排序方式: 共有38条查询结果,搜索用时 46 毫秒
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以HMGR酶基因为研究对象,采用实时荧光RT-PCR技术,研究了Couette式反应器中0.3 Pa层流剪切力作用后悬浮培养的对数期东北红豆杉细胞HMGR酶基因转录情况.结果显示,在对数生长时期,未经剪切处理的悬浮培养虹豆杉细胞HMGR基因转录水平呈增长趋势,而剪切处理后的悬浮培养红豆杉细胞HMGR基因转录水平则基本保持不变.同时,对相应细胞生物量的测定结果也显示出类似的变化.以上结果说明在对数生长时期施加一定强度的剪切力,将使细胞生长发生停滞,谊生长停滞现象可能与因剪切所引起的HMGR基因转录水平下降有一定相关性. 相似文献
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本文研究了用对-氨基苯砜乙基(ABSE)-交联琼脂糖做载体,通过戊二醛共价交联,对醇脱氨酶、乳酸脱氢酶、辅酶Ⅰ进行了位置对位置的共固定化。按本实验方法得到的固定化三元全酶系统的辅酶再生能力为相应游离酶系统的5倍。本文还对该三元全酶系统的固定化机理和辅酶再生性能做了一定的讨论。 相似文献
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本文报道测定辅酶A(CoA)活力的简化磺胺乙酰化法。该法用鲜鸽肝酶液代替鸽肝丙酮粉,设计了一简单装置贮存鲜酶液,简化了操作程序,测定准确,重现性好。 相似文献
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Summary Galactosamine, a selective hepatotoxin, produces in rats histologic alterations, which show the characteristics of severe human viral hepatitis. In the present study the efficacy of two different cofactor regimens (coenzyme A, NAD, alpha lipoic-acid, cocarboxylase) in rats with fulminant galactosamine hepatitis were tested. The results showed an improvement of the short-term survival with a short-term treatment and definitely better survival with a long-term regimen with cofactors. 相似文献
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S. Padilla U. C. Tran M. Jiménez-Hidalgo J. M. López-Martín A. Martín-Montalvo C. F. Clarke P. Navas C. Santos-Ocaña 《Cellular and molecular life sciences : CMLS》2009,66(1):173-186
Coenzyme Q is a lipid molecule required for respiration and antioxidant protection. Q biosynthesis in Saccharomyces cerevisiae requires nine proteins (Coq1p–Coq9p). We demonstrate in this study that Q levels are modulated during growth by its conversion
from demethoxy-Q (DMQ), a late intermediate. Similar conversion was produced when cells were subjected to oxidative stress
conditions. Changes in Q6/DMQ6 ratio were accompanied by changes in COQ7 gene mRNA levels encoding the protein responsible for the DMQ hydroxylation, the penultimate step in Q biosynthesis pathway.
Yeast coq null mutant failed to accumulate any Q late biosynthetic intermediate. However, in coq7 mutants the addition of exogenous Q produces the DMQ synthesis. Similar effect was produced by over-expressing ABC1/COQ8. These results support the existence of a biosynthetic complex that allows the DMQ6 accumulation and suggest that Coq7p is a control point for the Q biosynthesis regulation in yeast.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Received 04 September 2008; received after revision 22 October 2008; accepted 23 October 2008 相似文献
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几种N—芳基—exo—3,6—环氧—1,2,3,6—四氢酞酰胺酸的核磁共振研究 总被引:1,自引:1,他引:0
我们对六种N-芳基-exo-3,6-环氧-1,2,3,6-四氢酞酰胺酸及其与不同类型的亲核试剂反应生成的产物进行了^1H核磁共振谱的研究,测得^1H谱化学位移,研究探讨其规律性。并测试了IR谱和元素分析,确定了该类化合物的结构。 相似文献
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以电化学和光谱电化学方法探讨了合成辅酶B12模型化合物的条件。在此基础上用化学方法合成了系列轴向基团具有纯电子效应的辅酶B12模型化合物RPhCH_2-CoTMAPI(R=CH_(3-),NO_(2-),H-,Br-,KCO_(2-),COTMAP I为碘化四[N,N,N-三甲胺基]苯基钴卟啉)。以红外,紫外可见光谱对它们进行了表征。用量子化学方法讨论电子效应对Co-C键的影响。 相似文献
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Wan Jian 《华南师范大学学报(自然科学版)》2014,(6)
正Recently,docking has been widely used to predict the binding-modes of protein-inhibitors,when the crystal complexes structure was absent.Most docking algorithms are able to generate a large number of probable conformations,it,however,is difficult to effectively evaluate these docking poses and identify the most reasonable bindingmode.In the present study,on the basis of the crystallographic data of human 3-hydroxy-3-methylglutaryl coenzyme 相似文献