雏痢净超微粉中苍术素含量的测定——以HPLC法为例

目的:建立以HPLC法测定雏痢净超微粉中的苍术素含量的方法。方法:采用Thermo C18色谱柱(250mm×4.6mm,5μm),甲醇-水(79∶21)为流动相,流速1.0mL·min-1,检测波长为340nm。结果:苍术素在0.102μg·mL-1~2.04μg·mL-1范围内与峰面积呈良好的线性关系(R2=1.000 0),精密度RSD=0.098 4%(n=6),重复性RSD=1.168%(n=6),供试品溶液在24 h内稳定性良好,平均加样回收率为99.20%(RSD=0.959%)。结论:该方法操作简便、准确、重现性好,可用于复方微粒的质量控制。

The Determination of the Mass Fraction of Atractylodin in Superfine Powder of Chinese Medicine Chulijing---Taking the Method of HPLC(High Performance Liquid Chromatography) as an Example

Objective： The method of HPLC with UV detection for the determination of atractylodin in the Chulijing superfine powder was established. Method： The chromatographic conditions included that： Thermo C18（250 mm×4.6 mm,5μm）was used as chromatographic column;the methanol- water（79∶21）as mobile phase;the flow rate was 1.0 m L/min,the detection wave length was 340 nm. Result： The calibration curves for atractylodin were linear in the range of 0.102 ~2.04ug·ml-1（R2=1.000 0）,RSD of instrument precision was 0.098 4%（n=6）,RSD was 1.168%（n=6）,samples＇ solution was stable within 24 h,and the average recovery was 99.20%（RSD =0.959%）.Conclusion： The method, which is easy and simple to handle with good repeatability,can be used for the quality control of the compound Chulijing superfine powder.