| 南瓜属4个栽培种种子蛋白及电泳图谱的比较 |
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| 引用本文： | 黄秀丽,庄东红,彭桂庄,曲莹,林奕韩.南瓜属4个栽培种种子蛋白及电泳图谱的比较[J].汕头大学学报(自然科学版),2005,20(3):54-59. |
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| 作者姓名： | 黄秀丽 庄东红 彭桂庄 曲莹 林奕韩 |
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| 作者单位： | [1]汕头大学生物系,广东汕头515063 [2]汕头市白沙蔬菜原种研究所,广东汕头515800 |
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| 基金项目： | 广东省科技计划资助项目（No：2002A2070602） |
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| 摘 要： | 对南瓜属4个栽培种中的11个品种进行种子蛋白质含量及碱溶蛋白SDS-PAGE分析.结果显示,蛋白质含量在4栽培种间呈现一定的差异,在种内品种间则比较接近;非还原与还原条件下,4栽培种间蛋白谱带均有差异,可将各栽培种区别开,其中以还原条件下区别更为明显.栽培品种日本南瓜同时显示出中国南瓜和印度南瓜的特征条带,推测可能是2个种的种间杂交种.
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| 关 键 词： | 南瓜 种子总蛋白 碱溶蛋白 SDS-PAGE |
| 文章编号： | 1001-4217（2005）03-0054-06 |
| 收稿时间： | 2005-04-05 |
| 修稿时间： | 2005年4月5日 |
Comparison of Content and Electrophoretic Pattern of Sccd Proteins of Four Cultivated Species in the Genus Cucurbita |
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| Huang XiuLi;Zhuang DongGong;Peng GuiZhuang;Qu Ying;Lin YiHan.Comparison of Content and Electrophoretic Pattern of Sccd Proteins of Four Cultivated Species in the Genus Cucurbita[J].Journal of Shantou University(Natural Science Edition),2005,20(3):54-59. |
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| Authors: | Huang XiuLi;Zhuang DongGong;Peng GuiZhuang;Qu Ying;Lin YiHan |
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| Abstract: | The content of seed proteins and the electrophoretic pattern of alkali-soluble seed proteins are analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)of 11 cultivaters from four cultivated species in genus Cucurbita.The results showed:Seed proteins contents were different among four cultivated species,and they were more similar among cultivaters of one cultivated specie;Under no-reducing and reducing conditions,the protein pattern showed difference among four cultivated species,and it was more obvious under reducing condition;Under no-reducing condition,there were some differences of protein pattern among cultivaters of one cultivated specie.The cultivater Riben Nangua showed the characteristic protein bands of C.moschato Duch and C.maxima Duch at the same time.It is conferred that it was the crossbreed of this two cultivated species. |
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| Keywords: | pumpkin seed protein alkali-soluble protein sodium dodeeyl sulfate-polyacrylamide gel electrophoresis |
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