|
|||||
|
|
| Isolation and characterization of rice lesion mimic mutants from a T-DNA tagged population | |
| 作者姓名： | LI Shutian PEI Zhongyou LUO Lijuan TIAN Yingchuan HE Chaozu |
| 作者单位： | National Key Laboratory of Plant Genomics, Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China;Graduate School of the Chinese Academy of Sciences, Beijing 100039, China,National Key Laboratory of Plant Genomics, Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China,College of Agronomy, South China University ofTropical Agriculture, Danzhou 571737, China,National Key Laboratory of Plant Genomics, Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China,National Key Laboratory of Plant Genomics, Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | A rice ( Oryza sativa L. ssp. japonica cv. Nipponbare) T-DNA tagged population consisting of about 7000 individual lines was generated and screened for rice lesion mimic mutants in the T1 generation. Ten lines were found to develop spontaneous lesions in the absence of pathogen infection and displayed distinct lesion phenotypes. These mutants were tentatively designated as lm1 -lm10 (for lesion mimic), respectively. Lesion formation of lm mutants was developmentally regulated, and all the mutants showed stunted growth and reduced fertility. Genetic analysis demonstrated that all the mutations were recessive, and five partially fertile mutants (lm4-lm8) were derived from different loci. Mimic lesions occurring on the leaves of lm mutants resulted from cell death as revealed by trypan blue staining. Six of them ( lm3 -lm8 ) exhibited enhanced resistance to five bacterial blight isolates, indicating their wide-spectrum resistance to this pathogen. These results imply that some lesion mimic mutations of rice might be involved in disease resistance signaling pathways,and that isolation of these mutated genes may be useful for elucidating molecular mechanisms of plant disease resistance. Among the mutants, only one mutant, lm6, was preliminarily shown to cosegregate with the inserted T-DNA in its T1 generation, making it feasible to isolate the gene responsible for the phenotype of this mutant. |
| 关 键 词： | T-NDA tagging lesion mimic hypersensitive response Xanthomonas oryzae pv. Oryzae Oryza sativa |
Isolation and characterization of rice lesion mimic mutants from a T-DNA tagged population |
|
| LI Shutian,PEI Zhongyou,LUO Lijuan,TIAN Yingchuan,HE Chaozu.Isolation and characterization of rice lesion mimic mutants from a T-DNA tagged population[J].Progress in Natural Science,2005,15(1):17-23. | |
| Authors: | Li Shutian PEI Zhongyou Luo Lijuan TIAN Yingchuan HE Chaozu |
| Institution: | 1. National Key Laboratory of Plant Genomics, Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China;Graduate School of the Chinese Academy of Sciences, Beijing 100039, China 2. National Key Laboratory of Plant Genomics, Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China 3. College of Agronomy, South China University ofTropical Agriculture, Danzhou 571737, China |
| Abstract: | A rice ( Oryza sativa L. ssp. japonica cv. Nipponbare) T-DNA tagged population consisting of about 7000 individual lines was generated and screened for rice lesion mimic mutants in the T1 generation. Ten lines were found to develop spontaneous lesions in the absence of pathogen infection and displayed distinct lesion phenotypes. These mutants were tentatively designated as lm1 -lm10 (for lesion mimic), respectively. Lesion formation of lm mutants was developmentally regulated, and all the mutants showed stunted growth and reduced fertility. Genetic analysis demonstrated that all the mutations were recessive, and five partially fertile mutants (lm4-lm8) were derived from different loci. Mimic lesions occurring on the leaves of lm mutants resulted from cell death as revealed by trypan blue staining. Six of them ( lm3 -lm8 ) exhibited enhanced resistance to five bacterial blight isolates, indicating their wide-spectrum resistance to this pathogen. These results imply that some lesion mimic mutations of rice might be involved in disease resistance signaling pathways,and that isolation of these mutated genes may be useful for elucidating molecular mechanisms of plant disease resistance. Among the mutants, only one mutant, lm6, was preliminarily shown to cosegregate with the inserted T-DNA in its T1 generation, making it feasible to isolate the gene responsible for the phenotype of this mutant. |
| Keywords: | T-DNA tagging lesion mimic hypersensitive response Xanthomonas oryzae pv Oryzae Oryza sativa |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《自然科学进展(英文版)》浏览原始摘要信息 | |
| 点击此处可从《自然科学进展(英文版)》下载免费的PDF全文 | |