双载体固定化中国红豆杉细胞的初步研究

对采用海藻酸钠和聚乙烯醇（ＰＶＡ）混合的双勒体包埋中国红豆杉悬浮细胞的条件进行了初步研究，建立了一套合适的国家化细胞条件，海藻酸钠浓度为３０ｇ／Ｌ，聚乙烯醇（ＰＶＡ）浓度为７０ｇ／Ｌ，ＣａＣｌ２浓度２０／Ｌ，包埋浓度为０．１８ｇ／Ｌ，接种密度为０．１２ｇ／Ｌ，在此基础上，对中国红豆杉细胞固定化２的培养基进行了优化，实验结果表明，从红豆杉树皮中分离出的真菌物作为诱导物能限制细胞生长，且明显促进紫杉醇

Taxol Production by Immobilized Cultures of Taxus Chinesis Suspensis Cell

By studying the conditions of entrapping Taxus chinesis suspense cells with sodium alginate, the suitable immobilizing operation conditions have been established as that the concentration of sodium alginate is 30 g/L, the concentration of polyvinyl alcohol is 70 g/L, the concentration fo calcium chloride is 20 g/L, the trapped cells density is 0.18 kg/L and the inoculations are 0.12 kg/L. On the basis of the above study, the media for immobilized cells of Taxus chiensis are optimized. The experimental results show that the elicitor of the fungi which is screend from Taxus chinensis bark can restrict the cell growth, improve significantly taxol yield and promote excretion of taxol into the medium, and that proper addition of the precursors can also promote the synthesis of taxol.