醚类汽油添加剂与L-02正常人肝细胞、人血红蛋白的体外作用研究

研究了新型无铅汽油添加剂甲基叔丁基醚（MTBE）、甲基叔戊基醚（TMAE）对正常人肝细胞系L－02的毒性作用，在较高浓度下，MTBE、TAME均对L－02正常人肝细胞的生长有影响，且TAME的毒性作用稍强，另外，用FT－IR法和^1H-NMR法研究了MTBE、TAME与人血红蛋白的体外作用，结果显示，MTBE、TAME与人血红蛋白无明显的直接作用。     

青春期女性血红蛋白正常参考值与中国地理因素

收集了中国76个单位用氰化高铁血红蛋白(HiCN)法测定的11 792例青春期女性血红蛋白正常参考值,运用相关分析和回归分析的方法,研究了其与地理因素的关系,发现青春期女性血红蛋白正常参考值与中国地理因素之间有很显著的相关关系(F=85.40).用逐步回归分析的方法推导出了一个回归方程:Y∧=120.7 0.005554X1 0.2460X3-0.8110X4±11.4如果知道了中国某地的地理因素,就可以用回归方程估算这个地区的血红蛋白正常参考值.依据血红蛋白正常参考值与地理因素的依赖关系把中国分为青藏区、西南区、西北区、东南区、华北区、东北区等六个区.     

反胶束溶液和血红蛋白水溶液相平衡的研究

研究了十六烷基三甲基溴化铵－正庚烷反胶束溶液和牛血红蛋白水溶液的相平衡,探讨了有机相助表面活性剂种类和浓度、水相ｐＨ值、水相离子种类和强度、表面活性剂浓度的影响,适宜条件下,牛血红蛋白的萃取率可达９５％以上。对实验结果从表面活性剂极性头与蛋白质分子间的相互作用和反胶团包溶蛋白质时的空间阻碍作用上进行了解释。     

无硝血红蛋白着色剂的研制

以新鲜猪血为原料,分离血清,依次进行防腐、发色和抗氧化处理,制成浆状的无硝血红蛋白着色剂。它的优良性能,经紫外－可见光光谱测定测定存放和光照实验,得到了验证。     

Effect of hydrogen peroxide on the binding of Merocyanine 540 to human erythrocytes

The fluorescent dye Merocyanine 540 (MC540) is often used as a probe to monitor the molecular packing of phospholipids in the outer leaflet of biomembranes. In a previous study we showed that the increased staining of erythrocytes with a perturbed membrane structure was mainly due to an increase in the fluorescence yield of cell-bound MC540, rather than to an increase of the number of bound molecules. Erythrocytes and ghosts exposed to continuous fluxes of H₂O₂ exhibited pronounced lipid peroxidation. Further, red blood cells subjected to this form of oxidative stress also showed increased staining with MC540. It appeared that this was caused by a strong increase in binding of MC540, together with a slight red shift of the fluorescence emission maximum and a small increase in the fluorescence yield of bound MC540. The changed MC540 binding characteristics were not observed when lipid peroxidation was suppressed by the presence of the antioxidant BHT in the incubation medium. However, open ghosts exposed to H₂O₂ showed no increase of MC540 binding, excluding a direct involvement of lipid peroxidation. Measurement of fluorescence emission spectra and gel filtration studies showed that MC540 can bind to H₂O₂-exposed hemoglobin. Experiments with erythrocytes lysed in hypotonic medium after exposure to H₂O₂ revealed that peroxidation of lipids with H₂O₂ induced a non-specific permeabilization of the plasma membrane to MC540, thereby allowing MC540 to bind to the oxidatively denatured, more hydrophobic hemoglobin. These results indicate that conclusions about packing of phospholipids in the outer leaflet of the membrane based on increased MC540-staining should be drawn with care. Received 27 September 1996; received after revision 5 November 1996; accepted 27 November 1996     

The strong cooperativity of dioxygen binding by dicobalt (II) diporphyrin compounds in nonaqueous solution at room temperature

Thermodynamic data were determined for the reversible binding of O₂ to two compounds of dicobalt(II) di(meso-tetra-phenyl)porphyrin derivatives with different lengths of diamidoaliphatic bridge (abbreviated to Co₂PP₈ and Co₂PP₄) in N,N-dimethylformamide at room temperature. The partial pressure of dioxygen necessary for half-oxygenation (P _1/2) and hill coefficient (n) at 298 K were determined as follows:P _1/2=54.2 kPa,n=2.0 for Co₂PP₈ andP _1/2=6.8 kPa,n=1.8 for Co₂PP₄, respectively. The rate equations of reversible oxygen binding by Co₂PP₈ were determined and the reaction path was proposed. The results of ther-modynamic and kinetic studies indicate that there exists strong cooperative effect during oxygenation of the compounds. The ESR observation reveals that the dioxygen complexes formed in the solutions are of superoxo (Co-O ₂ ⁻ ) type. Supported by the Research Foundation of the State Education Commission of China Zhou Xiaohai: born in 1962, Ph.D. Associate professor     

一种高灵敏度测定血红蛋白的化学发光分析方法

研究了在碱性介质和阳离子表面活性剂CTMAB存在下,牛血红蛋白催化鲁米诺-铁氰化钾的化学发光行为和化学发光反应的条件,提出一种流动注射-化学发光法测定牛血红蛋白的新方法.该方法测定牛血红蛋白的线性范围为5.18×10-10~1.29×10-6mol/L,r2=0.998 5;检出限(RSN=3)为1.2×10-10mol/L;回收率为(100±10)%;dRSD≤5%(n=6).     

我国中年女性血红蛋白的正常参考值与地理因素关系的研究

为制定中国中年女性血红蛋白正常参考值的统一标准提供科学依据.收集了中国268个单位用氰化高铁血红蛋白(HiCN)法测定的25917例中年女性血红蛋白正常参考值,运用相关分析和回归分析的方法,研究了其与地理因素的关系.结果发现中年女性血红蛋白正常参考值与中国地理因素之间有很显著的相关关系(F=212.88).用逐步回归分析的方法推导出了一个回归方程.根据中国某地的地理因素,用回归方程可以估算这个地区的血红蛋白正常参考值.依据血红蛋白正常参考值与地理因素的依赖关系把中国分为青藏区、西南区、西北区、东南区、华北区、东北区等六个区.     

运动疗法依从性对2型糖尿病患者糖化血红蛋白的影响

目的运动疗法依从性对2型糖尿病患者糖化血红蛋白的影响。方法2型糖尿病患者60例．出院后接受每月一次的电话回访。连续6个月，全部病人首次发病，进行运动疗法相关知识教育，每3个月查糖化血红蛋白。结果运动疗法依从性达标的患者糖化血红蛋白控制明显优于不能达标的患者（P〈0．05）。结论有效的运动可提高胰岛素敏感性。减轻胰岛素抵抗，对糖化血红蛋白有明显的控制作用。     

海拔高度对藏族儿童血红蛋白含量影响的调查分析

目的：了解居住在西藏不同海拔地区世居藏族儿童之间的血红蛋白含量，研究血红蛋白含量与高原海拔高度的关系。方法：对居住在海拔3700米（拉萨）的406名9—10岁藏族儿童和444名居住在海拔4300米（定日）的相同年龄段藏族儿童进行横向比较研究。采取随机抽样的方法，测量血红蛋白含量及人体基本数据测量，所得数据用SPSS14．0软件系统分析。结论：居住在海拔4300米的定日藏族儿童的血红蛋白含量14．0（13．9—14．1）g／dl明显低于（P〈0．05）居住在海拔3700米的拉萨藏族儿童的血红蛋白含量14．6（14．5—14．7）g／dl。