高效液相色谱法测定不同类别食品中的叶黄素

为了研究不同类别食品中不同形态叶黄素的分析方法,针对不同样品基质和叶黄素形态,分别选用超声波辅助提取法、酶解—超声波提取法和碱皂化—液液萃取法,建立并优化了测定叶黄素的高效液相色谱法。采用C_(18)柱(4.6 mm×250 mm,5μm)进行检测,以甲醇—水为流动相进行等度洗脱,流速为1.0 mL/min,柱温为35℃,检测波长为446 nm,叶黄素在0.125～10.0 mg/L范围内呈良好线性,相关系数为0.999 91。不同类别食品的加标回收率为90.0%～102.2%,方法检出限为0.10 mg/kg。采用该方法对各类食品样品中叶黄素的含量进行了测定,实验结果表明该方法适用范围广、针对性强、准确度高和重现性好,适用于各类食品中叶黄素的检测。     

复方肝舒丸的质量标准研究

目的:建立复方肝舒丸的质量标准.方法:采用薄层色谱法对丸剂中的丹参、大黄、当归、白术进行定性鉴别.采用高效液相色谱(HPLC)法测定丸剂所含丹参酮ⅡA的量,色谱柱为Welchrom-C18柱(5μm,250 mm×4.6mm),流动相为甲醇-水(体积比80∶20),流速1.0 m L·min-1,柱温30℃,检测波长为270 nm.采用HPLC法测定大黄素、大黄酚的含量,色谱柱为Welchrom-C18柱(5μm,250 mm×4.6 mm),流动相为甲醇-0.1%磷酸溶液(体积比90∶10),柱温25℃,流速1.0 m L·min-1,检测波长为254 nm.结果:薄层斑点清晰,阴性对照无干扰,专属性强.丹参酮ⅡA在0.02~0.2μg范围内线性关系良好(r=0.9999),平均加样回收率为99.26%,RSD为0.66%(n=9).大黄酚在0.2~2.0μg范围内线性关系良好(r=0.9999),平均加样回收率为99.21%、RSD为0.72%(n=9).大黄素在0.2~2.0μg范围内线性关系良好(r=0.9999),平均加样回收率为99.21%,RSD分别0.61%(n=9).结论:本方法操作简单、灵敏度高、结果准确、重现性好、专属性强,可作为复方肝舒丸的质量控制标准.     

RP-HPLC定量检测蛹虫草培养基中活性成分

建立一种反向高效液相色谱法定量检测蛹虫草培养基中活性成分腺苷含量和虫草素含量的方法.色谱柱为Eclipse XDB-C18柱(4.6 mm×150 mm,5μm);流动相:0.02 mol/L KH2PO4∶甲醇(体积比85∶15)溶液;流速:1.0 mL/min;检测波长:260 nm.腺苷的线性测量范围为0.5~100μg/mL(R=0.999 9),平均回收率为105.3%,标准品RSD=0.76%(n=5),样品RSD=1.87%(n=5);虫草素的线性测量范围为0.5~100μg/mL(R=0.999 9),平均回收率为104.0%,标准品RSD=0.95%,样品RSD=1.23%.本测定方法线性范围宽、分离度好、具有良好精密度,可定量、准确分析不同蛹虫草培养基中活性成分腺苷和虫草素含量.     

HPLC法测定脑血栓片中丹酚酸B含量

采用高效液相色谱法测定脑血栓片中的丹酚酸B含量,利用C18色谱柱(4.6 mm×150 mm,5μm),流动相为乙睛-0.2%磷酸(21:79),流速为1.0 mL/min,检测波长λ=286 nm,柱温40℃,结果表明丹酚酸B检测浓度在0.031 76～1.985μg(Y=967.602 7X-2.419 1)范围内与峰面积值线性关系良好(r=0.999 9),平均回收率为100.39%(RSD=0.62%).本方法灵敏、简便、重现性好,结果准确,可用于脑血栓片的质量检测.     

布洛芬胶囊含量的高效液相色谱法测定

建立了测定布洛芬含量的高效液相色谱法.选用Agilent Eclipse XDB-C8柱(4.6 mm ×250 mm,5μm),Lab Alliance C8保护柱(4.6 mm×10mm),流动相为甲醇水=7525,流速1.0 mL/min,检测波长220nm,进量样20μL,柱温室温.在10.0～400μg/mL浓度范围内,线性方程为A=52.50 C+50.79,相关系数0.999 9,回收率96.0%～100.7%,RSD 0.65%～1.32%.该方法简便、准确、快速,可用于布洛芬的质量控制.     

高效液相色谱法测定肉苁蓉中麦角甾苷的含量

目的:建立了肉苁蓉中麦角甾苷的含量测定方法.方法:采用Kromasil C18.(4.6mm×250mm,5μm)色谱柱,流速1ml/min.流动相:甲醇-水(含磷酸0.04%)为35:65;检测波长为331nm;柱温35℃.结果:麦角甾苷在0.068-2.72μg范内与峰面积呈良好线性关系(回归方程为A=192.57C-2.896,r=0.9999),平均回收率为98.7%,RSD为1.41%(n=5).结论:本方法简便、重现性良好、结果准确可靠.     

HPLC测定妇炎净软胶囊中阿魏酸的含量

建立以高效液相色谱法测定妇炎净软胶囊中阿魏酸含量的方法。色谱柱为Diamonsil C18(5μm,250×4.6mm,5μm);流动相为甲醇-1%醋酸(35:65);流速1ml.min-1;检测波长:320nm。阿魏酸的线性范围是0.05055～0.4044μg(r=0.9999),平均回收率为100.4%,RSD=1.45%(n=5)。本方法简便、快捷、结果准确,可用于妇炎净软胶囊的质量控制。     

HPLC法测定罗库溴铵中溴丙烯的含量

为建立以高效液相色谱法(HPLC)测定罗库溴铵中溴丙烯的含量的方法,实验色谱条件为,色谱柱为C18(Kromasil 250 mm×4.6 mm,5μm),流动相为0.05 mol.L-1四甲基氢氧化铵溶液(用磷酸调节pH值至5.0)—乙腈(4∶6),流速为1.0 mL.min-1,检测波长为210 nm,进样量为5μL.结果表明,以溴丙烯浓度(C)对峰面积(A)进行回归分析,回归方程为,A=34337.41C-2682.68,r=0.9999.在检测浓度为,0.528μg/mL～105.6μg/mL范围内呈良好线性关系,平均回收率为97.6%～100.2%,RSD=0.9%(n=9),精密度试验RSD=0.9%.实验证实,采用HPLC法测定罗库溴铵中溴丙烯的含量,方法灵敏度高,专属性强,结果准确可靠.     

HPLC法测定蒙药巴特日七昧丸中没食子酸的含量

目的:建立测定蒙药巴特日七味丸中没食子酸含量的方法.方法:采用高效液相色谱法.应用Sphefisorb C18(250mm×4.6mm,5μm)色谱柱;以甲醇-0.1%磷酸水溶液(15:85)为流动相;流速为0.8ml·min-1;检测波长为273nm;柱温为25℃.结果:没食子酸在0.102μg～0.306μg范围内,与峰面积具有良好的线性关系,r=0.999 9;平均回收率为98.89%、RSD为1.41%(n=6).结论:该方法简便、快捷、准确、重复性好,可用于巴 特日七味丸中没食子酸的含量测定.     

HPLC法测定维生素口服液中烟酰胺质量浓度

采用高效液相色谱法测定维生素口服液中烟酰胺的质量浓度.色谱柱为ODS-C18柱(HypersilODS2,4.6mm×150mm,5μm),流动相为1-癸烷磺酸钠溶液(1.22→850)∶乙腈∶磷酸=850∶150∶1,流速为1.0mL/min;检测波长为280nm.烟酰胺在10～100μg/mL范围内呈良好的线性关系(r=1 0000),平均回收率为98.33%,RSD=0.66%(n=9).结果表明,本方法简便,准确,快速,灵敏度高、重现性好.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Jane Eyre——A Feminist In The 19th Century

In the 19th century the society was controlled by men, and women were just appendants of them, they had not any rights and freedom. But Jane was an exception, she showed some characteristics of early feminist. Jane showed her characteristics of feminism in three aspects: rebellion, equality, and independence. These characteristics were helpful to her success, and feminism is the only way out for women of that time.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-