| 基于16S rDNA序列和RFLP分析的病鳗分离菌株鉴定 |
| |
| 引用本文： | 郭松林,关瑞章,冯建军,杨求华.基于16S rDNA序列和RFLP分析的病鳗分离菌株鉴定[J].集美大学学报(自然科学版),2012(2):81-88. |
| |
| 作者姓名： | 郭松林 关瑞章 冯建军 杨求华 |
| |
| 作者单位： | 集美大学水产学院;教育部鳗鱼产业技术工程研究中心 |
| |
| 基金项目： | 国家自然科学基金(31001136);农业部公益性行业科研专项(3-51);福建省教育厅项目(JA10191);福建省教育厅资助省属高校科研专项(JK2011029) |
| |
| 摘 要： | 结合16S rDNA基因序列和限制性片段长度多态性(RFLP)的分析方法,通过与GenBank库中已递交的细菌16S rDNA基因序列进行同源性比较,对分离自发病鳗鲡(欧洲鳗鲡,日本鳗鲡和美洲鳗鲡)的30株细菌进行初步鉴定和分类.结果表明,这些细菌可大致分为气单胞菌属(Aeromonas sp.)、假单胞菌属(Pseudomonas sp.)、邻单胞菌属(Plesiomonas sp.)、克雷伯氏菌属(Klebsiella sp.)、柠檬酸杆菌属(Citrobacter sp.)、不动杆菌属(Acinetobacter sp.)和肠杆菌属(Enterobacter sp.)等7个菌属.分析认为,部分分离菌株可能是引起鳗鲡病害的疑似致病性菌株.
|
| 关 键 词： | 鳗鲡 细菌 16S rDNA RFLP 鉴定 |
Identification of Bacteria Isolated from Diseased Eels Using 16S rDNA PCR\RFLP Analysis |
| |
| GUO Song-lin,GUAN Rui-zhang,FENG Jian-jun,YANG Qiu-hua.Identification of Bacteria Isolated from Diseased Eels Using 16S rDNA PCR\RFLP Analysis[J].the Editorial Board of Jimei University(Natural Science),2012(2):81-88. |
| |
| Authors: | GUO Song-lin GUAN Rui-zhang FENG Jian-jun YANG Qiu-hua |
| |
| Institution: | 1,2(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Engineering Research Center of Eel Industrial Technology,Ministry of Education,Xiamen 361021,China) |
| |
| Abstract: | Using 16S rDNA gene sequences and restriction fragment length polymorphism(RFLP)analysis,the study initially identified 30 strains of bacteria isolated from European eel(Anguilla anguilla),Japanese eel(Anguilla japonica)and American eel(Anguilla rostrata)according to the homology comparison with bacterial 16S rDNA gene sequence which had been submitted into the GeneBank database.The results showed that the 30 bacteria could be broadly divided into 7 genera,including Aeromonas sp.,Pseudomonas sp.,Plesiomonas sp.,Klebsiella sp.,Citrobacter sp.,Acinetobacter sp.and Enterobacter sp.Results of the study indicated some of the isolates could be suspected as pathogenic strains which caused diseases to eels. |
| |
| Keywords: | eel bacteria 16S rDNA RFLP identification |
| 本文献已被 CNKI 等数据库收录！ |
|
