| Extraction and analysis of polyunsaturated fatty acid from Asterina pectinifera in Huanghai Sea |
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| 作者姓名： | 郭承华 Liu Wanshun Han Baoqin Dong Xinwei Liu Chuanlin Hu Dan Soon-Teck Jung |
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| 作者单位： | [1]College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P.R. China [2]Scientific and Technological College of Chemistry and Biology, Yantai University, Yantai 264005, P. R. China [3]Food Industrial Technology Center of Mokpo University, Mokpo 534-72, Korea |
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| 摘 要： | The full fats of Asterina pectinifera in Huanghai Sea were extracted and refined using solvent extraction combined with silica gel colmnn chromatography with yield of 1.64%. The full fats were analyzed by gas chromatography and the result indicated that the full fats from Asterina pectinifera contained abundant Polyunsaturated Fatty Acid （PUFA） with a total of 25 kinds, especially rich in EPA and DHA. After enrichment by silica gel colmnn chromatography, the total PUFA content in ligarine fraction is 42.20%, in which EPA and DHA account for 12.50% and 10.33% respectively. The total PUFA content in acetic ether fraction is 48.98%, in which EPA and DHA take up 17.53% and 6.59% respectively. The total amount of EPA and DHA in both fractions all exceeded that of the fish oil from deep sea. In conclusion, the Asterina pectinifera in Huanghai Sea is a favorable source of PUFA.
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| 关 键 词： | PUFA EPA DHA 萃取 凝胶 气相色谱分析 不饱和脂肪酸 |
| 收稿时间： | 2005-03-15 |
Extraction and analysis of polyunsaturated fatty acid from Asterina pectinifera in Huanghai Sea |
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| Guo Chenghua,Liu Wanshun,Han Baoqin,Dong Xinwei,Liu Chuanlin,Hu Dan,Soon-Teck Jung.Extraction and analysis of polyunsaturated fatty acid from Asterina pectinifera in Huanghai Sea[J].High Technology Letters,2006,12(2):198-202. |
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| Authors: | Guo Chenghua Liu Wanshun Han Baoqin Dong Xinwei Liu Chuanlin Hu Dan Soon-Teck Jung |
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| Abstract: | The full fats of Asterina pectinifera in Huanghai Sea were extracted and refined using solvent extraction combined with silica gel column chromatography with yield of 1.64 %. The full fats were analyzed by gas chromatography and the result indicated that the full fats from Asterina pectinifera contained abundant Polyunsaturated Fatty Acid (PUFA) with a total of 25 kinds, especially rich in EPA and DHA. After enrichment by silica gel column chromatography, the total PUFA content in ligarine fraction is 42.20%, in which EPA and DHA account for 12.50% and 10.33% respectively. The total PUFA content in acetic ether fraction is 48.98%, in which EPA and DHA take up 17.53% and 6.59% respectively. The total amount of EPA and DHA in both fractions all exceeded that of the fish oil from deep sea. In conclusion,the Asterina pectinifera in Huanghai Sea is a favorable source of PUFA. |
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| Keywords: | Asterina pectinifera PUFA EPA DHA extraction silica gel column chromatography gas chromatography |
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