HPLC法测定银黄口服液中黄芩苷的含量

采用效液相色谱法,色谱柱为Kromasil 100-5 ODS2 C18(5μm,250 mm×4.6 mm),柱温为室温,流动为相甲醇-水-0.1 mol/L磷酸(45.0∶55.0∶0.2),流速为1 mL/min,检测波长为280 nm,建立了银黄口服液中黄岑苷的含量测定方法.在上述条件下,黄芩苷在0.002 72μg·mL-1~0.054 40 mg·mL-1(r=0.999 1)范围内具有良好的线性关系,平均回收率为100.07%,RSD=1.13%.该法简单、准确且快速,可作为银黄口服液中黄芩苷的含量测定方法.     

反相高效液相色谱法测定太太美容口服液中阿魏酸的含量

建立了太太美容口服液中阿魏酸含量的HPLC测定方法.色谱柱为Shim-PackVP-ODS柱(150mm×4·6mm,5μm);流动相为甲醇水冰醋酸(32∶66∶2);流速1mL/min;323nm处进行检测.阿魏酸的线性范围为0·252~5·04μg/mL,r=0·9993,平均回收率为99·9%(n=5),相对标准偏差为1·2%.实验证明该方法简便、快速、准确,适合于太太美容口服液中阿魏酸含量的测定.     

高尿酸血症鸡模型饲料中维生素A醋酸酯的含量测定

目的建立测定高尿酸血症鸡模型饲料中维生素A含量的高效液相色谱法。方法采用十八烷基硅烷键合硅胶柱Kromasil C18(250 mm×4.6 mm,5μm);以甲醇-水溶液(97∶3)为流动相,流速为1.0 m L·min~(-1);检测波长为325 nm;柱温为25℃。结果维生素A在40-800μg·m L~(-1)范围内线性关系良好,r=0.999 9,回收率为101.5%,RSD=2.43%。结论该方法操作简便快速,准确稳定,重现性好,可用作鸡饲料中维生素A含量测定方法。     

凝固型老鹰茶风味发酵乳发酵工艺研究

利用单因素试验和Box-Behnken试验对老鹰茶风味发酵乳工艺参数进行优化,其最佳工艺参数为:鲜奶添加量88%,老鹰茶添加量5.08%,低聚果糖和低聚异麦芽糖比例为1∶1,低聚糖添加量为7.73%;嗜热链球菌∶保加利亚乳杆菌∶植物乳酸杆菌=1∶1∶1,接种量6.25%,发酵温度40℃,发酵时间7h.在此条件下,老鹰茶风味发酵乳具有良好的感官品质,主要理化指标符合GB19302的相关规定,乳酸菌数为1.7×107 CFU·mL~(-1),茶多酚含量为4.1mg·mL~(-1).     

雏痢净超微粉中苍术素含量的测定——以HPLC法为例

目的:建立以HPLC法测定雏痢净超微粉中的苍术素含量的方法。方法:采用Thermo C18色谱柱(250mm×4.6mm,5μm),甲醇-水(79∶21)为流动相,流速1.0mL·min-1,检测波长为340nm。结果:苍术素在0.102μg·mL-1~2.04μg·mL-1范围内与峰面积呈良好的线性关系(R2=1.000 0),精密度RSD=0.098 4%(n=6),重复性RSD=1.168%(n=6),供试品溶液在24 h内稳定性良好,平均加样回收率为99.20%(RSD=0.959%)。结论:该方法操作简便、准确、重现性好,可用于复方微粒的质量控制。     

乳酸菌发酵山药果蔬饮料配方与工艺优化

以铁棍山药、葡萄、番茄、胡萝卜、苹果、蜂蜜为原料,优化出由50%山药浆、10%葡萄汁、20%番茄汁、10%胡萝卜汁和10%苹果汁组成的原浆,用植物乳杆菌CZ、植物乳杆菌CA、植物乳杆菌CB按照1∶1∶1比例制成的发酵剂,按照原浆78%、蜂蜜3%、蔗糖13%和发酵剂6%的比例配制混合,32℃发酵8h,获得功能型乳酸菌发酵山药果蔬饮料.其蛋白质、脂肪、还原性糖、钠和维生素C的质量浓度及pH值依次为0.004kg·L~(-1)、0.0121kg·L~(-1)、0.080kg·L~(-1)、0.000 182kg·L~(-1)、0.000 102kg·L~(-1)和4.0±0.2.     

HPLC法测定黄精丸中阿魏酸的含量

目的:建立了HPLC测定黄精丸中阿魏酸含量的方法.方法:用AglientTC-C18(4.6mm×250mm,5μm)色谱柱,0~15min,乙腈-0.085%磷酸溶液(20∶80)为流动相,流速为1mL·min-1,检测波长为316nm,柱温35℃,进样体积10μL,应用安捷伦1200系列液相色谱仪进行测定.结果:阿魏酸与其他成分分离良好,在0.0129μg~0.1161μg范围内呈良好的线性关系(r=0.999998),平均回收率(n=6)为99.96%.结论:该方法简便、准确,重复性好,可用于测定黄精丸中阿魏酸含量.     

正交试验设计优选通脉软膏的辅料组成及其渗透性能与皮肤安全性研究

制备通脉软膏并评价其体外经皮渗透特性及皮肤安全性.以物理性状为指标,参考姜黄素含量筛选通脉软膏辅料比例,选择君药姜黄的主要有效成分姜黄素进行经皮评价,选用Thermo Hypersil GOLD C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈-4%冰乙酸溶液(48∶52)为流动相,检测波长为425 nm,采用改良的Franz扩散池分别开展通脉软膏及药粉传统外敷用法的体外经皮吸收试验,并对软膏的致敏性进行评估.结果显示,通脉软膏的最佳配方为凡士林∶羊毛脂∶液体石蜡=40∶10∶15,姜黄素的含量为0.2389 mg·g~(-1),24 h累计透皮量为0.0411 mg·g~(-1),累计透皮率为17.27%,所制得的通脉软膏无致敏性.制备的通脉软膏性状较好,其体外透皮性能明显优于传统外敷形式,且皮肤安全性较好.     

血样中3-烟酰胺基-1,2,4-苯并三嗪-1,4-二氧化物的高效液相色谱分析

建立高效液相色谱法分析血样中的3-烟酰胺基-1,2,4-苯并三嗪-1,4-二氧化物(SR-N)。Agilent Eclipse C18柱(4.6 mm×150 mm,5μm),流动相为甲醇-水-冰醋酸=20∶80∶0.5,流速1.0 mL·min-1,柱温25℃,检测波长277 nm。在选定的液相色谱条件下,SR-N血浆标准曲线在0.75~120μg·mL-1范围内呈良好线性关系(r=0.999 4)。检测限和定量限分别为0.25、0.75μg·mL-1。     

HPLC法测定阿司匹林经皮给药贴剂中阿司匹林的含量

建立阿司匹林贴剂含量的测定方法。乙腈为阿司匹林贴剂的萃取溶剂,用Waters公司Symmetry Shield^TM RP₁₈柱(3.9mm×150mm, 5μm),流动相：乙腈-0.01mol·L^-1的磷酸氢二钠（磷酸调pH值至2.50）;体积比为25∶75;检测波长234nm;流速10mL·min^-1。阿司匹林在3～96μg·mL^-1范围内呈现良好的线性关系,r=0.9993。阿司匹林回收率为100.3%,RSD为0.42%。本方法简单、灵敏、准确,可作为该制剂内在质量的控制方法。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Jane Eyre——A Feminist In The 19th Century

In the 19th century the society was controlled by men, and women were just appendants of them, they had not any rights and freedom. But Jane was an exception, she showed some characteristics of early feminist. Jane showed her characteristics of feminism in three aspects: rebellion, equality, and independence. These characteristics were helpful to her success, and feminism is the only way out for women of that time.     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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