ABCB6 is dispensable for erythropoiesis and specifies the new blood group system Langereis

The human ATP-binding cassette (ABC) transporter ABCB6 has been described as a mitochondrial porphyrin transporter essential for heme biosynthesis, but it is also suspected to contribute to anticancer drug resistance, as do other ABC transporters located at the plasma membrane. We identified ABCB6 as the genetic basis of the Lan blood group antigen expressed on red blood cells but also at the plasma membrane of hepatocellular carcinoma (HCC) cells, and we established that ABCB6 encodes a new blood group system (Langereis, Lan). Targeted sequencing of ABCB6 in 12 unrelated individuals of the Lan(-) blood type identified 10 different ABCB6 null mutations. This is the first report of deficient alleles of this human ABC transporter gene. Of note, Lan(-) (ABCB6(-/-)) individuals do not suffer any clinical consequences, although their deficiency in ABCB6 may place them at risk when determining drug dosage.     

含Cd2+离子土壤的ESR测定

采用ESR技术对人工合成混合土和天然高岭土,以及混有Cd2+离子的人工合成混合土和天然高岭土进行了室温、低温条件下的测试分析比较.当土壤中有Cd2+离子存在时,Cd2+离子进入硅层结构中,使硅层结构缺陷更趋明显,同时土壤中的铁的ESR特征也因Cd2+离子的存在而出现变化,在低温态时有铁的精细ESR特征出现,因此这些ESR特征反映了土壤中Cd2+离子对硅层结构缺陷和铁的ESR特征的影响.     

固硫灰中游离CaO分布与消解研究

为了提高固硫灰在水泥和混凝土中的利用率,采用化学分析法和SEM-EDS微观分析法分析了固硫灰中游离CaO的分布规律,并对比分析了高温水浸法和常温陈化法对游离CaO的消解实验效果。实验结果显示:在研究的固硫灰中,80%的游离CaO分布在粒径小于75μm的颗粒中;常温陈化法消解游离CaO的效果优于高温水浸法,陈化7 d时游离CaO的含量降低至1.0%以下。     

Automatic generation of very efficient programs by Generalized Partial Computation

Generalized Partial Computation (GPC) is a program transformation method utilizing partial information about input data, properties of auxiliary functions and the logical structure of a source program. GPC uses both an inference engine such as a theorem prover and a classical partial evaluator to optimize programs. Therefore, GPC is more powerful than classical partial evaluators but harder to implement and control. We have implemented an experimental GPC system called WSDFU (Waseda Simplify-Distribute-Fold-Unfold). This paper discusses the power of the program transformation system, its theorem prover and future works.     

C-terminal truncated glucose transporter is locked into an inward-facing form without transport activity

The facilitated glucose transporters comprise a structurally related family of proteins predicted to have 12 membrane-spanning domains, with the amino terminus, a relatively large middle loop and the carboxy-terminus all oriented towards the cytoplasm. An alternating conformation model has been proposed to explain the mechanism of facilitated glucose transport. To understand the structure-function relationships, especially the role of the intracellular C-terminal domain, we have modified the rabbit equivalent of the erythroid-type transporter, GLUT1 (ref. 18), using complementary DNA to code for a deletion mutant that lacks most (37 out of 42 amino acids) of the intracellular C-terminal domain. This deletion mutant is expressed at the cell surface of Chinese hamster ovary (CHO) cells, but is functionally inactive, probably because it has lost its capacity to alternate in conformation and so is locked into an inward-facing form.     

Macrophage skewing by Phd2 haplodeficiency prevents ischaemia by inducing arteriogenesis

PHD2 serves as an oxygen sensor that rescues blood supply by regulating vessel formation and shape in case of oxygen shortage. However, it is unknown whether PHD2 can influence arteriogenesis. Here we studied the role of PHD2 in collateral artery growth by using hindlimb ischaemia as a model, a process that compensates for the lack of blood flow in case of major arterial occlusion. We show that Phd2 (also known as Egln1) haplodeficient (Phd2(+/-)) mice displayed preformed collateral arteries that preserved limb perfusion and prevented tissue necrosis in ischaemia. Improved arteriogenesis in Phd2(+/-) mice was due to an expansion of tissue-resident, M2-like macrophages and their increased release of arteriogenic factors, leading to enhanced smooth muscle cell (SMC) recruitment and growth. Both chronic and acute deletion of one Phd2 allele in macrophages was sufficient to skew their polarization towards a pro-arteriogenic phenotype. Mechanistically, collateral vessel preconditioning relied on the activation of canonical NF-κB pathway in Phd2(+/-) macrophages. These results unravel how PHD2 regulates arteriogenesis and artery homeostasis by controlling a specific differentiation state in macrophages and suggest new treatment options for ischaemic disorders.     

Long-term eruptive activity at a submarine arc volcano

Three-quarters of the Earth's volcanic activity is submarine, located mostly along the mid-ocean ridges, with the remainder along intraoceanic arcs and hotspots at depths varying from greater than 4,000 m to near the sea surface. Most observations and sampling of submarine eruptions have been indirect, made from surface vessels or made after the fact. We describe here direct observations and sampling of an eruption at a submarine arc volcano named NW Rota-1, located 60 km northwest of the island of Rota (Commonwealth of the Northern Mariana Islands). We observed a pulsating plume permeated with droplets of molten sulphur disgorging volcanic ash and lapilli from a 15-m diameter pit in March 2004 and again in October 2005 near the summit of the volcano at a water depth of 555 m (depth in 2004). A turbid layer found on the flanks of the volcano (in 2004) at depths from 700 m to more than 1,400 m was probably formed by mass-wasting events related to the eruption. Long-term eruptive activity has produced an unusual chemical environment and a very unstable benthic habitat exploited by only a few mobile decapod species. Such conditions are perhaps distinctive of active arc and hotspot volcanoes.     

FGFR-related gene nou-darake restricts brain tissues to the head region of planarians

The study of planarian regeneration may help us to understand how we can rebuild organs and tissues after injury, disease or ageing. The robust regenerative abilities of planarians are based upon a population of totipotent stem cells (neoblasts), and among the organs regenerated by these animals is a well-organized central nervous system. In recent years, methodologies such as whole-mount in situ hybridizations and double-stranded RNA have been extended to planarians with the aim of unravelling the molecular basis of their regenerative capacities. Here we report the identification and characterization of nou-darake (ndk), a gene encoding a fibroblast growth factor receptor (FGFR)-like molecule specifically expressed in the head region of the planarian Dugesia japonica. Loss of function of ndk by RNA interference results in the induction of ectopic brain tissues throughout the body. This ectopic brain formation was suppressed by inhibition of two planarian FGFR homologues (FGFR1 and FGFR2). Additionally, ndk inhibits FGF signalling in Xenopus embryos. The data suggest that ndk may modulate FGF signalling in stem cells to restrict brain tissues to the head region of planarians.     

Abstraction from constructors and functional constructors and their applications

Structures using constructors are of ordinary use in functional programming to represent data structures of unbound size. Lack of associativity of constructors, however, hinders program analyses or efficient executions. This paper describes ideas of abstraction toward constructors, and similarly abstraction from constructing functions, which we call functional constructors. We demonstrate our ideas making program analyses easier and enable transformation to efficient execution.     

Term Structure Forecasting of Government Bond Yields with Latent and Macroeconomic Factors: Do Macroeconomic Factors Imply Better Out‐of‐Sample Forecasts?

This study empirically examines the role of macroeconomic and stock market variables in the dynamic Nelson–Siegel framework with the purpose of fitting and forecasting the term structure of interest rate on the Japanese government bond market. The Nelson–Siegel type models in state‐space framework considerably outperform the benchmark simple time series forecast models such as an AR(1) and a random walk. The yields‐macro model incorporating macroeconomic factors leads to a better in‐sample fit of the term structure than the yields‐only model. The out‐of‐sample predictability of the former for short‐horizon forecasts is superior to the latter for all maturities examined in this study, and for longer horizons the former is still compatible to the latter. Inclusion of macroeconomic factors can dramatically reduce the autocorrelation of forecast errors, which has been a common phenomenon of statistical analysis in previous term structure models. Copyright © 2013 John Wiley & Sons, Ltd.