产业价值链现代化高质量发展路径研究 ——基于"元宝曲线"的视角

遵循"微笑曲线"和"倒微笑曲线"理论的企业仅聚焦于产业价值链的某一环节获得竞争力,最终要步入差异化内涵的专业化发展路径:遵循"微笑曲线"的企业步入研发、品牌化的路径,而遵循"倒微笑曲线"的企业步入制造专业化的路径.这两种模式皆具有价值创造模式唯一化和高附加值赢利点单一化的弊端.在工业互联网、大数据、云计算等新一代信息技术的影响下,"微笑曲线"逐步发生变化,进一步升级为"元宝曲线"."元宝曲线"集合了"微笑曲线"和"倒微笑曲线"的长处,并在此基础上逐步实现研发专业化、制造专业化、品牌专业化三者的价值融合和价值共生,进而极大地推动企业盈利模式的丰富化和多样化.企业若能在研发、制造、营销三个环节运用创新技术,且相互联系,彼此促进,便可不断提高价值链的整体竞争力,进而逐步增加企业的盈利点、价值分布点,依靠有关不同业务间的密切协同来最终实现"1+1>2"的企业价值最大化目标,进而为企业探索管理运作、经营业务的优化组合提供较佳的理论工具."元宝曲线"的价值创造理念折射出企业未来的经营理念和产业发展方向,其主要体现为推动企业陆续实现价值的多元化经营,不断创造一系列的赢利点,进而在产业价值链的全链条上实现高附加值、高利润率的增长点.苹果公司的价值创造模式就折射出"元宝曲线"的特征,这主要体现为其在专业化和品牌化之间进行有效集成,依靠研发创新的强化、品牌形象的塑造、音乐商店加软件商业业务模式来不断塑造价值链多元化的增长点,从而追求企业价值最大化.华为公司的价值创造模式聚焦于为客户创造价值的硬件产品开发、品牌形象带来的固定客流量以及与安卓系统合作而形成的软件商业生态系统.华为公司若要塑造自主控制、高度协同的"元宝曲线",必须在继续重视研发、品牌的基础上,打造自身掌控自主产权的软件生态系统,进而塑造由自身控制的完整化、系统化、动态化的产业链结构.     

Molecular function of the novel α7β2 nicotinic receptor

The α7 nicotinic receptor is a promising drug target for neurological and inflammatory disorders. Although it is the homomeric member of the family, a novel α7β2 heteromeric receptor has been discovered. To decipher the functional contribution of the β2 subunit, we generated heteromeric receptors with fixed stoichiometry by two different approaches comprising concatenated and unlinked subunits. Receptors containing up to three β2 subunits are functional. As the number of β2 subunits increases in the pentameric arrangement, the durations of channel openings and activation episodes increase progressively probably due to decreased desensitization. The prolonged activation episodes conform the kinetic signature of α7β2 and may have an impact on neuronal excitability. For activation of α7β2 receptors, an α7/α7 binding-site interface is required, thus indicating that the three β2 subunits are located consecutively in the pentameric arrangement. α7-positive allosteric modulators (PAMs) are emerging as novel therapeutic drugs. The presence of β2 in the pentamer affects neither type II PAM potentiation nor activation by an allosteric agonist whereas it impairs type I PAM potentiation. This first single-channel study provides fundamental basis required to decipher the role and function of the novel α7β2 receptor and opens doors to develop selective therapeutic drugs.     

Macropinocytosis,mTORC1 and cellular growth control

The growth and proliferation of metazoan cells are driven by cellular nutrient status and by extracellular growth factors. Growth factor receptors on cell surfaces initiate biochemical signals that increase anabolic metabolism and macropinocytosis, an actin-dependent endocytic process in which relatively large volumes of extracellular solutes and nutrients are internalized and delivered efficiently into lysosomes. Macropinocytosis is prominent in many kinds of cancer cells, and supports the growth of cells transformed by oncogenic K-Ras. Growth factor receptor signaling and the overall metabolic status of the cell are coordinated in the cytoplasm by the mechanistic target-of-rapamycin complex-1 (mTORC1), which positively regulates protein synthesis and negatively regulates molecular salvage pathways such as autophagy. mTORC1 is activated by two distinct Ras-related small GTPases, Rag and Rheb, which associate with lysosomal membranes inside the cell. Rag recruits mTORC1 to the lysosomal surface where Rheb directly binds to and activates mTORC1. Rag is activated by both lysosomal luminal and cytosolic amino acids; Rheb activation requires phosphoinositide 3-kinase, Akt, and the tuberous sclerosis complex-1/2. Signals for activation of Rag and Rheb converge at the lysosomal membrane, and several lines of evidence support the idea that growth factor-dependent endocytosis facilitates amino acid transfer into the lysosome leading to the activation of Rag. This review summarizes evidence that growth factor-stimulated macropinocytosis is essential for amino acid-dependent activation of mTORC1, and that increased solute accumulation by macropinocytosis in transformed cells supports unchecked cell growth.     

A Method to Visualize the Skeleton Industrial Structure with Input-Output Analysis and Its Application in China,Japan and USA

The paper established a double filtering method (DFM) to visualize the skeleton industrial structure (SIS) of one economy and find its evolution rule. Different with the previous researches, this method is from a new view of industrial conjunctions combined by leading sectors to depict the industrial structure. It was proved that the leading sector selected by DFM must be key sector selected by Hirschman-Rasmussen method. Applied DFM to input-output tables of China, Japan and USA and MFA to Japan and USA, the results analysis showed that DFM could overtake the two main shortcomings of minimum flow analysis (MFA), scratch SIS of each economy with its own characteristics, visualize the general evolution rules of the industrial structure with crisscrossed conjunctions among leading sectors.     

The Documentary Real: Thinking Documentary Aesthetics

In this article we consider the growing interest in recent years in the use of documentary strategies in the wold of contemporary art, film and performing arts and explore some of the central epistemological assumptions underpinning the persistent idea that the documentary should be equated with ‘non-fiction’. Following Stella Bruzzi we argue that if documentary theory maintains objectivity as the primary measure of value, it will inevitably and continuously arrive at the conclusion that the documentary genre is fundamentally flawed. Instead, we propose to move beyond the ‘realist epistemology’ of documentary theory and focus on the ‘documentary real’, i.e. the specific performativity of the reality constructed in and by the documentary genre. In the last paragraphs, we introduce the various articles that address the “documentary real” in this special issue.     

Defining G protein-coupled receptor peptide ligand expressomes and signalomes in human and mouse islets

Introduction

Islets synthesise and secrete numerous peptides, some of which are known to be important regulators of islet function and glucose homeostasis. In this study, we quantified mRNAs encoding all peptide ligands of islet G protein-coupled receptors (GPCRs) in isolated human and mouse islets and carried out in vitro islet hormone secretion studies to provide functional confirmation for the species-specific role of peptide YY (PYY) in mouse islets.

Materials and methods

GPCR peptide ligand mRNAs in human and mouse islets were quantified by quantitative real-time PCR relative to the reference genes ACTB, GAPDH, PPIA, TBP and TFRC. The pathways connecting GPCR peptide ligands with their receptors were identified by manual searches in the PubMed, IUPHAR and Ingenuity databases. Distribution of PYY protein in mouse and human islets was determined by immunohistochemistry. Insulin, glucagon and somatostatin secretion from islets was measured by radioimmunoassay.

Results

We have quantified GPCR peptide ligand mRNA expression in human and mouse islets and created specific signalomes mapping the pathways by which islet peptide ligands regulate human and mouse GPCR signalling. We also identified species-specific islet expression of several GPCR ligands. In particular, PYY mRNA levels were ~ 40,000-fold higher in mouse than human islets, suggesting a more important role of locally secreted Pyy in mouse islets. This was confirmed by IHC and functional experiments measuring insulin, glucagon and somatostatin secretion.

Discussion

The detailed human and mouse islet GPCR peptide ligand atlases will allow accurate translation of mouse islet functional studies for the identification of GPCR/peptide signalling pathways relevant for human physiology, which may lead to novel treatment modalities of diabetes and metabolic disease.

     

Uncoupled PID Control of Coupled Multi-Agent Nonlinear Uncertain systems

In this paper, PID (proportional-integral-derivative) controllers will be designed to solve the tracking problem for a class of coupled multi-agent systems, where each agent is described by a second-order high-dimensional nonlinear uncertain dynamical system, which only has access to its own tracking error information and does not need to communicate with others. This paper will show that a 3-dimensional manifold can be constructed based on the information about the Lipschitz constants of the system nonlinear dynamics, such that whenever the three parameters of each PID controller are chosen from the manifold, the whole multi-agent system can be stabilized globally and the tracking error of each agent approaches to zero asymptotically. For a class of coupled first-order multi-agent nonlinear uncertain systems, a PI controller will be designed to stabilize the whole system.