双杂交系统筛选与AID相互作用蛋白的初步研究

AID(APP intracellular domain)是由淀粉样前体蛋白APP经γ-分泌酶切割产生的胞内端，它可能参与细胞凋亡．基因转录等活动．并对阿尔茨海默氏症的形成有一定影响．为进一步研究AID的功能．在本实验中．我们用AID为诱饵蛋白．运用酵母双杂交系统筛选与其结合的蛋白，得到其中两个阳性克隆．一个为RPS21．另一个为FLJ20551．接着又在酵母体系用β-半乳糖苷酶报告基因验证了它们的相互作用．并在哺乳动物细胞中用免疫共沉淀的方法再次验证了它们相互作用的特异性．这些结果表明RPS21和FLJ20551可能通过与AID相互作用而影响阿尔茨海默氏症的形成。     

Interaction of Stathmin-Like 2 Protein with the APP Intracellular Domain

Amyloid precursor protein （APP） plays a critical role in the formation of Alzheimer＇s disease （AD）. The intracellular domain APP （AID） was suggested to cause neurotoxicity in the nucleus. To investigate the functions AID, yeast two-hybrid screening was performed to identify the proteins which interact with AID. The human brain cDNA library was screened using pGBKT7-AID as a bait, and several positive clones were identified. One of them encodes a part of the human stathmin-like 2 protein （STMN2）. The interaction between STMN2 and APP implies that STMN2 may have an important function in APP-mediated AD formation.