指示剂法快速检测结核杆菌耐药试验的探讨

结核杆菌快速耐药试验方法学的研究，是结核病细菌学急待解决的问题。国内经典的检测方法都存在着各自的优缺点，而未能改变现行改良罗氏培养耐药试验方法[1]。因此我们探讨用指示剂快速检测结核杆菌耐药试验的方法。1材料与方法1．12％B指示剂1．2含药培养基以舒通培养基为基础，制成液体培养基，经103．43KPa，75％乙醇处理后，15分钟高压灭菌加入药物，然后分装于15mm×10mm小试管中，每管吸1．0ml，其中药物含量：SM：100μg／ml10μg／mlINH：10μg／ml1μg／mlPAS：10μg／ml1μg／ml25μg／ml5μg／mlKM：50μg／ml10μg／mlEM…     

化工区成年人体内β_2-m含量观察

本文对416例化工区居住5年以上者进行了血清β_2－m含量测定，其结果观察组为2．11±0．48μg/ml；对照组为1．96±0．51μg/ml（P＜0．01）。另外观察组与对照组之间，男性为2．14±0．51μg/ml；1．95±0．477μg/ml（P＜0．01），女性为2．0±0．46μg/ml，1．98±0．54μg/ml（＜0．05）。实验结果表明，化工区成年人机体内β_2－m含量有不同程度的改变。     

催化动力学光度法测定痕量铁-Fe(Ⅲ)-H_2O_2-茜素紫3B-Phen体系

本文研究了在HAc－NaAc介质中，邻菲啉活化Fe(Ⅲ)催化H2O2氧化茜素紫3B的褪色反应，建立了催化动力学光度法测定痕量铁的新方法．本方法的测定范围为0－20μg／25ml，检出限为8.5×10-11g／ml，用于铝合金和水中铁的测定，结果满意．     

镱（Ⅲ）与2—（5—溴—2—吡啶偶氮）—5—二乙氨基酚显色反应的分光光度法研究

研究了在表面活性剂CTMAB存在下镱（Ⅲ）与5－Br－PADAP的显色反应．结果表明，在pH=9．66的NH4Cl－NH3·H2O缓冲溶液中，镱（Ⅲ）与5－Br－PADAP进行最佳络合．最大吸收波长λmax=580nm．表观摩尔系数为1．5×105L·mol-1·cm－1，镱（Ⅲ）星在0－12μg／25ml范围内符号比尔定律．     

萃取催化动力学光度法测定痕量锰

研究了在pH9．7的介质中,利用Mn（2＋）催化过氧化氢氧化水杨醛显色的指示反应,用萃取平衡控制反应时间及反应程度,通过测量500nm下水相的吸光度,建立了萃取催化光度法测定痕量锰的新方法方法的线性范围为0．020－0．20μg／ml,检出限为0．010μg／ml用于水样中痕量锰的测定,结果满意。     

丁基罗丹明B—溴酸钾—溴代十六烷基吡啶体系催化光度法测定痕量钒的研究

本文研究了在柠檬酸介质中,有溴代十六烷基吡啶存在时钒催化溴酸钾氧化丁基罗丹明B的褪色反应,建立了催化光度法测定痕量钒的新方法．本方法的线性范围为0．010－0．200μg／25ml,检出限为13×10－10g／ml．用于岩石中痕量钒的测定,结果满意．     

4种软体动物凝集素的细胞凝集性研究

从4种软体动物肌肉中获得凝集素粗提液．用人的四种血型红细胞和三种动物红细胞检测其凝集活性，结果表明，每种软体动物凝集素至少凝集6种以上的红细胞．兔血红细胞对凝集素最敏感．能够和兔血细胞发生凝集反应的海螺丝、缢蛙、毛蚶、四角蛤蜊凝集素提取液的最小蛋白浓度分别为0．67μg／ml，0.78μg／ml，0．29μg／ml和0．49μg/ml。6种微生物细胞或孢子不能与凝集素样品发生凝集反应。     

诱导动力学光度法测定微量硒的研究

本文研究了Se（IV）对Cr（VI）－I－－淀粉氧化还原反应体系的诱导作用，以此为基础提出了一种测定微量硒的动力学新方法，建立了测定的最佳条件。Se（IV）浓度在0～52．5μg／25ml范围内与诱导──—非诱导体系的吸光度差成正比，方法的检测限为0.02μg／ml。用本法测定了土壤中微量硒的含量，结果满意。     

大鼠血浆中芦丁和槲皮素的RP-HPLC同时分析方法

建立测定大鼠血浆中芦丁和槲皮素含量的反相高效液相色谱(RP—HPLC)方法．用甲醇-乙酸(9:1, v／v)去血浆样品中蛋白质处理,以香兰素作内标,采用Century SIL C18BDS(250 mm×4．6 mm,5μm)色谱柱, 以甲醇-水-磷酸(45:53:0．02)为流动相,流速为0．7 ml／min;紫外检测波长为370nm,柱温为室温,进样量 10 μl．芦丁的定量限为0．068 μg／ml,线性范围为0．068～8．6 μg／ml;槲皮素的定量限为0．0502μg／ml,线性范围为0．0502～13μg／ml．芦丁和槲皮素的平均回收率分别为95．3％,RSD=4．1和90．3％,RSD=2．53(n =6),芦丁的日内及日间精密度的相对标准差分别小于13．2％和9．9％,而槲皮素的日内及日间精密度的相对标准差分别为小于13．9％和11．3％．该方法简单、快速、灵敏度高、重复性良好,可作为芦丁和槲皮素体内样品的分析方法．     

高效液相色谱法测定样品中紫杉醇的含量

建立了反相高效液相色谱法测定样品中紫杉醇含量的方法。色谱柱采用中国科学院大连化学物理研究所生产的YWQC18柱（4．6X250mm,5μm）,流动相为乙腈：水：三氟乙酸（40：60：0．1）,检测波长227um,紫杉醇浓度在40μg／ml～1400μg／ml内呈解性关系。回收率98．2～99．3％,变异系数0．75～3．39％。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.