基于遗传算法的船舶分段运输调度方法

为解决船厂平板运输车搬运船舶分段的日程计划问题,建立了利用最少数量的平板运输车完成分段搬运作业,以及所有分段搬运作业完成时间最小化的两阶段优化模型,提出了基于遗传算法的2种启发式方法以实现模型求解,并用数值实验的方法进行验证.结果表明,所提出的2种方法都能够较好地完成船舶分段运输调度计划,并实现平板运输车的高效利用.     

Expression and self-assembly of Heterocapsa circularisquama RNA virus-like particles synthesized in Pichia pastoris

Heterocapsa circularisquama RNA virus(HcRNAV) is the first single-stranded RNA virus to be characterized that infects dinoflagellates.The ability of HcRNAV coat protein(HcRNAV CP) to self-assemble into virus-like particles(VLPs) in vitro suggested that heterologous expression was possible,and that the VLPs might be ideal nanocontainers for the targeted delivery of genes and chemicals.In this paper,we report the expression of a codon-optimized HcRNAV 109 CP gene in Pichia pastoris and the production of self-assembled HcRNAV VLPs using large-scale fermentation.The HcRNAV 109 CP gene was synthesized according to the codon preference of P.pastoris and cloned into a pPICZA vector.The recombinant plasmid pPICZA-CPsyns was transformed into P.pastoris by electroporation.The resulting yeast colonies were screened by PCR and analyzed for protein expression by SDS polyacrylamide gel electrophoresis.After large-scale fermentation,the yield of HcRNAV CPsyns reached approximately 2.5 g L 1 within 4 d.The HcRNAV VLPs were purified using PEG precipitation followed by cesium chloride density gradient ultracentrifugation,and were subsequently analyzed using UV spectrophotometry and transmission electron microscopy.Fluorescence dye-labeled myoglobin was loaded into the cages of the HcRNAV VLPs and the encapsulation was confirmed by fluorescence spectroscopy.The results point to the possible utilization in pharmacology or nanotechnology of HcRNAV VLPs produced by P.pastoris fermentation.     

设计直动从动件盘形凸轮的新方法

提出了一种利用凸轮与从动件相对运动设计凸轮的新方法（简称相对运动法）,该方法利用凸与从动件的相对速度确定盘形凸轮的压力角和轮廓,相对加速度确定凸轮曲率半径,基于从动件和支承绕凸轮轴线反转的模型,将从动件滚子轴线对凸轮的相对速度和相对加速度转变为绝对速度和绝对加速度,应用矢量法和从动件坐标系中的分量表达法,导出了滚子摆杆盘形凸轮机构的凸轮压力角,轮廓线方程和曲率半径的解析表达式,并通过CAD方法给出了设计实例。     

气—液两相流传递研究

阐述了在气－＝液两相流传递研究方面的工作,包括气升式环流系统中的流体力学和传递、降液膜动下的传热和传质、“载气蒸发”技术的研究和开发等。并由研究惰气气冲击冷剂液浴中放热壁面强化冷却的操作机理,提出了“界面汽化热阱增强传热的原理”。     

漂浮绿藻中缘管浒苔(Ulva linza)鉴定方法的探索(英文)

以研究漂浮绿藻中的缘管浒苔(Ulva linza)为出发点,根据绿藻的形态学特征,选取漂浮绿藻中具有U.linza重要特征的藻体进行形态学、分子生物学的分类学研究.结果显示,具有U.linza重要特征的漂浮藻体大多为LPP复合体(U.linza-procera-prolifera)类群(15个样品),而且采自江苏的DF05、山东的QD03以及浙江的XS01、XS02等4个固着类型的样品也在该分支中.Ulva flexuosa分支中有2个样品(均为采自福建的固着种类),Ulva compressa分枝包含了如东的4个漂浮样品.采自如东内河、射阳沿海池塘的漂浮藻体也均被包含在LPP复合体中.25个样品中只有4个被鉴定为U.linza,与其形态特征研究结果相符合.漂浮绿藻的形态学特征与分子生物学研究结果存在很大差别.这说明单纯地依靠传统分类学特征或分子生物学手段对漂浮绿藻中的缘管浒苔(U.linza)进行分类是不可取的,甚至对漂浮绿藻都不能适用.在同一地点采集到的漂浮绿藻中,大都存在不同形态特征的类型,经过分子生物学分析后被鉴定为不同的种类.根据ITS序列的系统进化树分析结果,绿潮藻中至少存在LPP复合体中的种类以及U.compressa.