日本对虾血清和肌肉提取液凝集活力初步研究

用12种天然或经酶修饰的动物及人红细胞测定日本对虾的血清和肌肉提取液的凝集活力，并且进行糖抑制、热稳定性、pH及Ca^2 影响试验。结果显示：血清和肌肉提取液均能凝集12种红细胞，但两者对每种红细胞的凝集效价各不相同。血清对鹤鹑红细胞的凝集作用可被蔗糖抑制，最小抑制浓度为200mmol/L，其凝集活性依赖于Ca^2 ，在pH4.0-7.0范围内较稳定，经90℃保温10min保持最高活性；肌肉提取液的凝集活性不被7种糖抑制,在pH6.0-7.0，温度为4－80℃范围内均具有最高活性，其凝集活性不依赖于Ca^2 。由以上结果推测：血清与肌肉提取液均含有凝集素，且种类可能不同。     

泥蚶体液凝集素性能的初步研究

在泥蚶(Tegillarca granosa Linnaeus)体液中发现有凝集素存在.利用14种天然或经酶处理的动物和人红细胞来测定泥蚶体液凝集素的凝集活力,并且进行糖(200 mmol·L-1)抑制、热稳定性、pH及Ca2 影响试验.结果显示:泥蚶体液凝集素对14种红细胞均有凝集作用.其中对兔红细胞的凝集作用可被乳糖和半乳糖所抑制,凝集活性依赖于Ca2 ,在pH7.0较稳定,温度为40～60℃范围内均具较高活性,经90℃保温10 min后活性完全消失.     

福建7种双壳类凝集素凝集作用的研究

以福建沿海7种常见的双壳类为材料,研究其血清及肌肉提取液对10种脊椎动物血细胞、8种单细胞藻类、5种微生物细胞和5种真菌孢子的凝集性能．实验结果表明,7种双壳类的血清或肌肉提取液中均含有凝集素,能凝集一种或多种类型的红细胞、单细胞藻类及微生物细胞．且不同种类对细胞的凝集效应不同,产生凝集作用所需的最低蛋白质浓度相差很大．在供试的细胞中,兔红细胞、紫球藻和啤酒酵母对双壳类凝集素最敏感．     

12种食用菌凝集素的筛选

12种食用菌提取液经磷酸缓冲液抽提，硫酸铵沉淀而获得．用不同类型的细胞检测其凝集活性，结果表明：有10种食用菌提取液能凝集2种或2种以上的供试细胞，表现出凝集活性．但不同菌种凝集素的凝集能力、凝集活性及热稳定性有很大的差异，抑制凝集活性的糖的种类也有所不同，这可能与它们的组分及化学结构有关．     

粘虫幼虫血清,血浆及血细胞裂解液凝集活性的比较研究

利用血凝试验及半抗原抑制试验分别测定了粘虫Ｍｙｔｈｉｍｎａ ｓｅｐａｒａｔａ（Ｗａｌｋｅｒ）幼虫血清、血浆和血细胞裂解液凝集红细胞的能力及专性抑制凝集的糖种类。结果显示：血清和血浆均能凝集鸡、羊及人Ｂ型血红细胞，而且对同种红细胞的凝集活力完全相同；然而血细胞裂解液只凝集羊红细胞，对鸡和人Ｂ型血红细胞不凝集。糖抑制试验表明：血清或血浆对人Ｂ型血红细胞的凝集被乳糖及岩藻糖抑制，而对羊红细胞的凝集则被昆     

4种腹足纲动物粗蛋白的凝集作用

采用来自5种脊椎动物的8种血细胞、7种微生物单细胞、5种藻类细胞，对4种海产腹足纲动物凝集素进行筛选，均检测到凝集活性。绵羊红细胞、啤酒酵母、紫球藻对凝集反应最为敏感。4种动物凝集素普遍不耐高温，60℃或65℃下15min即全部失活，凝集反应的适宜pH为6．5-8．0，其凝集性能可能分别被不同的糖所抑制，但均可被0．02mol／LEDTA抑制。     

杂色鲍血清凝集素的初步研究

研究结果表明,杂色鲍血清中自然存在凝集素,该凝集素能够识别和凝集多种不同的细菌和人及动物的红细胞,其中对人体O型和B型血细胞的凝集效价显著高于A型和AB型血细胞(P<0.05);Ca2+能促进其凝集活性,而Mg2+则抑制其凝集活性;凝集作用不同程度地受反应介质的pH、离子强度及反应时间等因素的影响;该凝集素不被透析,在95℃、pH2.0～10.0时仍保持凝集活性,抗RNase、DNase、SDS和乙醚,对蛋白酶K、糖氧化剂NaIO4敏感;凝集素活性能被D-树胶醛糖、L-鼠李糖特异性抑制,D-果糖、D-木糖对其也有显著的抑制作用.     

福建11种软体动物凝集素的凝集性能

用绵羊、家鸡、麻鸭、家鸽、家兔、白鼠、牛蛙及人的A、B、AB、O型血共11种血细胞对福建常见的11种软体动物进行了凝集素的筛选实验，结果表明，91％的动物粗蛋白提取液至少能凝集2种以上红细胞，绵羊红细胞对软体动物凝集素最敏感，同时发现实验动物凝集素与绵羊红细胞发生凝集反应的适宜pH为6.5-8.0，而其对高温的耐受性则各不相同，其中河蚬、菲律宾蛤仔、短蛸的凝集素具有较高的热稳定性，在95℃，15min处理后仍有活性。各种动物凝集素的凝集性能可以分别被不同的糖所抑制，但均可被0.02mol/L EDTA抑制。     

淡水育珠蚌血清凝集素的研究

在两种淡水育珠蚌（三角帆蚌和背角无齿蚌）血清中发现有凝集素存在,三角帆蚌的血清凝集素能凝集鸡、鸭和兔的红细胞,在ｐＨ５～９的范围内保持活性, 凝集活性能被甘露糖抑制,对Ｃａ＾２＋没有表现出明显的依赖性,背角无齿蚌的血清凝集素能凝集鸡和鸭的红细胞,但不能凝集兔的红细胞,在ｐＨ６～９的范围内保持活性,其凝集活性对Ｃａ＾２＋有明显的依赖性,而且可被Ｎ－乙酰葡萄糖胺强烈抑制,两者的血清经６０℃加热处理３０     

泥蚶(Tegillarca granosa Linnaeus)凝集素的细胞凝集和糖抑制作用的研究

泥蚶(TegillarcagranosaLinnaeus)凝集素能凝集所测试的14种人或动物的红细胞,其中对兔的红细跑的凝集活性最高.泥蚶凝集素也能凝集大鼠和小鼠的肺、脾脏、肝脏、骨髓细胞以及小鼠精子细胞.该凝集素对兔的红细胞的凝集活性不被测试的10种浓度为200mmol·L-1的糖所抑制.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.