| 小麦幼叶中小G蛋白的粗分离 |
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| 引用本文： | 张秀峰,高丽美,韩榕.小麦幼叶中小G蛋白的粗分离[J].山西师范大学学报,2010(4). |
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| 作者姓名： | 张秀峰 高丽美 韩榕 |
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| 作者单位： | 山西师范大学生命科学学院; |
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| 基金项目： | 山西省自然基金项目(2008011059-1); 山西省研究生优秀创新项目(104029) |
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| 摘 要： | 对小麦幼苗叶片中的蛋白进行了粗提,聚丙烯酰胺凝胶电泳(SDS-PAGE)检测在20.1 KDa～31 KDa间呈现一条蛋白条带,经分析其分子量约为23.1 KDa,占所提取总蛋白的2.93%,推测其为小G蛋白家族中的成员.采用传统的硫酸铵盐析法对此目的蛋白进行粗分离.结果显示,30%的硫酸铵饱和度可将该蛋白分离,且所含杂蛋白量最少,其纯度和得率分别是22.96%和47.3%,是进一步细分离纯化的较好选择.
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| 关 键 词： | 盐析纯化 SDS-PAGE 小G蛋白 小麦 |
The Crude Isolation of Small G Protein from Young Leaves of Wheat |
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| ZHANG Xiu-feng,GAO Li-mei,HAN Rong.The Crude Isolation of Small G Protein from Young Leaves of Wheat[J].Journal of Shanxi Teachers University,2010(4). |
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| Authors: | ZHANG Xiu-feng GAO Li-mei HAN Rong |
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| Institution: | ZHANG Xiu-feng,GAO Li-mei,HAN Rong(School of Life Science,Shanxi Normal University,Linfen 041004,Shanxi,China) |
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| Abstract: | The proteins in wheat seedling leaves was crudely extracted.SDS-PAGE clearly revealed a protein band between 20.1 KDa and 31 KDa,its molecular weights was about 23.1 KDa,contented 2.93% of total protein.We sepculated that it belongs to the family of small G protein.The salt precipitation assay was used to primary separate this protein as objective protein.The results showed that this protein can be separated by 30% saturation of ammonium sulfate,containing the least other proteins,the yieldrate is 47.3% and... |
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| Keywords: | salting-out purification SDS-PAGE small G protein wheat |
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