| Microbial Transformation of Acetophenone to 2—Phenylethanol |
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| 引用本文： | 蔺兆星,郝宁,陈国强.Microbial Transformation of Acetophenone to 2—Phenylethanol[J].清华大学学报,2003,8(4):471-475. |
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| 作者姓名： | 蔺兆星 郝宁 陈国强 |
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| 作者单位： | DepartmentofBiologicalSciencesandBiotechnology,TsinghuaUniversity,Beijing100084,China |
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| 基金项目： | Supported by the National Natural Science Foundation of China(No.2 0 0 74 0 2 0 ) |
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| 摘 要： | Microorganisms are commonly used to transform chemicals to chiral compounds. Pseudomonas cepacia 1813, Pseudomonas stutzeri 1317, Saccharomyces cerev isiae 1912, and 5 Escherichio coil strainswere used to transform acetophenone to 2-phenylethanol. The results show that the E. coli strains have the poorest biotransformation ability. P. stutzeri 1317 provides the best transformation ability with a transformation rate of 30% achieved with either whole or broken P. stutzeri 1317 cells for a reaction pH of 8. 2 and an initial acetophenone concentration of 2 g/L. An acetophenone concentration of 10 g/L strongly inhibits the biotransformation for a pH of 8. 2. Crude alcohol dehydrogenase obtained from S. cerevisiae 1912 transforms acetophenone to 2-phenylethanol when nicotinamide adenine dinucleotide reduced (NADH) is added.
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| 关 键 词： | 苯乙酮 2－苯基乙醇 微生物 生物转化 |
Microbial Transformation of Acetophenone to 2-Phenylethanol |
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| LIN Zhaoxing,HAO Ning,CHEN Guoqiang.Microbial Transformation of Acetophenone to 2-Phenylethanol[J].Tsinghua Science and Technology,2003,8(4):471-475. |
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| Authors: | LIN Zhaoxing HAO Ning CHEN Guoqiang |
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| Institution: | LIN Zhaoxing,HAO Ning,CHEN Guoqiang ** Department of Biological Sciences and Biotechnology,Tsinghua University,Beijing 100084,China |
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| Abstract: | Microorganisms are commonly used to transform chemicals to chiral compounds. Pseudomonas cepacia 1813, Pseudomonas stutzeri 1317, Saccharomyces cerevisiae 1912, and 5 Escherichia coli strains were used to transform acetophenone to 2 phenylethanol. The results show that the E. coli strains have the poorest biotransformation ability. P. stutzeri 1317 provides the best transformation ability with a transformation rate of 30% achieved with either whole or broken P. stutzeri 1317 cells for a reaction pH of 8.2 and an initial acetophenone concentration of 2 g/L. An acetophenone concentration of 10 g/L strongly inhibits the biotransformation for a pH of 8.2. Crude alcohol dehydrogenase obtained from S. cerevisiae 1912 transforms acetophenone to 2 phenylethanol when nicotinamide adenine dinucleotide reduced (NADH) is added. |
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| Keywords: | acetophenone 2 phenylethanol biotransformation Pseudomonas stuzteri |
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