Cancer and blood coagulation

In human patients, blood coagulation disorders often associate with cancer, even in its early stages. Recently, in vitro and in vivo experimental models have shown that oncogene expression, or inactivation of tumour suppressor genes, upregulate genes that control blood coagulation. These studies suggest that activation of blood clotting, leading to peritumoral fibrin deposition, is instrumental in cancer development. Fibrin can indeed build up a provisional matrix, supporting the invasive growth of neoplastic tissues and blood vessels. Interference with blood coagulation can thus be considered as part of a multifaceted therapeutic approach to cancer. Received 30 November 2005; received after revision 7 February 2005; accepted 8 February 2006     

点突变ras癌基因全cDNA真核表达重组体的构建及诱导NIH3T3细胞转化的研究

将活化癌基因Ｔ２４－ｒａｓ的全ｃＤＮＡ序列正向插入真核载体ｐＭＡＭｎｅｏ，构建成重组质粒ｐＭＡＭｎｅｏ－Ｔ２４－ｒａｓ．将该质粒转染ＮＩＨ３Ｔ３细胞，通过药物筛选，建成细胞系３Ｔ３（Ｔ２４）．Ｓｏｕｔｈｅｒｎ杂交证明外源Ｔ２４－ｒａｓ基因已整合于受体细胞染色体中．３Ｔ３（Ｔ２４）细胞表现出形态学方面的明显变化：具失去接触抑制能力，且在裸鼠体内致瘤等恶性行为．本文构建的Ｔ２４－ｒａｓ基因真核表达重组体和建立的转化细胞系可用于肿瘤的诊断、预防、治疗及抗肿瘤药物的筛选、评估等研究．     

食管癌相关癌基因研究进展

食管癌的发生发展涉及多个癌基因改变,本文就近年来研究热点的myc基因、C-erbB-2基因、ras 基因、MTA1 、MDM2 、CyclinD基因在食管癌中发生的变化作了简要阐述,为食管癌的早期诊断、预后评估及基因诊治探索可行途径.     

癌症与结构基因组学研究

综述了结构基因组学的研究进展,在基因组学水平上对癌症的研究现状及其在癌症研 究中的应用.     

胆囊癌组织中P21、P53、BCL—2癌基因蛋白的表达及PCNA半定量分析

采用免疫组织化学 Ａ Ｂ Ｃ 法,测定了４０ 例胆囊癌和８ 例胆囊腺瘤性息肉组织中 Ｐ２１ 、 Ｐ５３ 、 Ｂ Ｃ Ｌ－ ２ 癌基因蛋白的表达情况及增殖细胞核抗原的表达强度．结果显示： Ｐ２１ 、 Ｐ５３ 、 Ｂ Ｃ Ｌ－２ 蛋白阳性表达率在胆囊癌组织中分别为５２ ．５ ％ 、５２ ．５ ％ 和７０ ％ ．在胆囊腺瘤性息肉中分别为０ ％ 、０ ％ 和１００ ％ ．提示：检测 Ｐ２１ 、 Ｐ５３ 、 Ｂ Ｃ Ｌ－ ２ 蛋白表达情况对胆囊癌与胆囊腺瘤性息肉的鉴别诊断有一定意义; Ｐ２１ 、 Ｐ５３ 蛋白表达可能是胆囊癌预后不良的重要指标     

DNA甲基化在肿瘤形成中的作用（综述）

DNA甲基化改变是肿瘤细胞中常见的现象,DNA甲基化与肿瘤的发生有密切关系。从以下几方面对此做一综述。（1）简介哺乳动物细胞的DNA甲基化;（2）DNA甲基化与肿瘤基因突变;（3）肿瘤DNA甲基化的基因外作用,其中包括：原癌基因的低甲基化和抑癌基因的高甲基化。     

Notch and cancer: a double-edged sword

The highly conserved Notch signaling pathway plays pleiotropic roles during embryonic development and is important for the regulation of selfrenewing tissues. The physiological functions of this signaling cascade range from stem cell maintenance and influencing cell fate decisions of barely differentiated progenitor cells, to the induction of terminal differentiation processes, all of which have been found to be recapitulated in different forms of cancers. Although Notch signaling has mostly been associated with oncogenic and growth-promoting roles, depending on the tissue type it can also function as a tumor suppressor. Here we describe recent findings on Notch signaling in cancer and tumor angiogenesis, and highlight some of the therapeutic approaches that are currently being developed to interfere with tumor growth and progression. Received 2 April 2007; received after revision 29 June 2007; accepted 2 July 2007     

Effect of P15INK4b/MTS2 on the proliferation of human hepatoma cells SMMC-7721

The full length cDNA coding for P15 INK4b, which is a cyclin-dependent kinase inhibitor, was cloned to plasmid PXJ41-neo (Eco RⅠ/XhoⅠ site) and the new constructed plasmid pXJp15 was obtained. pXJp15 was transferred into the human hepatoma SMMC-7721 cells by lipofectine reagent. After G418 selection, a series of cell lines stably expressing high levels of P15 (named SHT) and the clone containing vector PXJ41-neo only (named SVXJ) were obtained by Northern and Western analysis. The results showed that the proliferation of SHT cells is inhibited compared with that of SVXJ cells. Cell cycle analysis indicated that overexpressing of P15 inhibited the growth of SHT cells by decreasing progrssion of cells from G1 to S and G2 to M phases. The levels of c-Myc and c-Fos were obviously decreased in SHT cells compared with control cells by Western blotting. The decreased expression of oncogene may be one of the molecular mechanisms of the effect of P15 on the proliferation of in SHT cells.     

c－Myb基因反义寡核苷酸对K562细胞增殖和生长的影响

用体外培养技术研究了ｃ－Ｍｙｂ基因的ｍＲＮＡ反义寡核苷酸对慢粒Ｋ５６２红白细胞系增殖和生长的影响。结果显示：ｃ－Ｍｙｂ基因反义寡核苷酸在２０～１６０μｇ／ｍｌ时，对细胞的增殖与集落的形成有明显的抑制作用，提示：ｃ－Ｍｙｂ基因在控制白血病细胞增殖与生长中起重要作用