香蕉等七种植物材料中酪氨酸酶活性及其同工酶谱的比较研究

本文用Ｌ－多巴和邻苯二酚二种酶活力测定体系分别测定了香蕉、蘑菇，土豆、山芋、芋艿，蚕豆和苹果共七种植物材料中的酪氨酸酶活性，并用凝胶电泳活性染色法分析了上述七种植物材料中酪氨酸酶同工酶。结果表明，不问植物材料中酪氨酸酶活性有非常明显的差异；植物材料中存在酪氨酸酶同工酶，不同植物材料酪氨酸酶同工酶谱不相同。这提示了不同植物材料中酪氨酸酶活性及同工酶的差异可能与植物的组织结构和生理代谢有密切关系。     

水提法生产左旋多巴的工艺研究及优化

为研究开发一种新的环境友好的、从猫豆中提取左旋多巴（L-dopa）的生产工艺,用弱酸性水将猫豆磨成水浆,常温下用聚丙烯酰胺（PAM）沉淀去除提取液中的固体颗粒,过滤,滤液煮沸后冷却至室温,再次用PAM沉淀去除热变性析出的蛋白和淀粉,过滤,滤液浓缩,析出L-dopa粗品;粗品用热稀HCl精制,即可得到L-dopa产品;采用L₉（3⁴）正交试验法优选关键工序的工艺参数。结果表明：工艺不排放高含淀粉、蛋白、HCl和NaOH废水。试产3批,平均每批投料猫豆200 kg,L-dopa产品平均得率2.41%,平均含量99.84%,与传统工艺相当;含量和相关物质符合《中国药典》和《欧洲药典》要求。该生产工艺合理可行,绿色环保,适合于工业化规模生产。     

Dopamine regulation of testicular activity in intact and hypophysectomized frogs,Rana esculenta

In intact frogs, both GnRHA and L-dopa were able to increase testicular and plasma androgen levels and to induce spermiation. The dopamine antagonist pimozide inhibited both the effects of L-dopa but not those of GnRHa. Hypophysectomy reduced androgen levels, but spermiation was still induced by both GnRHa and L-dopa, suggesting that these agents can directly influence the testis through a route not involving the pars distalis. Again, pimozide antagonised spermiation induced by L-dopa but not that induced by GnRHa.This work was supported (40% and 60%) by the Italian Ministry of Education and the C.N.R. Special Project.