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The specific interaction between angiogenin and aptamer has been investigated by using AFM. The specificity of the interaction is revealed by comparing the binding probability of aptamer to other elements in a series of control experiments. The results have shown that there is specific interaction force between angiogenin and aptamer. Moreover, the single molecular pull-off force between angiogenin and aptamer has also been determined using the Poisson statistical method to be 133.7±11.7 pN. These findings obtained are helpful to the better revelation of recognition mechanism between angiogenin and aptamer, which provided basis for further understanding the inhibition of the aptamer to angiogenic activity. 相似文献
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利用RI-PCR方法从培养的人黑色素瘤细胞系A375中扩增得到了人血管生成素cDNA片段,测序正确后克隆入表达载体pET-28a( )中并转化于E.coli BL21宿主菌中.经IPTG诱导,表达了N端融合6个组氨酸标签(6His-tag)的血管生成素融合蛋白.利用6His-tag与过渡态金属离子Ni2 高亲和力结合的性质,经镍柱纯化,获得了高纯度的血管生成素融合蛋白,为进一步研究其生物活性及应用奠定了基础. 相似文献
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人血管生长素基因的化学合成与克隆 总被引:4,自引:0,他引:4
采用固相亚磷酸胺法化学合成并克隆人血管生长素基因,该基因全长423bp,其中369bp的结构基因全部选用大肠杆菌偏爱的密码子,在结构基因上游设置了适合原核非融合蛋白表达的核糖体结合位点的S-D序列。采用两级退火一步拼接法成功克隆全长基因,该基因适合在多种融合或非融合原核表达载体中表达。 相似文献
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