SARS冠状病毒N蛋白的表达及二级结构预测分析

通过RT-PCR获得SARS冠状病毒N蛋白基因，分别克隆到原核表达载体pET21a，pET32a和pGEX-4T-1中，将3种重组质粒pET2la-N，pET32a-N和pGEX-4T-1-N分别转化大肠杆菌BL21(DE3)，经IPTG诱导，细菌中分别表达出约46kD的重组N蛋白、约60kD的6xHis-N融合蛋白和约70kD的GST-N融合蛋白，表达量分别达总蛋白的45％、40％和30％．进一步的分析表明：6xHis-N融合蛋白在大肠杆菌中为可溶性表达，该可溶性组分占细菌裂解液的70％左右，且能被6xHis抗体所识别．用蛋白分析软件对N蛋白进行了序列分析和二级结构预测．SARS冠状病毒N蛋白在大肠杆菌中的高效可溶性表达，有助于进一步结晶后进行X射线晶体衍射分析其结构与功能．     

二项式光场和原子相互作用模型中的保真度

运用全量子理论对二项式光场和二能级原子相互作用模型中保真度的演化特性进行了研究，发现二项式光场的初始光子数和失谐量对保真度有明显的影响．结果表明，初始时刻二项式光场的平均光子数越大，保真度就越小；失谐量越大，保真度就越大．     

Polya''''定理的推广

将Polya.G关于整函数的复合函数f（g）为有穷级的定理推广到整函数与亚纯函数的复合函数f（g）为有穷级,并得到f（g）为有穷级的一些充分条件.     

抗震结构最优可靠度若干问题研究

从抗震结构(小震不坏，中震可修，大震不倒)的三级设防的可靠性，抗震结构的最优化设计，钢筋混凝土结构施工期、正常使用期和老化期的可靠度等三部分对抗震结构的最优可靠度理论和应用进行了分析和研究．     

构建适应区域经济发展的职教发展模式--以江西县(市)级职业技术学校发展为例

本文针对近年来江西加快工业化进程，实现江西在中部地区崛起，从而使得各县域经济的持续快速发展对中等职业技术教育这一层次及类型的人才的需求非常旺盛，依据江西县域经济发展的条件、特色，有针对性地提出了江西的县(市)级职业技术学校如何改革与发展的对策。     

零级代数体函数涉及重值的Borel点

证明了单位圆内一类零级代数体函数存在涉及重值的Borel点．     

自旋为1/2的粒子在二能级体系中的几何相位

该文利用3种方法计算了自旋为1／2的粒子在二级体系中的几何相位，并得到了相同的结果，但用从方法与Berry方法计算得到的结果相差一负号，同时分析了该系统中从的单个几何相位因子为什么对应Berry的2个几何相因子．     

悬辊制管Ⅱ级管模质量问题分析和改进措施

介绍了悬辊制管的工序过程及从南方购进的悬辊制管Ⅱ级管模存在的质量问题，通过计算分析了引发这些质量问题的原因，并提出了相应的改进措施。     

Original component of grain size index in core sediment from southwestern slope of the South China Sea and its paleoenvironmental implication

Traditional grain size analysis was basically used to discriminate sediment type based on its particle dimension for sedimentary naming[1,2] and to analyze the dynamic feature of transport medium, including water current and wind strength[3―5]. Recently, some patency advances havebeen made by using different methods to separate different grain sizes in various original composition from sediments for searching a new way of paleoenvironmental study[6―8]. Grain size analysis is even playing an …     

Synthesis of a new meso／microporous composite molecular sieve of MCM-41／mordenite

An MCM-41/mordenite composite with twofold porous structure and stepwise-distributed acidity was prepared for the first time by using zeolite mordenite as the silica-alumina source. The composite molecular sieve has been investigated and compared with a mechanical mixture of mordenite and MCM-41 for their structure, acidity and catalytic activity by means of XRD, N2 adsorption and desorption, HRTEM, DTG, NH3-TPD and catalytic reaction. The characterization results show that the structure and property of the composite molecular sieve are quite different from those of the mechanical mixture, which might be ascribed to the incorporation of secondary building units characteristic of zeolite mordenite into the mesoporous walls of the composite which gives rise to the thicker mesoporous walls, the higher hydrothermal stability and more strong acid sites. Furthermore, the new strategy could be used as a new general method for the preparation of catalysts for the reaction system with multifold large molecules, and the results were well confirmed by the dealkylation of C10^ aromatic hydrocarbon.