噬菌体展示禽流感病毒抗原变异性基因片段文库的构建

构建T7噬菌体展示禽流感病毒抗原变异性基因片段文库. 首先， 从Gene Bank中查找筛选禽流感病毒抗原变异性基因, 将其截短、 修饰、 简并后得到禽流感病毒抗原变异性基因微阵列. 其次， 将合成的禽流感病毒抗原变异性基因片段文库扩增、 酶切, 链接到双酶切后的T7噬菌体载体基因上, 构成重组噬菌体DNA. 最后， 重组噬菌体DNA经体外包装和扩增, 得到T7噬菌体展示文库, 并进行T7噬菌体展示文库滴度、 重组率和免疫活性测定. 实验结果表明, 从Gene Bank中查找、 筛选、 剪切和修饰共获得96 258条序列构建T7噬菌体展示文库, 原始文库滴度为3.6×107个菌落/mL, 重组率大于90%. 用禽流感病毒H5N1抗体进行捕获, 经聚合酶链式反应(PCR)鉴定, 得到理想目的条带, 证明噬菌体表面展示蛋白具有抗原活性, 可用于禽流感病毒感染患者的快速检测及抗原表位筛选.     

一种改进的基于效用理论的TOPSIS决策方法

提出了一种改进的TOPSIS决策方法.该方法更多地融合了决策者的主观意愿:用决策者对属性的效用评价代替方案的原始属性数据;用模糊数替代精确值反映决策者对效用值的理解模糊不确定性;同时关注决策者对最佳决策方案属性效用的综合性、均衡性考虑.最后,通过对一个具体决策实例的分析,验证了方法的合理性和有效性.     

基于机器视觉的车辆时空参数识别

车辆荷载信息是分析桥梁受力和性能评估的重要依据,其中车辆时空信息是准确识别车辆荷载的关键参数。考虑到传统在桥面埋设传感器的方法影响交通、破坏路面,提出了一种基于机器视觉技术的车辆荷载时空参数识别方法,包括车辆的横向定位、车速、车轴数、轴距的识别。基于正面拍摄视频,采用背景差分法来实现车辆检测,进一步通过车牌识别确定车辆在图像中的位置,然后根据车牌与车道线距离来实现车辆横向定位。基于侧面拍摄视频,采用图像标定方法,结合已知长度的辅助检测线来获取车辆的轴距、轴数、车速等车辆参数。通过试验室模型试验和现场试验验证了提出的方法。结果表明,现场试验中车辆横向定位和车速的最大误差分别为2.42%和2.67%,轴距的平均误差为1.63%,识别结果可以为更多其他车辆参数如轴重和总重的识别提供重要的技术支撑。     

Pattern formation in Rayleigh-Bénard convection: Numerical simulation with a coupled-map-lattice model

The various patterns formed in Rayleigh-Bénard convection under different conditions are numerically simulated with a coupled-map-lattice model. In the case of a lower aspect ratio (L/d=5), the simulations vividly depicted the main features of the transition process from a laminar state to a soft turbulence and then to a hard turbulence. In this case, the cellular structure in a horizontal section is also reproduced. In the case of a larger aspect ratio (L/d=30), the simulations successfully reproduced the spiral-defect chaos and target chaos in the initial stage of the pattern evolution which were recently found in experiments. In this case, for a fluid ofPr=1, it is verified in simulations that both the ideal straight rolls and spiral-defect chaos are stable attractors in the same parameter regime, and that the initial condition is a decisive factor for which one of the two is formed. In the same case, for a fluid ofPr=4, the target patterns are dominant instead of the spirals, the sizes and distributions of the target are also notably dependent on the initial condition.     

半刚性基层施工弯沉检测标准研究

根据重庆地区大量实测资料 ,对影响半刚性基层弯沉变异性的各主要因素进行了分析 ,并根据实际需要 ,对施工期实测弯沉的龄期与养生温度的影响进行了修正 ,得出可用于施工检测的弯沉控制标准     

台湾海区斜带石斑鱼群体遗传学的等位酶研究

应用聚丙烯酰胺凝胶和淀粉凝胶技术对台湾海区斜带石斑鱼的遗传结构和遗传多样性水平进行了等位酶分析。对18种酶系统共27个等位酶基因座位的检测表明。ADH—l、ODH-2、FDH-1和Est-3等5个基因座位具多态性，其群体的多态位点百分率P为18．5％。有效等位基因平均数Ne为1．185，平均杂合度的观测值Ho为0．0469，预期值He为0．0662，反映了该群体的遗传多样性水平在鱼类中处于中等程度，而其Hardy-Weinberg遗传偏离指数(D)为-0．246，表明该群体仍存在着明显的杂合子缺失现象，相对于捕捞压力，海岸线的工程改造是对其资源的更主要威胁。     

基于分形维数的心率变异分析

心率变异性（ＨＲＶ）分析是近年来发展起来的一种新的用于无创检测心脏植物神经功能的领域．目前已提出的各种心率变异指标在对心血管疾病的早期诊断、监护和预后评估中起了不可忽视的作用，特别是对心性猝死的预报和心肌梗死的预后判别等临床应用中有重要意义．理论分析和实验结果表明，心率信号具有近似分形的性质，是非线性的，因此，用分形维数表征心率变异性是符合逻辑的．文中作者在计算了多例心率信号的分形维数并考察了相应的功率谱的基础上，提出一种新的指标——分形维数对指标．此外，作者还提出了在低采样率心电数据中精确定位Ｒ峰的新算法，并且讨论了基于纽曼－皮尔逊准则的正常心动周期的判决方法     

A local positive feedback of the tropical Pacific ocean-atmosphere system on interdecadal timescales

SINCE THE 1990S, THE CLIMATIC VARIABILITY ON INTERDE- CADAL TIME SCALES BECAME THE FOCUS OF THE INTERNATIONAL CLIMATOLOGY RESEARCH MISSIONS[1―3]. ON TIME SCALES OF A DECADE OR MORE, THE OCEAN CIRCULATION PREDOMINATEDHEAT BALANCE AND HYDROLOGICAL CYCLE, S…     

Variability of surface circulation in the South China Sea from satellite altimeter data

11-year satellite altimeter sea surface height （SSH） anomaly data from January 1993 to December 2003 are used to present the dominant spatial patterns and temporal variations of the South China Sea （SCS） surface circulation through Empirical Orthogonal Function （EOF） analysis. The first three EOF modes show the obvious seasonal variations of SSH in the SCS. EOF mode one is generally characterized by a basin-wide circulation. Mode two describes the double-cell basin scale circulation structure. The two cells were located off west of the Luzon Island and southeast of Vietnam, respectively. EOF mode three presents the mesoscale eddy structure in the western SCS, which develops into a strong cyclonic eddy rapidly from July to September. EOF mode one and mode three are also embedded with interannual signals, indicating that the SCS surface circulation variation is influenced by El Nino events prominently. The strong El Nino of 1997/98 obviously changed the SCS circulation structure. This study also shows that there existed a series of mesoscale eddies in the western SCS, and their temporal variation indicates intra-seasonal and interannual signals.