重组人载脂蛋白AⅠ米兰突变体在酵母中的分泌表达

将重组质粒pPIC9K-apoAⅠ-milano用BglⅡ酶切后转化Pichia pastoris GS115菌株,筛选得到G418高抗性转化子,经甲醇诱导和摇瓶发酵,用SDS-PAGE与Western blot检测上清液,发现有明显的重组人载脂蛋白AⅠ米兰突变体(rAIM)表达,但其分子量比正常蛋白小.对酵母工程菌的摇瓶发酵条件进行优化后,rAIM表达水平得到提高, 约为70 mg/L发酵液,其中正常大小的rAIM所占的比例约为80%.采用冷丙酮沉淀法初步纯化了酵母工程菌发酵液中的rAIM,并验证其有磷脂结合能力.     

人Cu，Zn-SOD在重组毕赤酵母中表达的发酵工艺研究

以表达人Cu,Zn-SOD的重组毕赤酵母为出发菌株,通过摇瓶实验研究发酵初始pH值、诱导剂浓度、诱导温度和培养基组成等因素对目的蛋白表达水平的影响.实验结果表明,培养基组分对目的蛋白表达水平的影响最大.与YPG培养基相比,菌株在FM22培养基(含0.15%组氨酸及PTM4)中目的蛋白表达量提高5.5倍.在优化摇瓶发酵条件下(诱导剂浓度1%,诱导温度27℃,FM22培养基(含0.15%组氨酸及PTM4),初始pH值为6.0),发酵液上清酶活水平为895 U/mL,较初始提高8.5倍;以摇瓶实验结果指导5 L罐发酵,得到13 539 U/mL酶活,为摇瓶试验的15倍.     

6-BA和柠檬酸处理对香石竹切花的影响

试验采用30 mg.L-16-BA和250 mg.L-1CA的配方分别处理香石竹切花,实验结果表明:6-BA能明显提高香石竹切花的质量,延长瓶插寿命。     

响应面法优化L-异亮氨酸产生菌的摇瓶发酵条件

对黄色短杆菌突变株BM2610生产L-异亮氨酸的摇瓶发酵条件进行了研究.单因素优化了菌株BM2610的发酵培养基与培养条件,确定了发酵培养基的最佳碳氮源、最适起始pH、装液量及接种量等,进一步通过响应面分析方法优化了发酵培养基的组成配比.结果在优化条件下,BM2610摇瓶发酵平均积累L-异亮氨酸16.11 g·L~(-1),比未优化前提高了126.3%.     

红曲色素液体深层酵工艺研究

本报道了以玉米为主要原料,采用红曲霉菌,通过液体深层发酵生产红曲色素的最佳培养基组成及其相关工艺条件的选择。摇瓶结果表明,按最佳培养基配方,以8-10%的接种量,在32℃经过72-96hrs的摇瓶培养,发酵液红色素色价基本稳定在120u/ml。最高达150-160u/ml,湿菌体收率为培养液的12%,湿菌体色价为800-1000u/g。     

摇瓶培养条件下桑木层孔菌不同培养期多糖含量动态积累规律研究

采用滤纸片法测定菌丝体生物量以及苯酚-硫酸法测定不同培养期的桑木层孔菌多糖含量和发酵液中残糖含量,研究了摇瓶培养条件下不同培养期的桑木层孔菌多糖含量的动态积累规律.结果表明,桑木层孔菌在摇瓶中培养12d时,获得的菌丝体最多,干重达13.580g/L;培养8d时得到最多的胞外、胞内多糖,含量分别达4.585g/L和32.465mg/g;培养到12d时发酵液中残余糖量最少,为4.744g/L.该实验为进一步研究和开发利用桑木层孔菌多糖奠定了实验基础和科学依据.     

利用稻草液体发酵产纤维素酶及酶粉提取的研究

利用摇瓶确定的优化培养基配方和产酶条件,在30 L罐中研究了里氏木霉HC-415菌利用稻草液体发酵产纤维素酶发酵液pH值、纤维素酶活性等随时间变化的动态规律,研究了发酵液纤维素酶的提取及得率等.所得未脱盐冻干纤维素酶粉CMC酶活性平均为355.0 IU/g,FPA平均为44.3 IU/g.相对发酵液得率平均为16.00...     

有盐条件下微生物对石油的降解效果研究

通过摇瓶试验,研究含盐量为7 000 mg·L-1、石油含量分别为10 000 mg·L-1,30 000 mg·L-1,50 000 mg·L-1条件下,空白对照组(不添加微生物)、细菌组、真菌组分别在5,12,19,26,33 d时对石油的降解效果,并对比分析了降解效果.结果表明,经过33 d后,空白对照组中石油含量未变化,细菌组石油含量为10 000 mg·L-1一组的石油降解率最高,达到了65.69%,真菌组石油含量为30 000 mg·L-1一组石油降解率最高,达到了45.84%.可见,在较高的含盐量条件下,细菌和真菌对石油的降解也仍然有显著效果.     

茶薪菇液体发酵条件的研究

采用液体摇瓶培养法,对茶薪菇液体发酵的培养基pH、最适温度、摇瓶装量、摇瓶转速及不同液体培养基配方进行了探讨,结果表明,茶薪菇液体发酵的培养基初始pH为6.0-6.5,最适温度为25℃,摇瓶装量为50-70ml/250ml三角瓶,摇瓶转速为120-150rpm,最适培养基配方为以麸皮为主要成分的培养基。     

兰花炭疽病拮抗细菌Bacillus megaterium 1-12菌株的产芽孢条件优化

为了提高兰花炭疽病拮抗菌巨大芽孢杆菌(Bacillus megaterium)1-12菌株的芽孢形成率及芽孢数量,在摇瓶发酵基础上对芽孢形成的主要影响因素进行了考察.通过单因素实验和正交实验对1-12菌株产芽孢条件进行了分析,确定了摇瓶发酵最佳条件:培养基组成(质量分数)为玉米粉1%,大豆蛋白胨1%,CaCl2·2H2O·"0.1%,MnSO4·H2O 0.05%;培养液初始pH 6.0;培养条件为种龄20 h,装瓶量30 mL/250 mL,接种量8%,温度30 ℃,摇瓶转速200 r/min.在此条件下,B. megaterium 1-12菌株发酵液中的芽孢数量为1.92×109个mL-1,芽孢形成率达到了95%.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

The Analects and the Moment in Peking——The influence of a Confucian education system on the personality formation,the way of thinking and the fate of the main characters

0.Preface China has long been boasting of its colorful and immense culture which comprises of Taoism,Confucianism and Buddhism.The interwoven influence among the religions on Chinese way of thinking,on the nation＇s behavior in their social life throughout the long history is beyond list.