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81.
82.
Han Xiao-fang Zheng Lian-shuang Xie Yi-min . Department of Chemical Engineering Wuhan University of Technology Wuhan Hubei China . College of Chemical Environmental Engineering Jianghan University Wuhan Hubei China . The State Key Laboratory of Pulp Paper Engineering South China University of Technology Guangzhou Guangdong China 《武汉大学学报:自然科学英文版》2004,9(1):125-128
Xylanisthemajorcomponentofplantcellwallsandthemostabundantpentosaninnature.Xylancontainsβ 1,4 linkedd xylosebackboneswitharabinose,4 O methyl d glucuronicacid,andaceticacidsubstituents[1 ] .Thexylan degradingen zymesincludeendoxylanase(1,4 β d xylanxylanohydrolase;EC3.2 .1.8)and β xylosidase (1,4 β d xylanxylanohydrolase;EC 3.2 .1.37) .xylanasedegradesthexylanbackboneintoxylo bioseandxylooligosaccharides,whileβ xylosidasereleasesxylosylresiduesfromthenonreducingendsofxylooligosaccha… 相似文献
83.
Vanhove M Zakhem M Devreese B Franceschini N Anne C Bebrone C Amicosante G Rossolini GM Van Beeumen J Frère JM Galleni M 《Cellular and molecular life sciences : CMLS》2003,60(11):2501-2509
The CphA metallo--lactamase produced by Aeromonas hydrophila exhibits two zinc-binding sites. Maximum activity is obtained upon binding of one zinc ion, whereas binding of the second zinc ion results in a drastic decrease in the hydrolytic activity. In this study, we analyzed the role of Asn116 and Cys221, two residues of the active site. These residues were replaced by site-directed mutagenesis and the different mutants were characterized. The C221S and C221A mutants were seriously impaired in their ability to bind the first, catalytic zinc ion and were nearly completely inactive, indicating a major role for Cys221 in the binding of the catalytic metal ion. By contrast, the binding of the second zinc ion was only slightly affected, at least for the C221S mutant. Mutation of Asn116 did not lead to a drastic decrease in the hydrolytic activity, indicating that this residue does not play a key role in the catalytic mechanism. However, the substitution of Asn116 by a Cys or His residue resulted in an approximately fivefold increase in the affinity for the second, inhibitory zinc ion. Together, these data suggested that the first zinc ion is located in the binding site involving the Cys221 and that the second zinc ion binds in the binding site involving Asn116 and, presumably, His118 and His196.Received 3 March 2003; received after revision 4 August 2003; accepted 25 August 2003 相似文献
84.
Averna M De Tullio R Capini P Salamino F Pontremoli S Melloni E 《Cellular and molecular life sciences : CMLS》2003,60(12):2669-2678
The amount of calpastatin directly available in cytosol is under the control of [Ca2+] and [cyclic AMP]. Prolonged calpain activation also promotes degradation of calpastatin. The fluctuation of calpastatin concentration in cell soluble fraction is accompanied by an initial decrease in calpastatin gene expression, followed by a fivefold increase in its expression when the inhibitor protein is degraded. This process can be conceptualized as a mechanism to regulate calpastatin availability in the cell. This conclusion is supported by the fact that calpain, the other component of this proteolytic system, undergoes changes in its levels of expression in a much more limited manner. Furthermore, this process can be observed both in cells exposed to different natural stimuli, or in other cell lines. Modification of calpastatin gene expression might represent a new tool for the in vivo control of the regulatory machinery required for the modulation of Ca2+-dependent proteolysis.Received 18 July 2003; received after revision 3 September 2003; accepted 23 September 2003 相似文献
85.
紫外线诱变康宁木霉提高酶活力的研究 总被引:6,自引:1,他引:5
通过对康宁木霉菌种的诱变、筛选和固体发酵培养,研究了培养基、培养时间、诱变时间及pH值对诱变菌所产酶的活力的影响.结果表明,康宁木霉经诱变5min,在pH为6.0的玉米秸PDA培养基上培养72h,产生的C1酶和Cx酶的酶活力较高,C1酶的酶活力由73u/g提高到176u/g,增加了2.41倍,Cx酶的酶活力由798u/g提高到2069u/g,增加了2.59倍. 相似文献
86.
Li De |jia Li Hai |cheng Zou Guo |lin College of Life Sciences Wuhan University Wuhan Hubei China 《武汉大学学报:自然科学英文版》2003,8(3):875-879
0 IntroductionHemoglobin(Hb)isthemajorhemeproteinofredbloodcells(RBCs)andisresponsibleforthetransportofoxygentothetissues.ThefunctionofHbdependsupontheabilityoffer rousironinthehemegrouptobindandreleaseoxygen .Despiteitsprincipalroleasanoxygen carrier,theHbmoleculepossessesdifferentenzymaticactivities[1 ] andamethodwasdevelopedforthedeterminationofHbbasedonitsenzymaticactivityfortheox idationofo phenylenediamine (OPDA)withH2 O2 asanoxi dant[2 ] .Hbasamimeticenzymeofperoxidase ,cancataly… 相似文献
87.
在不同温度下长期养殖双齿多刺蚁,观察其存活率和活动状况,测定了不同温度对其SOD,POD,CAT活力的影响,并进行了保护酶活性和存活率的相关分析.实验表明,在较低和较高温度下其存活率明显低于10-40℃测定值.在40-45℃时,酶的活性随着温度升高急速下降;在-5-10℃时,酶的活性随着温度降低而下降.保护酶活性和存活率密切相关.10℃以下的低温和40℃以上的高温对其有不同程度的伤害,其适宜温区为10-40℃,最适温度为20-30℃. 相似文献
88.
YANG MeiYing MA PengDa LI WenMing LIU JinYing LI Liang ZHU XiaoJuan WANG XingZhi 《科学通报(英文版)》2007,52(9):1205-1211
Bacterium strain PJ3,isolated from wastewater and identified as Arthrobacter sp. bacterium based on its 16S rDNA gene,could use carbazole as the sole carbon,nitrogen and energy source. The genomic library of strain PJ3 was constructed and a positive clone JM109(pUCW402) was screened out for the expression of dioxygenase by the ability to form yellow ring-fission product. A 2,3-dihydroxybiphenyl dioxygenase(23DHBD) gene of 933 bp was found in the 3360 bp exogenous fragment of pUCW402 by GenSCAN software and BLAST analysis. The phylogenetic analysis showed that 23DHBD from strain PJ3 formed a deep branch separate from a cluster containing most known 23DHBD in GenBank. Southern hybridization confirmed for the first time that the 23DHBD gene was from the genomic DNA of Arthrobacter sp. PJ3. In order to test the gene function,recombinant bacterium BL21(pETW-8) was constructed to express 23DHBD. The expression level in BL21(pETW-8) was highest compared with the recombinant bacteria JM109(pUCW402) and strain PJ3. We observed that 23DHBD was not absolute specific. The enzyme activity was higher with 2,3-dihydroxybiphenyl as a substrate than with catechol. The substrate specificity assay suggested that 23DHBD was essential for cleavage of bi-cyclic aromatic compounds during the course of aromatic compound biodegradation in Arthrobacter sp. strain PJ3. 相似文献
89.
对兔毛纤维采用生物酶处理,使其形态结构发生改变,从而提高了兔毛纤维的表面摩擦系数,增加了纤维之间的抱舍力,提高了纤维的可纺性,改善了兔毛纺织品的脱毛现象. 相似文献
90.
半干旱区土壤酶活性与其理化及微生物的关系 总被引:11,自引:1,他引:11
在半干旱典型区域选择了8个不同植物群落样地对4类土壤酶活性及与土壤理化性质、微生物量的关系进行研究.结果表明,各种酶活性受制于水分、盐碱等因素,含量均较低.大多数酶在同一样地各个剖面中变化趋势一致.相关分析表明,影响各种酶活性的土壤物理、化学、生物性状有一定的差异,其中大多与有机质含量、微生物含量有非常密切的关系.用土壤化学性质对各种酶活性进行逐步回归分析,可以得到理想的方程.主成分分析发现,徽生物量和中性磷酸酶在各主成分中均有较大的相关系数,可认为是该地区有代表性的土壤生物特性因子.另外,对土壤表层酶活性和微生物量进行聚类分析,可以将8个样地分为不同的类别. 相似文献