Thoughts about high chlorine peaks in X-ray microanalysis

Summary In X-ray microanalysis of biological tissues, high Cl-peaks are constantly observable. Higher Cl-peaks in the tissue than in the epon environment suggest that most Cl originates from the tissue. Therefore rough regional estimations of the Cl level could be possible.     

X-ray microanalysis of animal tissues by means of the field emission-scanning electron microscope

Summary With FESEM the X-ray microanalysis of unstained ultrathin sections (600 Å) free of osmium is possible. Deposits in smooth muscle cells of arteriosclerotic aortae show high calcium-peaks already after 80 sec of measurement. No contamination spots were seen after 11 min of measurement on the same point.     

Generation and annotation of the DNA sequences of human chromosomes 2 and 4

Human chromosome 2 is unique to the human lineage in being the product of a head-to-head fusion of two intermediate-sized ancestral chromosomes. Chromosome 4 has received attention primarily related to the search for the Huntington's disease gene, but also for genes associated with Wolf-Hirschhorn syndrome, polycystic kidney disease and a form of muscular dystrophy. Here we present approximately 237 million base pairs of sequence for chromosome 2, and 186 million base pairs for chromosome 4, representing more than 99.6% of their euchromatic sequences. Our initial analyses have identified 1,346 protein-coding genes and 1,239 pseudogenes on chromosome 2, and 796 protein-coding genes and 778 pseudogenes on chromosome 4. Extensive analyses confirm the underlying construction of the sequence, and expand our understanding of the structure and evolution of mammalian chromosomes, including gene deserts, segmental duplications and highly variant regions.