北五味子粗多糖对酒精诱导小鼠急性肝损伤的保护作用及其机制研究

目的观察北五味子粗多糖（SCP）对酒精诱导小鼠急性肝损伤的保护作用,探讨其作用机制.方法采用50%乙醇12 mL/kg灌胃建立小鼠急性肝损伤模型,检测小鼠肝质量指数,血清中转氨酶活性（ALT和AST）和甘油三脂（TG）水平,肝组织中丙二醛（MDA）、还原型谷胱甘肽（GSH）含量、一氧化氮合酶（NOS）活性以及HE染色观察肝脏病理学改变.结果北五味子粗多糖可明显降低小鼠肝质量指数,血清ALT,AST以及TG水平（P〈0.05）,同时使小鼠肝组织MDA水平和NOS活性显著下降,GSH水平升高（P〈0.05或P〈0.01）,并可改善小鼠肝脏病理损伤.结论 SCP具有明显的肝保护作用,机制可能与抗氧化有关.

Protective Effect of Schisandra chinensis Polysaccharide on Acute Liver Injury Induced by Alcohol in Mice and Its Mechanism

Objective To investigate the protective effect of Schisandra chinensis polysaccharide（SCP） on liver injury and explore its mechanism. Methods An acute alcoholic liver injury model in mice was induced,and the liver weight index,alanine aminotransferase（ALT） and aspartate aminotransferase（AST） activities,triglyceride （TG） level in serum,malondialdehyde（MDA）,reduced glutathione（GSH） contents,and nitric oxide synthase （ NOS） activity in the liver tissues were tested. HE staining was performed for observing pathological changes of the liver tissues. Results SCP significantly decreased liver index of mice,ALT,AST activities and TG levels in serum（P〈0. 05）. Meanwhile,the leve of MDA contents and NOS activities in the liver tissues were reduced significantly,and GSH levels in the liver tissues were increased by SCP（P〈0. 05 or P〈0. 01）. Moreover,SCP significantly reduced pathological injury of liver cells. Conclusion SCP had a significant protective effect on alcohol-induced acute liver injury,which might be related to its anti-oxidant activity.