Effect of New O-Superfamily Conotoxin on Voltage-Activated Currents of Hippocampal Neurons

The effects of a new O-superfamily conotoxin, SO3, on sodium current (INa), transient A-type potassium currents (IA), and delayed rectified potassium currents (IK), were examined in cultured rat hippocampal neurons using the whole-cell patch clamp technique. Addition of SO3 caused a concentration-dependent, rapidly developing, and reversible inhibition of voltage-activated currents. The IC50 values for the blockage of INa, IA, and IK were calculated as 0.49, 33.9, and 7.6 μmol/L, respectively. The determined Hill coefficients were 1.7, 0.6, and 1.2, respectively. These results indicate that SO3 can selectively inhibit neuronal sodium and potassium currents.     

膨润土极限活化量测定方法研究

本文介绍了用工艺试样热湿拉强度、高水湿压强度和膨润土的液性极限来测定膨润土的极限活化量。通过对若干种膨润土极限活化量的测定发现,对同种膨润土,由三种方法测得的极限活化量都基本相同,高水湿压强和液性极限的方法对没有热湿拉强度仪的厂家更为实用.同时还发现各种膨润土的极限活化量并不相同,所以通常活化型砂用膨润土,活化剂加入量固定为膨润土量的4～5%并不都是适宜的.     

乳化渣油粘结剂

为了扩大减压渣油的使用范围,通过渣油的乳化,得到适用于北方冬季用的乳化渣油粘结剂,其性能不低于合脂粘结剂,而且克服了合脂砂的蠕变现象。文中较为详细地介绍了渣油的乳化工艺及乳化渣油砂的性能。     

Effect of monoclonal antibody to bull sperm sp18 on murine fertilization and embryos development

Western blot analysis revealed that one IgG1 monoclonal antibody (mAb) to sp18 family membrane proteins (Mr. 14, 16 and 18 ku) of bovine sperm reacted faintly with protein bands of 14, 18, 22, 30 and 60 ku (reducing) in samples of mouse sperm. The mAb also reacted to protein of egg lysozyme. Using a laser confocal microscope, indirect immunofluorescence (IIP) showed that the sp18 antigens were present in the posterior head of murine sperm. In murine in vitro fertilization (IVF) and embryo development trails, a total of 426 oocytes from C57BL/6 and F1 hybrid strain (CD1 × C57BL/6 cross) of 12 female mice were used in 3 independent trails. After preincubating capacitated sperm with 182 μg/mL of sp18 mAb in the modified TYH IVF medium for 15-20 min, cumulus-oocyte-complexes were introduced. The fertilization rate in sp18 mAb groups was 77.1 %, which was not significantly (P > 0.05) different from the nonspecific mouse IgG (79.2%) and non-IgG (80.3 %) control groups. Fertilized oocytes had been continuously cultured in modifed CZB medium. 100%, 100% and 97.9% of 1-cell embryos developed to 2-cell stage in sp18 mAb, nonspecific mouse IgG and non-IgG group 30 h after the start of fertilization, respectively. In the nonspecific mouse IgG and non-IgG groups, 64.1 % and 64.3% of embryos developed to the 4-cell stage, respectively, but all developing eggs in sp18 mAb groups arrested development in vitro at 2-cell stage. After zonae of 2-cell blocked embryos were enzy-matically removed with 0.5% pronase, detection of sp18 antigens by IIF indicated that the fluorescence scattered on two embryonic cells. For embryos fertilized in vivo and co-cultured with 182 μg/mL sp18 mAb, the numbers of 1-cell embryos reaching the 2-cell and 4-cell stage were 95. 2% and 70. 5%, which were not significantly (P>0.05) different from the control group (92.9% and 77.9%). These results indicate that the sp18 antigens on posterior head of mouse sperm were incorporated into the egg plasma membrane during fertilization, and played an active role in development of murine preimplanta-tion embryo.     

Cloning of novel conotoxin precursor sequences from Conus pulicarius

Conotoxins are small peptides in the venoms of Conus genus with various physiological activities targeting ion channels and receptors in the neuromuscular system. Applying a modified 3'-RACE (Rapid Amplification of cDNA Ends) strategy, two novel precursor cDNA sequences were cloned from a small vermivorous cone snail, Conus pulicarius. The predicted mature peptides were named conotoxin PuIA, and PuIIA respectively. Conotoxin PuIA consists of 30 residues of amino acids and conotoxin PuIIA 28. Both the two conotoxins have the same cysteine framework and are believed to be members of the O-superfamily conotoxins.     

全站仪三角高程测量的精度分析

文章分析了全站仪三角高程测量的方法、精度,指出了在工程中使用全站仪进行高程测量经过平差计算可以很好地满足施工需要.     

New O-superfamily conotoxins fromConus striatus inhabited near Chinese Hainan Island

Conotoxins are short peptide-toxins with specific targets and large diversity. They are useful in analgesia, neuroprotection, detection of some kinds of deseases, and receptor and ion channel study. In order to explore the conotoxin resourses of Chinese oceans, rapid amplification of 3′ cDNA ends (RACE) method was utilized to systemically analyze the O-superfamily conotoxin content ofConus striatus inhabited near Chinese Hainan Island. Six new O-superfamily conopeptides were identified, one of which is highly homologous to MVIIA, an N-type calcium channel antagonist.     

平行轴变齿厚斜齿轮传动接触分析

以平行轴变齿厚斜齿轮传动为研究对象,根据齿轮啮合原理及小、大齿轮的齿面方程,分别建立其标准安装以及存在中心距安装误差、轴线安装误差和综合安装误差时轮齿接触的数学模型,通过Matlab编程进行求解,得到不同安装情况下轮齿的接触轨迹及传动误差并进行了对比分析。结果表明,该齿轮传动对轴线安装误差较敏感,形成了边缘接触并且引起周期性的传动误差 为该齿轮传动的设计与分析奠定了基础。     

低碳合金钢18CrNiMo7-6齿轮弯曲疲劳试验研究与误差分析

齿轮弯曲疲劳试验是评价齿轮弯曲疲劳性能的重要手段,为保证试验结果的准确性,探究试验过程误差对试验结果的影响。根据GB/T 14230—1993《齿轮弯曲疲劳强度试验方法》中的B试验法以跨五齿对称加载方式制定弯曲疲劳试验方案,进行有限元仿真与电阻应变片测试验证试验方案的正确性,采用升降法开展18CrNiMo7-6喷丸齿轮弯曲疲劳试验,分析齿轮几何精度、装夹误差以及设备加载精度对弯曲疲劳试验结果的影响。结果表明：试验方案计算齿根弯曲应力与有限元仿真以及电阻应变测试的结果最大误差为8.7%,满足工程需求;99%可靠度下18CrNiMo7-6喷丸齿轮弯曲疲劳极限为642 MPa,相比国标中MQ级的500 MPa提高28%,标准设计偏保守。齿轮的几何精度以及试验设备精度对弯曲疲劳试验结果影响较大,考虑误差综合作用对试验结果的影响为2.43%。     

Correction of RNA aberrant splice increases foreign gene expression in transgenic mice

Transgenic mice with mammary gland secreting human granulocyte colony stimulating factor (G-CSF) were produced using mice whey acid protein gene promoter. It was found that there was very low expression level in mammary gland. Human G-CSF cDNA was obtained by RT-PCR from transgenic mice mammary gland. Sequence analysis showed that this G-CSF gene deleted the 4th exon, and compared with human G-CSF genomic DNA, there were donor and acceptor splice sites in the deletion fragment. It was considered that the 3rd and 4th introns also delete in G-CSF fragment. The transgenic construct was corrected by deleting the 3rd and 4th introns to construct the minigene, which was used to produce transgenic mice by microinjection. Northern blot showed that G-CSF expression using the new construct increased 5.4 times as that before in transgenic mice. The results suggested that it was possible that RNA aberrant splice result in low expression in transgenic mice.