.结果表明,改良的CTAB法所得DNA纯度最高,电泳条带最清晰、完整性好,可作为RAPD,ISSR等分子生物学研究.     

棉花枯萎菌gDNA不同提纯方法的比例研究

以棉花枯萎菌菌株为试验材料,在CTAB法,SDS－CTAB法等基础上国以改进,对4种提纯棉花枯萎菌DNA的方法进行了比较研究,结果表明,SDS－CTAB法是适合于棉花枯萎菌DNA提取的方法,该方法提取的DNA纯度高,产率高,无明显降解现象,能很好地被限制性内切酶酶切,并用作PCR模板,同时对DNA提取过程中的细节问题进行了探讨与分析。     

葡萄总DNA提取方法的比较研究

以葡萄幼叶为材料，分别采用溴化十六烷基三甲基铵(CTAB)法、高盐低pH法、简易法、高盐沉淀法和改良十二烷基硫酸钠(SDS)法5种方法提取总DNA，通过A260／A280值、琼脂糖凝胶电泳和RAPD对5种方法所得DNA溶液进行定量、定性分析和综合比较。结果显示高盐低pH法、高盐沉淀法综合效果较好，改良SDS法所得DNA纯度最佳。因此，在实际工作中还应根据实验目的选取最适合的方法。     

建兰DNA的快速提取

用SDS高盐提取介质简便快速提取建兰(Cymbidiumensifolium)嫩叶DNA.提得的DNA分子大小约为48kb,A260/A280=1.77～1.86,DNA得率为每g鲜叶410～1145μg.提取的DNA无需经RNase处理,可直接用于限制性内切酶酶切和随机扩增多态DNA反应.     

从动物血液中提取基因组DNA方法的比较研究

从血液中提取基因组DNA的传统方法是先得到淋巴细胞,再用蛋白酶K、SDS消化,然后用酚、氯仿抽提,乙醇沉淀．CTAB法提取基因组DNA多应用于植物和微生物,从全血中提取基因组DNA报道甚少．采用改良的CTAB法从全血中提取基因组DNA,并以传统方法和TAKARA Blood Genome DNA Extraction Kit作对照．结果表明：CTAB法可以得到大量的、较完整的基因组DNA,并且纯度达到1．8～2．0,可以满足PCR扩增和限制酶切等实验的要求．     

一种提取植物基因组DNA的方法——改良尿素法

介绍了一种从玉米和水稻叶片中提取高质量基因组DNA的改良尿素法．与其他常用方法如CTAB法、SDS法等相比,这种改良方法具有产率高、快速省时、可常温操作、成本耗用低等优点．结果证明,采用这种改良方法获得的基因组DNA纯度高、浓度大,适合用于限制性内切酶的酶切反应、基于PCR基础上的遗传多样性分析、基于传统的Southern杂交的遗传图谱分析以及其他下游分子生物学研究,是一种适于植物基因组DNA制备方法,值得推广的．并针对基因组制备过程中经常出现的难题,分析了产生的原因,提出了具体解决办法．     

濒危植物翅果油树DNA的提取方法及纯度检测

为从翅果油树(Elaeagnus mollis Diels)叶中提取高质量的基因组DNA,用PVP和TNE洗液预处理样品并筛选出合适的清洗次数,比较了SDS法、CTAB法和改进CTAB法3种DNA提取方法.结果表明:TNE和PVP预处理三次并且采用改进的CTAB法更适合于翅果油树基因组DNA提取.该方法提取的DNA经紫外分光光度法检测,其A260/A280为1.82,优于CTAB法(1.59)、SDS法(1.00).琼脂糖凝胶电泳和PCR扩增结果也得出同样的结论.     

枣树基因组DNA提取方法及RAPD体系的建立

以8个枣树品种的嫩叶为材料,比较了不同的DNA提取方法,结果表明：采用改良的CTAB法提取的DNA纯度和产率,虽然不比改良SDS的高,但适宜RAPD分析．在20μL反应体积中,RAPD分析的优化反应体系为：引物0．2pmol·L^-1、Mg^2＋2．0mmol·L^-1、dNTP200μmol·L^-1、TaqDNA聚合酶1．2U．改良的CTAB法是枣树RAPD分析基因组提取DNA的最好方法．     

木麻黄小枝基因组DNA的快速提取研究

提取介质中加入2.5% β-巯基乙醇, 能有效去除次生物质对木麻黄基因组DNA提取的影响.用新建方法所提DNA产率比用前人方法提的高出0.8倍左右.鉴定结果表明:鲜小枝、干小枝的DNA样品皆为48kb左右,适于限制性酶切和RAPD反应; 同时发现同一株树的鲜小枝、干小枝,基因组DNA RAPD扩增可得到相同的产物.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.