非直接荧光免疫法鉴定HMG＿（14）非组蛋白质在果蝇唾腺染色体上的原位分布

以获得的抗ＨＭＧ＿（１４）ＭｃＡｂ为探针,运用间接荧光免疫技术,对果蝇（Ｄ,Ｖｉｖｉｌｉｓ南京）唾腺染色体上ＨＭＧ＿（１４）的分布及其ＨＭＧ＿（１４）与活性区“ｐｕｆｆ”的关系进行了初步观察。发现;（１）ＨＭＣ＿（１４）分布在唾腺染色体所有的横纹带上;（２）在“ｐｕｆｆ”区,ＨＭＧ＿（１４）有增加的趋势;（３）ＤＮａｓｅＩ处理可以使染色体上的ＨＭＧ＿（１４）丢失。实验结果进一步验证了ＨＭＧ＿（１４）是染色体上的一种结构性蛋白。

Indirect Immnnofinorescent Method for Determinationof the in Situ Distribution of HMG_(14) Nonhiston Proteinof Drosophila Salivary Chromosome

A technique has been developed for "staining" cytological preparation by indirectimmunofluorescent methods that Permits determination of the in situ disiribution ofchromosomal proteins. The method is Particularly oriented to use of polytene chromosomesqnashes from Drosophila salivary gland. The results obtained demonstrate the specificin vivo chromosomal locations of nonhiston protein HMG purified froiri isolated chroma-tin. The fluorescent patterns that we have observed are: (1) All bands are stained, butquantitative differences in the fluorescence of particular bands are apparent. (2) Mostpuffs are brighty stained. The chromocenter is brightly stained. (3) After DNase Idigested, all fluorescent bands are losted. ( 4 ) The traditional technique yields the samequalitative fluorescent patterns.