血清浓度对SMMC—7721细胞DNA合成的影响

以人肝癌ＳＭＭＣ－７７２１细胞为材料研究了不同血清浓度对ＤＮＡ合成和细胞生长状态的影响，结果表明几乎在所有处理中短时无血清培养都能刺激ＳＭＭＣ－７７２１细胞的ＤＮＡ合成，且＾３Ｈ－ＴｄＲ掺入量与培养液中ＦＣＳ浓度成明显负相关，＾３Ｈ－ＴｄＲ掺入量比值最高可达３９．３２倍．１８ｈ内，随培养时间的增加无血清培养对细胞ＤＮＡ合成的刺激作用逐渐下降，＾３Ｈ－ＴｄＲ掺入量比值从３９．３２倍下降为３．５３倍，     

锌缺乏对小鼠周围血淋巴细胞增殖反应的抑制效应

本实验测定锌缺乏时小鼠周围血淋巴细胞（ＰＢＬ）对有丝分裂ＣｏｎＡ刺激反应的改变。当用不同锌含量的合成饲料饲养Ａ／Ｊ小鼠２８天后，缺锌组小鼠体重和总食物消耗明显减少，体重仅为对照组的６８％。当用５μｇＣｏｎＡ／ｍｌ刺激ＰＢＬ时，中度和重度缺锌组细胞３￣Ｈ－ＴｄＲ掺入显著减少，分别为对照组的５２％和３１％，而限制组与对照组相比无明显改变。结果说明：缺锌时周围血淋巴细胞免疫功能受到严重损害，而不是由于食物摄入不足所致。     

MTT比色分析法在贴壁生长细胞药物敏感性测定中的应用

用ＭＴＴ法测定了几种小檗胺类化合物对三种贴壁生长细胞生长增殖的影响。结果显示：ＭＴＴ分析法比传统的活细胞计数法，单细胞克隆尖及＾３Ｈ－ＴｄＲ掺入法测定样品，省时，省试剂，精确度高，重复性好及没有同位素污染等优点，是肿瘤药物研究中较理想的检测方法。     

金属离子与伴刀豆球蛋白A刺激细胞作用的关系研究

采用＾３Ｈ－ＴＤＲ掺入法，研究了过渡金属离子Ｃｄ＾２＋、Ｎｉ＾２＋、Ｃｕ＾２＋和Ｚｎ＾２＋取代Ｃｏｎ－Ａ中Ｍｎ＾２＋之后，对人淋巴细胞刺激分裂作用影响。研究结果表明，不同的金属离子取代后的Ｃｏｎ－Ａ刺激活性都明显提高。     

咖啡因对胎鼠及乳鼠睾丸生殖细胞数量的影响

为了了解咖啡因对胚胎及新生时期男性生殖系统的影响，采用低（０３ｍｍｏｌ／Ｌ）、中（０６ｍｍｏｌ／Ｌ）、高（１２ｍｍｏｌ／Ｌ）浓度咖啡因体外培养ＳＤ孕１８ｄ胎鼠、０ｄ及４ｄ乳鼠睾丸组织块，用ＨＥ染色及放射自显影方法检测咖啡因对睾丸内生殖细胞数量及其摄取３Ｈ－ＴｄＲ的影响。结果：１８ｄ胎鼠睾丸培养组织内生殖细胞数量及３Ｈ－ＴｄＲ的摄取受咖啡因影响较少；中等浓度的咖啡因在培养３周后，生殖细胞的数量、３Ｈ－ＴｄＲ的摄取才降低；而高浓度的咖啡因在培养２周后，检测指标已有下降，３周更明显。生殖细胞数量的减少往往伴随３Ｈ－ＴｄＲ摄取的降低，由此推测高浓度咖啡因长时间培养后使生殖细胞数量减少可能与抑制生殖细胞摄取３Ｈ－ＴｄＲ，从而干扰ＤＮＡ的合成有关。     

海脉冲营养素（HMC）研究：HMC对小鼠免疫功能的影响

研究海脉冲营养素“ＨＭＣ”对小鼠免疫功能的影响。结果表明：小鼠口服ＨＭＣ３００ｍｇ／ｋｋｇ．ｄ，连续７－１０ｄ，可增加小鼠免疫器官的重量；提高小鼠腹腔巨噬细胞吞噬率和吞噬指数；促进ＰＨＡ刺激的淋巴细胞转化反应和提高ＡＮＡＥ＾＋淋巴细胞百分率；     

无血清培养细胞HICSF的自分泌生长因子的检测

为了探讨无血清培养细胞ＨＩＣＳＦ的自分泌调控机制，采用ＲＴ－ＰＣＲ技术检测生长因子ｂＦＧＦ，ＴＧＦβ１和细胞因子ＩＬ－６，ＩＬ－８，Ｇ－ＣＳＦ以及细胞因子受体ＩＬ－６Ｒ和ＩＬ－８Ｒ在ＨＩＣＳＦ细胞及其同基因来源的血清培养细胞ＨＩＣ中的表达状况，同时用＾３Ｈ－ＴｄＲ掺入法观察重组ＩＬ－６和ＴＧＦβ１对这２种细胞生长的影响，以观察癌细胞在有血清和无血清培养条件下部分生长因子和细胞因子的表达及反应性差异     

2-(H酸偶氮)-4,5-二硝基酚光度法测定铝的研究和应用

研究了新试剂２－（Ｈ－酸偶氮）－４,５－二硝基酚（ＨＡＤＮＰ）与铝的显色反应,在ＰＨ５．３的六次甲基四胺－盐酸缓冲介质中,ＨＡＤＮＰ与Ａｌ＾３＋生成３比１的紫色络合物,λｍａｘ＝５７０ｎｍ,ε＝３．２９＊１０＾４Ｌ．ｍｏｌ＾－１．ｃｍ＾－１,Ａｌ质量浓度在０－４８０μｇ．Ｌ＾－１内符合比尔定律,方法用于水样测定,结果满意。     

串珠镰孢菌诱导的棉花细胞的反应性氧迸发

串珠镰孢菌的孢子和细胞壁水解物可诱导棉花悬浮培养细胞产生反应性氧迸发。来自抗性品种豫棉８号的培养细胞表现为二次反应性摒迸发。培养细胞的生长状态，激发子浓度、反应介质中Ｃａ＾２＋和Ｋ＾＋等金属离子含量、Ｈ＾＋－ＡＴＰ酶的抑制剂（钒酸盐），ＮＡＤＰＨ氧化酶抑制剂和人工电子受体Ｆｅ（ＣＮ）６＾３－均影响细胞的反应性氧迸发水平。     

混合金属离子溶液中形成复合氢氧化物的研究

采用电位滴定法对Ⅲ－Ⅱ价混合金属离子（Ａｌ＾３＋、Ｆｅ＾３＋、Ｎｉ＾２＋、Ｚｎ＾２＋、Ｃｕ＾３＋）水溶液加碱形成复合氢氧化物进行了研究,结果发现,所的Ｍ＾３＋￣Ｍ＾２＋混合体系均能形成复合氢氧化物,并且复合氢氧化物沉淀时的ＰＨ值低于单一金属离子析出Ｍ（ＯＨ）２深沉时的ＰＨ值,这种ＰＨ值差别越大,越易形成复合氢氧化物,即形成复合的顺序是：Ｎｉ＾２〉Ｚｎ＾２＋〉Ｃｕ＾２＋值,这种ＰＨ值差别越大,越易形     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.